DCAMKL2 (DCLK2) (NM_001040260) Human Untagged Clone

CAT#: SC323532

DCLK2 (untagged)-Kinase deficient mutant (K422M) of Human doublecortin-like kinase 2 (DCLK2), transcript variant 1



  "NM_001040260" in other vectors (7)

CNY 5,616.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal DCLK2 Antibody
    • 100 ug

CNY 5,808.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms CL2; CLICK-II; CLICK2; CLIK2; DCAMKL2; DCDC3; DCDC3B; DCK2
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC323532 sequence for NM_001040260 edited (data generated by NextGen Sequencing)
ATGGCCAGCACCAGGAGTATCGAGCTGGAGCACTTTGAGGAACGGGACAAAAGGCCGCGG
CCGGGGTCGCGGAGAGGGGCCCCCAGCTCCTCCGGGGGCAGCAGCAGCTCGGGCCCCAAG
GGGAACGGGCTCATCCCCAGTCCGGCGCACAGTGCCCACTGCAGCTTCTACCGCACGCGG
ACCCTGCAGGCCCTCAGCTCGGAGAAGAAGGCCAAGAAGGCGCGCTTCTACCGGAACGGG
GACCGCTACTTCAAGGGCCTGGTGTTTGCCATCTCCAGCGACCGCTTCCGGTCCTTCGAT
GCGCTCCTCATAGAGCTCACCCGCTCCCTGTCGGACAACGTGAACCTGCCCCAGGGTGTC
CGCACTATCTACACCATCGACGGCAGCCGGAAGGTCACCAGCCTGGACGAGCTGCTGGAA
GGTGAGAGTTACGTGTGTGCATCCAATGAACCATTTCGTAAAGTCGATTACACCAAAAAT
ATTAATCCAAACTGGTCTGTGAACATCAAGGGTGGGACATCCCGAGCGCTGGCTGCTGCC
TCCTCTGTGAAAAGTGAAGTAAAAGAAAGTAAAGATTTCATCAAACCCAAGTTAGTGACT
GTGATTCGAAGTGGAGTGAAGCCTAGAAAAGCCGTGCGGATCCTTCTGAATAAAAAGACT
GCTCATTCCTTTGAACAAGTCTTAACAGATATCACCGAAGCCATTAAACTAGACTCAGGA
GTCGTCAAGAGGCTCTGCACCCTGGATGGAAAGCAGGTTACTTGTCTGCAAGACTTTTTT
GGTGATGACGATGTTTTTATTGCATGTGGACCAGAAAAATTTCGTTATGCCCAAGATGAC
TTTGTCCTGGATCATAGTGAATGTCGTGTCCTGAAGTCATCTTATTCTCGATCCTCAGCT
GTTAAGTATTCTGGATCCAAAAGCCCTGGGCCCTCTCGACGCAGCAAATCACCAGCTTCA
GTTAATGGAACTCCCAGCAGCCAACTTTCTACTCCTAAATCTACGAAATCCTCCAGTTCC
TCTCCAACTAGTCCAGGAAGTTTCAGAGGATTAAAGNAGATTTCTGCTCATGGCAGATCT
TCTTCCAATGTAAACGGTGGACCTGAGCTTGACCGTTGCATAAGTCCTGAAGGTGTGAAT
GGAAACAGATGCTCTGAATCATCAACTCTTCTTGAGAAATACAAAATTGGAAAGGTCATT
GGTGATGGCAATTTTGCAGTAGTCAAAGAGTGTATAGACAGGTCCACTGGAAAGGAGTTT
GCCCTAATGATTATAGACAAAGCCAAATGTTGTGGAAAGGAACACCTGATTGAGAATGAA
GTGTCAATACTGCGCCGAGTGAAACATCCCAATATCATTATGCTGGTCGAGGAGATGGAA
ACAGCAACTGAGCTCTTTCTGGTGATGGAATTGGTCAAAGGTGGAGATCTCTTTGATGCA
ATTACTTCGTCGACCAAGTACACTGAGAGAGATGGCAGTGCCATGGTGTACAACTTAGCC
AATGCCCTCAGGTATCTCCATGGCCTCAGCATCGTGCACAGAGACATCAAACCAGAGAAT
CTCTTGGTGTGTGAATATCCTGATGGAACCAAGTCTTTGAAACTGGGAGACTTTGGGCTT
GCGACTGTGGTAGAAGGCCCTTTATACACAGTCTGTGGCACACCCACTTATGTGGCTCCA
GAAATCATTGCTGAAACTGGCTATGGCCTGAAGGTGGACATTTGGGCAGCTGGTGTGATC
ACATACATACTTCTCTGTGGATTCCCACCATTCCGAAGTGAGAACAATCTCCAGGAAGAT
CTCTTCGACCAGATCTTGGCTGGGAAGCTGGAGTTTCCGGCCCCCTACTGGGATAACATC
ACGGACTCTGCCAAGGAATTAATCAGTCAAATGCTTCAGGTAAATGTTGAAGCTCGGTGT
ACCGCGGGACAAATCCTGAGTCACCCCTGGGTGTCAGATGATGCCTCCCAGGAGAATAAC
ATGCAAGCTGAGGTGACAGGTAAACTAAAACAGCACTTTAATAATGCGCTCCCCAAACAG
AACAGCACTACCACCGGGGTCTCCGTCATCATGAACACGGCTCTAGATAAGGAGGGGCAG
ATTTTCTGCAGCAAGCACTGTCAAGACAGCGGCAGGCCTGGGATGGAGCCCATCTCTCCA
GTTCCTCCCTCAGTGGAGGAGATCCCTGTGCCTGGGGAAGCAGTCCCGGCCCCCACCCCT
CCGGAATCTCCCACCCCCCACCCTCCTCCCGCTGCCCCGGGTGGTGAGCGGGCAGGAACC
TGGCGCCGCCACCGAGACTGA

Clone variation with respect to NM_001040260.3
1057 c=>n;1268 a=>t
>OriGene 5' read for mutant NM_001040260 unedited
ACCGCCCGTTGAAGCAATGGGCGGTAGGCGTGTACGGAGGGAGGTCTATATAAGCAGAGCTCGTTTAGTG
AACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGCGCGTTAAGG
GCCCTCGCAGTCAGACGTCCCTGCACCGGCGCTCGCACCCTTAGTCGGCCCGGAACGTCTTTTTGCGGAC
GCCCTCGGAGCAGCCGCGATGGCCAGCACCAGGAGTATCGAGCTGGAGCACTTTGAGGAACGGGACAAAA
AGGCCGCGGGCCGGGGTCGCGGAGAGGGGCCCCCCAGCTCCCTCCGGGGGCAGCAGCAGCTCGGGCCCCC
AGGGGGAAACGGGGCTTCATCCCCCAGTCCCGGCGCACAGTGGCCCAACTGGCACCTTCTTACCCCCCCG
CGGACCCTGGCGGGCCCCTCGCCTCGAGAAAAGAAGGCCCAGAAGGGGCCGCCTTTTACCGGAAACGGGG
AACCCGCTCCTTCAGGGGCTGGGGGGTTTGGCCACCTTCCAGCGACCCGTTTCCGGCCTTTTCAATGCCC
CCCCCTTATAGAACCACACCCCCGCCCCTGGTCGAAAAAGGTTAACCCGTGCCCCAGGGGTTCCCCGCTA
TATTTAAAATTTAAGGGGAGCCGGAAGGGGCCCCCCCCCGGCAAAACCTGGGGAAGGGGAAATTTTCGTG
GGCCCCCCCGGAGACCTTTTTGTAAAGTAGATTCCCCCAAATTTTTACCCCAACTGGTCTGGACACACTA
CAGTGGGGGGATACCCCACGCCCTCTGCTTGCTGCCTCTCTGTGGAAAGTATGAGATATGAAGATATGGT
ATTTTTTTTCTCTCCCCCCCCCTATGGGCGGGCGGATGCACACGGGGAGAGGAGAGCCCCTCCTAAGCCC
GCCGCTGCCTCTCTCTATAGAAAAACAGGGTCGTTCTCTTGTGTCAGCTGTATCAGAAGATGATCGCGCG
CGCAGCTCCATTACACTCTCGGCTCGTCTGAAGACGGTGCTTCGCATGAAGAACGACCAGTGC
>SC323532 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TAYTCACTCTTCTTGAGAATACAAAATTGGAAAGGTCATTGGTGATGGCAATTTTGCAGTAGTCAAAGAG
TGTATAGACAGGTCCACTGGAAAGGAGTTTGCCCTAATGATTATAGACAAAGCCAAATGTTGTGGAAAGG
AACACCTGATTGAGAATGAAGTGTCAATACTGCGCCGAGTGAAACATCCCAATATCATTATGCTGGTCGA
GGAGATGGAAACAGCAACTGAGCTCTTTCTGGTGATGGAATTGGT
Restriction Sites Please inquire     
ACCN NM_001040260
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001040260.1, NP_001035350.1
RefSeq Size 3603 bp
RefSeq ORF 2301 bp
Locus ID 166614
UniProt ID Q8N568
Protein Families Druggable Genome, Protein Kinase
Gene Summary This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. Mouse studies show that the DCX gene, another family member, and this gene share function in the establishment of hippocampal organization and that their absence results in a severe epileptic phenotype and lethality, as described in human patients with lissencephaly. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Sep 2010]
Transcript Variant: This variant (1) is known as exon-5-lacking transcript (PMIDs:15611072 and 18075264). It lacks an in-frame coding exon in the 5' region, as compared to variant 2. The resulting isoform (a) lacks an internal segment, as compared to isoform b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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