United States
Germany
Japan
United Kingdom
China
DCAMKL2 (DCLK2) (NM_001040260) Human Untagged Clone
CAT＃: SC323532
DCLK2 (untagged)-Kinase deficient mutant (K422M) of Human doublecortin-like kinase 2 (DCLK2), transcript variant 1
CNY 5,616.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CL2; CLICK-II; CLICK2; CLIK2; DCAMKL2; DCDC3; DCDC3B; DCK2 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323532 sequence for NM_001040260 edited (data generated by NextGen Sequencing)
ATGGCCAGCACCAGGAGTATCGAGCTGGAGCACTTTGAGGAACGGGACAAAAGGCCGCGG CCGGGGTCGCGGAGAGGGGCCCCCAGCTCCTCCGGGGGCAGCAGCAGCTCGGGCCCCAAG GGGAACGGGCTCATCCCCAGTCCGGCGCACAGTGCCCACTGCAGCTTCTACCGCACGCGG ACCCTGCAGGCCCTCAGCTCGGAGAAGAAGGCCAAGAAGGCGCGCTTCTACCGGAACGGG GACCGCTACTTCAAGGGCCTGGTGTTTGCCATCTCCAGCGACCGCTTCCGGTCCTTCGAT GCGCTCCTCATAGAGCTCACCCGCTCCCTGTCGGACAACGTGAACCTGCCCCAGGGTGTC CGCACTATCTACACCATCGACGGCAGCCGGAAGGTCACCAGCCTGGACGAGCTGCTGGAA GGTGAGAGTTACGTGTGTGCATCCAATGAACCATTTCGTAAAGTCGATTACACCAAAAAT ATTAATCCAAACTGGTCTGTGAACATCAAGGGTGGGACATCCCGAGCGCTGGCTGCTGCC TCCTCTGTGAAAAGTGAAGTAAAAGAAAGTAAAGATTTCATCAAACCCAAGTTAGTGACT GTGATTCGAAGTGGAGTGAAGCCTAGAAAAGCCGTGCGGATCCTTCTGAATAAAAAGACT GCTCATTCCTTTGAACAAGTCTTAACAGATATCACCGAAGCCATTAAACTAGACTCAGGA GTCGTCAAGAGGCTCTGCACCCTGGATGGAAAGCAGGTTACTTGTCTGCAAGACTTTTTT GGTGATGACGATGTTTTTATTGCATGTGGACCAGAAAAATTTCGTTATGCCCAAGATGAC TTTGTCCTGGATCATAGTGAATGTCGTGTCCTGAAGTCATCTTATTCTCGATCCTCAGCT GTTAAGTATTCTGGATCCAAAAGCCCTGGGCCCTCTCGACGCAGCAAATCACCAGCTTCA GTTAATGGAACTCCCAGCAGCCAACTTTCTACTCCTAAATCTACGAAATCCTCCAGTTCC TCTCCAACTAGTCCAGGAAGTTTCAGAGGATTAAAGNAGATTTCTGCTCATGGCAGATCT TCTTCCAATGTAAACGGTGGACCTGAGCTTGACCGTTGCATAAGTCCTGAAGGTGTGAAT GGAAACAGATGCTCTGAATCATCAACTCTTCTTGAGAAATACAAAATTGGAAAGGTCATT GGTGATGGCAATTTTGCAGTAGTCAAAGAGTGTATAGACAGGTCCACTGGAAAGGAGTTT GCCCTAATGATTATAGACAAAGCCAAATGTTGTGGAAAGGAACACCTGATTGAGAATGAA GTGTCAATACTGCGCCGAGTGAAACATCCCAATATCATTATGCTGGTCGAGGAGATGGAA ACAGCAACTGAGCTCTTTCTGGTGATGGAATTGGTCAAAGGTGGAGATCTCTTTGATGCA ATTACTTCGTCGACCAAGTACACTGAGAGAGATGGCAGTGCCATGGTGTACAACTTAGCC AATGCCCTCAGGTATCTCCATGGCCTCAGCATCGTGCACAGAGACATCAAACCAGAGAAT CTCTTGGTGTGTGAATATCCTGATGGAACCAAGTCTTTGAAACTGGGAGACTTTGGGCTT GCGACTGTGGTAGAAGGCCCTTTATACACAGTCTGTGGCACACCCACTTATGTGGCTCCA GAAATCATTGCTGAAACTGGCTATGGCCTGAAGGTGGACATTTGGGCAGCTGGTGTGATC ACATACATACTTCTCTGTGGATTCCCACCATTCCGAAGTGAGAACAATCTCCAGGAAGAT CTCTTCGACCAGATCTTGGCTGGGAAGCTGGAGTTTCCGGCCCCCTACTGGGATAACATC ACGGACTCTGCCAAGGAATTAATCAGTCAAATGCTTCAGGTAAATGTTGAAGCTCGGTGT ACCGCGGGACAAATCCTGAGTCACCCCTGGGTGTCAGATGATGCCTCCCAGGAGAATAAC ATGCAAGCTGAGGTGACAGGTAAACTAAAACAGCACTTTAATAATGCGCTCCCCAAACAG AACAGCACTACCACCGGGGTCTCCGTCATCATGAACACGGCTCTAGATAAGGAGGGGCAG ATTTTCTGCAGCAAGCACTGTCAAGACAGCGGCAGGCCTGGGATGGAGCCCATCTCTCCA GTTCCTCCCTCAGTGGAGGAGATCCCTGTGCCTGGGGAAGCAGTCCCGGCCCCCACCCCT CCGGAATCTCCCACCCCCCACCCTCCTCCCGCTGCCCCGGGTGGTGAGCGGGCAGGAACC TGGCGCCGCCACCGAGACTGA Clone variation with respect to NM_001040260.3 1057 c=>n;1268 a=>t >OriGene 5' read for mutant NM_001040260 unedited
ACCGCCCGTTGAAGCAATGGGCGGTAGGCGTGTACGGAGGGAGGTCTATATAAGCAGAGCTCGTTTAGTG AACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGCGCGTTAAGG GCCCTCGCAGTCAGACGTCCCTGCACCGGCGCTCGCACCCTTAGTCGGCCCGGAACGTCTTTTTGCGGAC GCCCTCGGAGCAGCCGCGATGGCCAGCACCAGGAGTATCGAGCTGGAGCACTTTGAGGAACGGGACAAAA AGGCCGCGGGCCGGGGTCGCGGAGAGGGGCCCCCCAGCTCCCTCCGGGGGCAGCAGCAGCTCGGGCCCCC AGGGGGAAACGGGGCTTCATCCCCCAGTCCCGGCGCACAGTGGCCCAACTGGCACCTTCTTACCCCCCCG CGGACCCTGGCGGGCCCCTCGCCTCGAGAAAAGAAGGCCCAGAAGGGGCCGCCTTTTACCGGAAACGGGG AACCCGCTCCTTCAGGGGCTGGGGGGTTTGGCCACCTTCCAGCGACCCGTTTCCGGCCTTTTCAATGCCC CCCCCTTATAGAACCACACCCCCGCCCCTGGTCGAAAAAGGTTAACCCGTGCCCCAGGGGTTCCCCGCTA TATTTAAAATTTAAGGGGAGCCGGAAGGGGCCCCCCCCCGGCAAAACCTGGGGAAGGGGAAATTTTCGTG GGCCCCCCCGGAGACCTTTTTGTAAAGTAGATTCCCCCAAATTTTTACCCCAACTGGTCTGGACACACTA CAGTGGGGGGATACCCCACGCCCTCTGCTTGCTGCCTCTCTGTGGAAAGTATGAGATATGAAGATATGGT ATTTTTTTTCTCTCCCCCCCCCTATGGGCGGGCGGATGCACACGGGGAGAGGAGAGCCCCTCCTAAGCCC GCCGCTGCCTCTCTCTATAGAAAAACAGGGTCGTTCTCTTGTGTCAGCTGTATCAGAAGATGATCGCGCG CGCAGCTCCATTACACTCTCGGCTCGTCTGAAGACGGTGCTTCGCATGAAGAACGACCAGTGC >SC323532 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TAYTCACTCTTCTTGAGAATACAAAATTGGAAAGGTCATTGGTGATGGCAATTTTGCAGTAGTCAAAGAG TGTATAGACAGGTCCACTGGAAAGGAGTTTGCCCTAATGATTATAGACAAAGCCAAATGTTGTGGAAAGG AACACCTGATTGAGAATGAAGTGTCAATACTGCGCCGAGTGAAACATCCCAATATCATTATGCTGGTCGA GGAGATGGAAACAGCAACTGAGCTCTTTCTGGTGATGGAATTGGT |
| Restriction Sites | Please inquire |
| ACCN | NM_001040260 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001040260.1, NP_001035350.1 |
| RefSeq Size | 3603 bp |
| RefSeq ORF | 2301 bp |
| Locus ID | 166614 |
| UniProt ID | Q8N568 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. Mouse studies show that the DCX gene, another family member, and this gene share function in the establishment of hippocampal organization and that their absence results in a severe epileptic phenotype and lethality, as described in human patients with lissencephaly. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Sep 2010] Transcript Variant: This variant (1) is known as exon-5-lacking transcript (PMIDs:15611072 and 18075264). It lacks an in-frame coding exon in the 5' region, as compared to variant 2. The resulting isoform (a) lacks an internal segment, as compared to isoform b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219931 | DCLK2 (Myc-DDK-tagged)-Human doublecortin-like kinase 2 (DCLK2), transcript variant 1 |
CNY 5,608.00 |
|
| RC219931L1 | Lenti-ORF clone of DCLK2 (Myc-DDK-tagged)-Human doublecortin-like kinase 2 (DCLK2), transcript variant 1 |
CNY 8,008.00 |
|
| RC219931L2 | Lenti-ORF clone of DCLK2 (mGFP-tagged)-Human doublecortin-like kinase 2 (DCLK2), transcript variant 1 |
CNY 9,980.00 |
|
| RC219931L3 | Lenti-ORF clone of DCLK2 (Myc-DDK-tagged)-Human doublecortin-like kinase 2 (DCLK2), transcript variant 1 |
CNY 9,980.00 |
|
| RC219931L4 | Lenti-ORF clone of DCLK2 (mGFP-tagged)-Human doublecortin-like kinase 2 (DCLK2), transcript variant 1 |
CNY 9,980.00 |
|
| RG219931 | DCLK2 (tGFP-tagged) - Human doublecortin-like kinase 2 (DCLK2), transcript variant 1 |
CNY 8,930.00 |
|
| SC310962 | DCLK2 (untagged)-Human doublecortin-like kinase 2 (DCLK2), transcript variant 1 |
CNY 14,820.00 |
其它DCAMKL2产品
