SCF (KITLG) (NM_003994) Human Untagged Clone
CAT#: SC312617
KITLG (untagged)-Human KIT ligand (KITLG), transcript variant a
CNY 3,600.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | DCUA; DFNA69; FPH2; FPHH; Kitl; KL-1; MGF; SCF; SF; SHEP7; SLF |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene sequence for NM_003994 edited
GAACAGCTAAACGGAGTCGCCACACCACTGTTTGTGCTGGATCGCAGCGCTGCCTTTCCT TATGAAGAAGACACAAACTTGGATTCTCACTTGCATTTATCTTCAGCTGCTCCTATTTAA TCCTCTCGTCAAAACTGAAGGGATCTGCAGGAATCGTGTGACTAATAATGTAAAAGACGT CACTAAATTGGTGGCAAATCTTCCAAAAGACTACATGATAACCCTCAAATATGTCCCCGG GATGGATGTTTTGCCAAGTCATTGTTGGATAAGCGAGATGGTAGTACAATTGTCAGACAG CTTGACTGATCTTCTGGACAAGTTTTCAAATATTTCTGAAGGCTTGAGTAATTATTCCAT CATAGACAAACTTGTGAATATAGTGGATGACCTTGTGGAGTGCGTGAAAGAAAACTCATC TAAGGATCTAAAAAAATCATTCAAGAGCCCAGAACCCAGGCTCTTTACTCCTGAAGAATT CTTTAGAATTTTTAATAGATCCATTGATGCCTTCAAGGACTTTGTAGTGGCATCTGAAAC TAGTGATTGTGTGGTTTCTTCAACATTAAGTCCTGAGAAAGGGAAGGCCAAAAATCCCCC TGGAGACTCCAGCCTACACTGGGCAGCCATGGCATTGCCAGCATTGTTTTCTCTTATAAT TGGCTTTGCTTTTGGAGCCTTATACTGGAAGAAGAGACAGCCAAGTCTTACAAGGGCAGT TGAAAATATACAAATTAATGAAGAGGATAATGAGATAAGTATGTTGCAAGAGAAAGAGAG AGAGTTTCAAGAAGTGTAATTGTGGCTTGTATCAACACTGTTACTTTCGTACATTGGCTG GTAACAGTTCATGTTTGCTTCATAAATGAAGCAGCTTTAAACAAATTCATATTCTGTCTG GAGTGACAGACCACATCTTTATCTGTTCTTGCTACCCATGACTTTATATGGATGATTCAG AAATTGGAACAGAATGTTTTACTGTGAAACTGGCACTGAATTAATCATCTATAAAGAAGA ACTTGCATGGAGCAGGACTCTATTTTAAGGACTGCGGGACTTGGGTCTCATTTAGAACTT GCAGCTGATGTTGGAAGAGAAAGCACGTGTCTCAGACTGCATGCACCATTTGCATGGCTC CAGAAATGTCTAAATGCTGAAAAAACACCTAGCTTTATTCTTCAGATACAAACTGCAGCC TGTAGTTATCCTGGTCTCTGCAAGTAGATTTCAGCTTGGATAGTGAGGGTAACAATTTTT CTCAAAGGGATCTAGAAAAAATGTTTAAAACTCAGTAGTGTCAGCCACTGTACAGTGTAG AAAGCAGTGGGAACTGTGATTGGATTTGGCAACATGTCAGCTTTATAGTTGCCGATTAGT GATATGGGTCTGATTTCGATCTCTTCCTGATGTAAACCATGCTCACCCATATCCCACTAT ACAAATGCAAATGGTTGCCTGGTTCCATTTATGCAAGGGAGCCAGTACTGAATTATGCCT TGGCAGAGGGGAGACTCCAAAAGAGTCATCGCAGGAAGAAGTTAAGAACACTGAACATCA GAACAGTCTGCCAAGAAGGACATTGGCATCCTGGGAAAGTCCGCCTTTTCCCTTGACCAC TATAGGGTGTATAAATCGTGTTTGCAAAATGTGTTATGATGTGTTTATATTCTAAAACTA TTACAGAGCTATGTAAAGGGACTTAGGAGAAAATGCTGAATGTAAGATGGTCCCATTTCA ATTTCCACCATGGGAGAGCCTAAAAATAAATTATGACATTTAGTATCTAAGGTTAGAAAA CCACGCCCACATGCTAATATGGGTGTTGAAAACTAGGTTACTTATAATGCAAGGAATCAG GAAACTTTAGTTATTTATAGTATAATCACCATTATCTGTTTAAAGGATCCATTTAGTTAA AATCGGGCACTCTATATTCATTAAGGTTTATGAATTAAAAAGAAAGCTTTATGTAGTTAT GCATGTCAGTTTGCTATTTAAAATGTGTGACAGTGTTTGTCATATTAAGAGTGAATTTGG CAGGAATTCCCAAGATGGACATTGTGCTTTTAAACTAGAACTTGTAAGACATTATGTGAA TATCCCTTGCCAATTTTTTTTATAATAAGAAAACATCTGACTAAAGTCAAAGAATGATTT CTTATGGTTTATTTTGATGAAAGTTCTTTTAACATGTCTTGAATGTACACATAAAGGAAT CCAAAGCTTTCCATTCTAACTTAATCTTTGTGATAACATTATTGCCATGTTCTACAACCG TAAGATGACAGTTTTCAATGTAGTGACACAAAAGGGCATGAAAAACTAACTGCTAGCTTT CCTTTCATTTCAAAAGTCCAAGAATTTCTAGTATATTTGGATTTTAGCTTCTGTTCAAAG CAAATCCAGATGCAACTCCAGTAAGTGGCCTTTGCTCTTTTTTGTACCAAAGAGCCCAGA TGATTCCTACAGTCCCTTTCTTCTCTAACATGCTGTGGTTCCTTAAATATGAGTAATTTC TCTAAGATATAACCCAGGTGCTTTGAGAAGCTGCATTAAGGTGTTCAGGCCCTCAGATAT CACATGGTACACTTGATTAGTAATAAAACCAGAGATCAATTTAAATTGCTGATAGGTCCT GTCTCAGTGTGTGGCATTGACTGTTTTCAGGAAAATAGATACAGATTAATATGAGTTATG CGTGTAGGTTGTGTATAGATTGAGAAGATAGATACTTCTCAATCTAGTAGTTTGATTTAT TTAACCAATGGTTTCAGTTTGCTTGAGCATATGAAAATCCTGCTTAATGTGCTTAAGAGT ATAATAAATGTGTACTTTTGTCCTCAAACCTAGTAGCTGGGTTTTAACACTCATGGACAT GGTCTTAATCAATGGAGTTAAATAAACAAATTCAGCAAGTTATTAAATCTGACATGGTAG GAGAGGGGAGATGTGTCCTGCTTATTAAATGTGTTGGTCCATTGAAAGTTACATGGATTG CCAATTTTTAAAACACTAAAGTTGAATAAAATGCATGAACAATAGAAAAATGCTGAACAT TATTTTGGATGCTAGCTGCTTGGACATTAACTGTGTTATTTCTGCTTTGAGATGAAAATA TATATTTATCTTTGCTTATTTTATCCCAGATGTGTTCTGAATATCCTTCTTCATAAATCA TGGAAAACTCACTGCTGAGATAGTAAACCATGAAATCGCCTTTTCAGTTGGTGCCATGTA TCTGACAGTTCCATCTTGGAAGGTTTCAAAATTACCTTTTAAAATGATCTCAGAAGTCTG TAGATTCTCAATGATACTGAAAGCTTTGCACCTCTTTGGTAGAAACCAGGTCTATTTAGA AAATGGCTTTATGATAAATGTTGCCTCCTGAGTGATAATGAAGTGTTCCTGGATATTGTA TTGTAATTTAATGTGCTTACCACACTGCCACATTTTAATGAGTCAGAGAAAAATTAATTT TTCTTCAATACAATAATAGAACAAGTAGCCTATTCTCTTAAAAAGTATGTGAAAAGAAAA TTATGAAAAAATATGCATACCTAATGAAGTATTGGTTTTAGTAAGAATTAAATACATTTC ATTGAGCTTTAAAGTACTTTGGAGAAACTTTGGGGCACGTTTTCCTACTCTAATTCAACT AAAGTTATAAATAAAGAGAAAAACTCATTCAGAAATCATGGATTTTAAAAATATTTTACT GCAGCCAAGTTTTCATTTCAAAATGTAATTTCAGTTTGGAGCTTTTAGGCATTATGTATA TTTAAAAAATATATTCTTCAAAAATGCATTTTGGCATGGTGGGATGGATGTTGCAAAAGA TATCCGGAGCCTCCAGTCTGTCATTAACTGATATGGTAAATCACCTCTCTTCTTTGGGTC TCAATTTTTTATTTATCTATATGGTAAACTCAGAGATCACTCCTTAGGGGTGAGTCCTAT TGCAATATGACCGACAAAGAAGACAAAATAGCATTGAAACTAACCCATACAAAATATCCA ACTCTGGATTCTGTGAATAAGTATCTTGACCATAAAAAGTCATTGCTGTTCTTGTTTCTA ATGTAAATAGTGTCCATTAGTAAAAGTGAAATTCAGTCTTAAGTAGGGTGAATTGGATCA CCATTTACACAAGAGATGGCTTTTTCCTTTGCTTGAATAAACATTTTGGATCACCTCCAA AGAATGAAAACCAGTAGTACGTTTTAGTCATATTAGTCAGGATGAGAAACTATAAGATGT GTGTAACATTTGGAAATGCACCAAAGTGAGCGTTTAAATCTTCTCATTTTATTGAAAACT AAGAGCAGAAAATGTAAAATGCTCATGAAGGTTTTGAATGCCAAAAGATATTTTAGAATC AATTTATAAAGGGGTAATTCATTAATTACACTTTAAAATTGGAAAGTGGGATAAGAAATC TAAAGTAAACCAGCTTATCTTTGAAACAATATTATTTTGAAATTGGCTTTAAAATAAAAC CATTCAGATTGAAATTCTAATTAGCTCATTTGTGGAGTTTGATCACACAATTCATAATGT TGCTGCTTTCCATTAACTAGTCTTGAAATGCCTTTGTTTGTAAAAATAAAATAATGGTAC TTTCATTTTATAACAAGGTGTTTTTTTCAAGAAATAATCCATGCTAAAATGGATATTTGT GATCCTGAAATGTTTACTAAGCATTGTAAATTTATTTATAACTGCCATCTCCAACTACAT CCTTATGATGTTTTTAACAATAAAATTAAAACAACTGTTAAACTAAAAACCACACCGTTT TCCAGTACTTGATCTCTGAGCTACAATACTCACTAAATATAATTTTCCAATCAAAATATT CTATTCTATATTCTAAGGGTTAATATGTGATTATAGTGTCCACTTGCCACCATTTTTTTA AATCAATGGACTTGAAAAGTATTAATTTAGATGGATGCGCAGATATACCCTCAGTTCAGT CATAGATTGGAGTTTGCATATAATAATGTAAATGTATGTCGACACTATTCTAAATAGTTC TATTATGACTGAAATTTAATTAAATAAAAAAGGTTGTAAAATGTGATGTGTATGTGTATA TACTGTATGTGTACTTTTTAAAATAGGTGTATGTCCCAACCCTTTTTTATACAGGTTTGA ATTTAAAATTACATGATATATACATATACTTTATTGTTCTAAATAAAGAATTTTATGCAC TCTCAAAAAAAAAAAAAAAAAAAAAAAAAAA |
Restriction Sites | Please inquire |
ACCN | NM_003994 |
Insert Size | 5200 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | The ORF of this clone has been fully sequenced and found to be a perfect match to NM_003994.4. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_003994.4, NP_003985.2 |
RefSeq Size | 5351 bp |
RefSeq ORF | 738 bp |
Locus ID | 4254 |
UniProt ID | P21583 |
Domains | SCF |
Protein Families | Druggable Genome, Transmembrane |
Protein Pathways | Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Melanogenesis, Pathways in cancer |
Gene Summary | This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (a) lacks an alternate in-frame exon, compared to variant b. Isoform a lacks the primary proteolytic-cleavage site; as a result, the protein encoded by isoform a is largely a membrane bound product. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC213540 | KITLG (Myc-DDK-tagged)-Human KIT ligand (KITLG), transcript variant a |
CNY 3,600.00 |
|
RC213540L1 | Lenti ORF clone of Human KIT ligand (KITLG), transcript variant a, Myc-DDK-tagged |
CNY 6,000.00 |
|
RC213540L2 | Lenti ORF clone of Human KIT ligand (KITLG), transcript variant a, mGFP tagged |
CNY 5,890.00 |
|
RC213540L3 | Lenti ORF clone of Human KIT ligand (KITLG), transcript variant a, Myc-DDK-tagged |
CNY 6,000.00 |
|
RC213540L4 | Lenti ORF clone of Human KIT ligand (KITLG), transcript variant a, mGFP tagged |
CNY 6,000.00 |
|
RG213540 | KITLG (tGFP-tagged) - Human KIT ligand (KITLG), transcript variant a |
CNY 5,200.00 |