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ZNF274 (NM_016325) Human Untagged Clone
CAT＃: SC314227
ZNF274 (untagged)-Human zinc finger protein 274 (ZNF274), transcript variant ZNF274a
CNY 9,980.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HFB101; ZF2; ZKSCAN19; ZSCAN51 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC314227 representing NM_016325.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCTCCAGGCTTCCGACGGCCTGGTCCTGTGAACCAGTGACCTTTGAAGATGTAACACTGGGTTTT ACCCCGGAAGAGTGGGGACTGCTGGACCTCAAACAGAAGTCCCTGTACAGGGAAGTGATGCTGGAGAAC TACAGGAACCTGGTCTCAGTGGAGTATCCTGAGCTCCAGCTGGACCCTAAATTGGATCCTCTTCCTGCT GAGAGTCCCCTAATGAACATTGAGGTTGTTGAGGTCCTCACACTGAACCAGGAGGTGGCTGGTCCCCGG AATGCCCAGATCCAGGCCCTATATGCTGAAGATGGAAGCCTGAGTGCAGATGCCCCCAGTGAGCAGGTC CAACAGCAGGGCAAGCATCCAGGTGACCCTGAGGCCGCGCGCCAGAGGTTCCGGCAGTTCCGTTATAAG GACATGACAGGTCCCCGGGAGGCCCTGGACCAGCTCCGAGAGCTGTGTCACCAGTGGCTACAGCCTAAG GCACGCTCCAAGGAGCAGATCCTGGAGCTGCTGGTGCTGGAGCAGTTCCTAGGTGCACTGCCTGTGAAG CTCCGGACATGGGTGGAATCGCAGCACCCAGAGAACTGCCAAGAGGTGGTGGCCCTGGTAGAGGGTGTG ACCTGGATGTCTGAGGAGGAAGTACTTCCTGCAGGACAACCTGCCGAGGGCACCACCTGCTGCCTCGAG GTCACTGCCCAGCAGGAGGAGAAGCAGGAGGATGCAGCCATCTGCCCAGTGACAGTGCTCCCTGAGGAG CCAGTGACCTTCCAGGATGTGGCTGTGGACTTCAGCCGGGAGGAGTGGGGGCTGCTGGGCCCGACACAG AGGACCGAGTACCGCGATGTGATGCTGGAGACCTTTGGGCACCTGGTCTCTGTGGGGTGGGAGACTACA CTGGAAAATAAAGAGTTAGCTCCAAATTCTGACATTCCTGAGGAAGAACCAGCCCCCAGCCTGAAAGTA CAAGAATCCTCAAGGGATTGTGCCTTGTCCTCTACATTAGAAGATACCTTGCAGGGTGGGGTCCAGGAA GTCCAAGACACAGTGTTGAAGCAGATGGAGTCTGCTCAGGAAAAAGACCTTCCTCAGAAGAAGCACTTT GACAACCGTGAGTCCCAGGCAAACAGTGGTGCTCTTGACACAAACCAAGTTTCGCTCCAGAAAATTGAC AACCCTGAGTCCCAGGCAAACAGTGGCGCTCTTGACACAAACCAAGTTTTGCTCCACAAAATTCCTCCT AGAAAACGATTGCGCAAACGTGACTCACAAGTTAAAAGTATGAAACATAATTCACGTGTAAAAATTCAT CAGAAGAGCTGTGAAAGGCAAAAGGCCAAGGAAGGCAATGGTTGTAGGAAAACCTTCAGTCGGAGTACT AAACAGATTACGTTTATAAGAATTCACAAGGGGAGCCAAGTTTGCCGATGCAGTGAATGTGGTAAAATA TTCCGGAACCCAAGATACTTTTCTGTGCATAAGAAAATCCATACCGGAGAGAGGCCCTATGTGTGTCAA GACTGTGGGAAAGGATTTGTTCAGAGCTCTTCCCTCACACAGCATCAGAGAGTTCATTCTGGAGAGAGA CCATTTGAATGTCAGGAGTGTGGGAGGACCTTCAATGATCGCTCAGCCATCTCCCAGCACCTGAGGACT CACACTGGCGCTAAGCCCTACAAGTGTCAGGACTGTGGAAAAGCCTTCCGCCAGAGCTCCCACCTCATC AGACATCAGAGGACTCACACCGGGGAGCGCCCATATGCATGCAACAAATGTGGAAAGGCCTTCACCCAG AGCTCACACCTTATTGGGCACCAGAGAACCCACAATAGGACAAAGCGAAAGAAGAAACAGCCTACCTCA TAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_016325 |
| Insert Size | 1866 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016325.3 |
| RefSeq Size | 2794 bp |
| RefSeq ORF | 1866 bp |
| Locus ID | 10782 |
| UniProt ID | Q96GC6 |
| Protein Families | Transcription Factors |
| Protein Pathways | Neurotrophin signaling pathway |
| MW | 70.5 kDa |
| Gene Summary | This gene encodes a zinc finger protein containing five C2H2-type zinc finger domains, one or two Kruppel-associated box A (KRAB A) domains, and a leucine-rich domain. The encoded protein has been suggested to be a transcriptional repressor. It localizes predominantly to the nucleolus. Alternatively spliced transcript variants encoding different isoforms exist. These variants utilize alternative polyadenylation signals. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (ZNF274a) lacks one in-frame coding exon, compared to variant ZNF274c. The resulting isoform (a) lacks an internal segment, compared to isoform c. Like isoform c, isoform a contains two KRAB A domains. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220631 | ZNF274 (Myc-DDK-tagged)-Human zinc finger protein 274 (ZNF274), transcript variant ZNF274a |
CNY 4,624.00 |
|
| RC220631L3 | Lenti-ORF clone of ZNF274 (Myc-DDK-tagged)-Human zinc finger protein 274 (ZNF274), transcript variant ZNF274a |
CNY 6,460.00 |
|
| RC220631L4 | Lenti-ORF clone of ZNF274 (mGFP-tagged)-Human zinc finger protein 274 (ZNF274), transcript variant ZNF274a |
CNY 6,460.00 |
|
| RG220631 | ZNF274 (tGFP-tagged) - Human zinc finger protein 274 (ZNF274), transcript variant ZNF274a |
CNY 5,040.00 |
