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NET1 (NM_001047160) Human Untagged Clone
CAT＃: SC315444
NET1 (untagged)-Human neuroepithelial cell transforming 1 (NET1), transcript variant 1
CNY 9,690.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ARHGEF8; NET1A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC315444 representing NM_001047160.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGCCCGAGCTGGCGGCTCAGAAGCAGCCTCGACCGCGGAGGCGAAGCCGCCGGGCCTCTGGGCTC AGCACGGAGGGAGCGACGGGGCCTTCGGCCGACACCTCCGGGTCGGAGCTGGACGGGAGATGTTCCCTT CGGAGAGGCAGCTCCTTCACATTCTTAACACCTGGCCCCAACTGGGACTTCACTTTGAAAAGAAAACGC AGAGAGAAAGATGATGATGTTGTAAGCCTTAGCAGCCTTGATCTGAAGGAGCCAAGCAATAAAAGAGTT CGACCTCTGGCTCGTGTCACGTCCTTGGCAAATTTAATCTCTCCTGTAAGAAATGGAGCTGTCAGACGT TTTGGTCAAACAATACAGTCATTTACCCTTCGTGGTGACCACAGATCCCCAGCCTCTGCCCAGAAGTTT TCTAGCAGGTCAACAGTCCCAACACCCGCCAAGAGAAGGAGCAGTGCACTGTGGTCAGAGATGCTGGAC ATCACCATGAAGGAGTCTCTCACCACCAGGGAGATCAGACGGCAGGAGGCAATATATGAAATGTCCCGA GGTGAACAGGATTTAATTGAGGATCTCAAACTTGCAAGAAAGGCCTACCATGACCCCATGTTAAAGTTG TCCATCATGTCAGAAGAGGAACTCACACATATATTTGGTGATCTGGACTCTTACATACCTCTGCATGAA GATTTGTTGACAAGAATAGGAGAAGCAACCAAGCCTGATGGAACAGTGGAGCAGATTGGTCACATTCTC GTGAGCTGGTTACCGCGCTTGAATGCCTACAGAGGTTACTGTAGTAACCAGCTGGCAGCCAAAGCTCTT CTTGATCAAAAGAAACAGGATCCAAGAGTCCAAGACTTCCTCCAGCGATGTCTCGAGTCTCCCTTCAGT CGAAAACTAGATCTTTGGAGTTTCCTAGATATCCCTCGAAGTCGCCTAGTCAAATACCCTTTACTGTTA AAAGAAATTCTTAAACACACTCCAAAAGAGCACCCTGATGTTCAGCTTCTGGAGGATGCTATATTGATA ATACAGGGAGTCCTCTCTGATATCAACTTGAAGAAAGGTGAATCCGAGTGCCAGTATTACATCGACAAG CTGGAGTACCTGGATGAAAAGCAGAGGGACCCCAGAATCGAAGCGAGCAAAGTGCTGCTGTGCCATGGG GAGCTGCGGAGCAAGAGTGGACATAAACTTTACATTTTCCTGTTTCAAGACATCTTGGTTCTGACTCGG CCCGTCACACGGAACGAACGGCACTCTTACCAGGTTTACCGGCAGCCAATCCCAGTCCAAGAGCTAGTC CTAGAAGACCTGCAGGATGGAGATGTGAGAATGGGAGGCTCCTTTCGAGGAGCTTTCAGTAACTCAGAG AAAGCTAAAAATATCTTTAGAATTCGCTTCCATGACCCCTCTCCAGCCCAGTCTCACACTCTGCAAGCC AATGACGTGTTCCACAAGCAGCAGTGGTTCAACTGTATTCGAGCGGCCATTGCCCCCTTCCAGTCGGCA GGCAGTCCACCTGAGCTGCAGGGCCTGCCGGAGCTGCACGAAGAGTGTGAGGGGAACCACCCCTCTGCG AGGAAACTCACAGCCCAGAGGAGGGCATCCACAGTTTCCAGTGTTACTCAGGTAGAAGTTGATGAAAAC GCTTACAGATGTGGCTCTGGCATGCAGATGGCAGAGGACAGCAAGAGCTTAAAGACACACCAGACACAG CCCGGCATCCGAAGAGCGAGGGACAAAGCCCTTTCTGGTGGCAAACGGAAAGAGACTTTGGTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001047160 |
| Insert Size | 1791 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001047160.2 |
| RefSeq Size | 3995 bp |
| RefSeq ORF | 1791 bp |
| Locus ID | 10276 |
| UniProt ID | Q7Z628 |
| Protein Families | Druggable Genome |
| MW | 67.7 kDa |
| Gene Summary | This gene is part of the family of Rho guanine nucleotide exchange factors. Members of this family activate Rho proteins by catalyzing the exchange of GDP for GTP. The protein encoded by this gene interacts with RhoA within the cell nucleus and may play a role in repairing DNA damage after ionizing radiation. Pseudogenes of this gene are located on the long arms of chromosomes 1, 7 and 18. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012] Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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The nuclear RhoA-exchange factor Net1 interacts with proteins of the Dlg family, affects their localization and influences their tumor suppressor activity
,Garcia-Mata R, Dubash AD, Sharek L, Carr HS, Frost JA, Burridge K.,
Mol Cell Biol. 2007 Dec;27(24):8683-97.
[NET1]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223742 | NET1 (Myc-DDK-tagged)-Human neuroepithelial cell transforming 1 (NET1), transcript variant 1 |
CNY 4,440.00 |
|
| RC223742L1 | Lenti ORF clone of Human neuroepithelial cell transforming 1 (NET1), transcript variant 1, Myc-DDK-tagged |
CNY 6,840.00 |
|
| RC223742L2 | Lenti ORF clone of Human neuroepithelial cell transforming 1 (NET1), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RC223742L3 | Lenti ORF clone of Human neuroepithelial cell transforming 1 (NET1), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC223742L4 | Lenti ORF clone of Human neuroepithelial cell transforming 1 (NET1), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RG223742 | NET1 (tGFP-tagged) - Human neuroepithelial cell transforming 1 (NET1), transcript variant 1 |
CNY 5,040.00 |
