CCDC88C (NM_001080414) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DAPLE; HKRP2; HYC1; KIAA1509; SCA40
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC315901 representing NM_001080414. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGACGTGACAGTCTCGGAGCTCCTGGAGCTCTTCCTGCAGAGCCCGCTGGTGACCTGGGTGAAAACT TTTGGCCCGTTTGGAAGCGGCAGCCAGGACAACCTGACTATGTACATGGATTTAGTGGACGGCATCTTT TTGAACCAAATTATGCTGCAAATAGATCCCAGGCCCACAAATCAACGCATCAATAAGCACGTCAACAAT GATGTGAACCTTCGCATTCAGAATTTGACCATCTTGGTGAGAAACATTAAGACCTACTACCAGGAAGTT CTCCAGCAGCTGATTGTAATGAATTTGCCCAATGTTTTGATGATTGGCAGAGACCCACTGTCTGGGAAG AGCATGGAGGAAATCAAGAAGGTGCTGCTGCTGGTGCTGGGCTGTGCTGTCCAGTGTGAGAGGAAAGAG GAGTTCATTGAAAGAATCAAACAGCTGGACATTGAGACCCAGGCTGGCATCGTGGCCCATATCCAGGAG GTGACTCACAACCAAGAGAACGTGTTTGACCTGCAGTGGCTGGAGCTGCCCGACGTGGCTCCGGAGGAG CTGGAGGCCCTGTCGAGGAGCATGGTGCTCCACCTGCGGAGGCTCATCGACCAGCGGGACGAGTGCACC GAGCTGATCGTGGACCTCACTCAGGAACGGGACTACCTGCAGGCACAGCATCCACCCAGCCCCATCAAG TCCTCCAGCGCCGACTCCACTCCCAGCCCCACCAGCAGCCTCTCTAGCGAAGACAAGCAGCACCTGGCC GTAGAGCTGGCCGACACCAAGGCCAGGCTGCGGCGCGTCAGGCAGGAGCTGGAGGATAAGACAGAGCAG CTTGTGGACACCAGACATGAGGTGGACCAGCTGGTGCTGGAACTGCAGAAAGTTAAGCAGGAGAACATC CAGCTAGCGGCAGACGCCCGGTCTGCTCGTGCCTATCGAGACGAGCTGGATTCCCTGCGGGAGAAGGCG AACCGCGTGGAGAGGCTGGAGCTGGAGCTGACCCGCTGCAAGGAGAAGCTGCACGACGTGGACTTCTAC AAGGCCCGCATGGAGGAGCTGAGAGAAGATAATATCATTTTAATTGAAACCAAGGCCATGCTGGAGGAA CAGCTGACTGCTGCTCGGGCCCGGGGCGATAAAGTCCATGAGCTGGAAAAGGAGAACCTGCAGCTGAAA TCCAAGCTTCACGACCTGGAATTGGACCGGGACACAGATAAGAAACGAATTGAGGAGCTGCTGGAAGAA AACATGGTCCTTGAGATTGCACAGAAGCAGAGCATGAACGAATCTGCCCACCTTGGCTGGGAGCTGGAG CAGCTGTCCAAGAACGCAGACTTGTCAGACGCCTCCAGGAAGTCGTTTGTGTTTGAGCTGAACGAATGT GCGTCCAGCCGCATCCTGAAGCTGGAGAAGGAGAATCAGAGCCTCCAGAGCACCATCCAGGGGCTGCGG GACGCGTCCCTGGTGTTGGAGGAGAGCGGCCTCAAGTGCGGGGAGCTGGAGAAGGAGAACCACCAGCTC AGCAAGAAGATTGAAAAGTTACAAACCCAGCTGGAGAGAGAAAAGCAGAGCAACCAAGATCTGGAGACC CTCAGTGAGGAGCTGATCAGAGAGAAGGAGCAGCTGCAGAGTGACATGGAGACCCTGAAGGCTGACAAA GCCAGGCAGATCAAGGACCTTGAGCAGGAAAAGGACCACCTCAACCGAGCCATGTGGTCGCTGCGGGAG AGGTCGCAGGTCAGCAGTGAGGCCCGCATGAAAGACGTGGAGAAGGAGAACAAAGCCCTCCACCAGACG GTGACGGAGGCCAATGGCAAGCTCAGCCAGTTGGAGTTTGAGAAGCGGCAGCTGCACAGGGACTTGGAG CAGGCCAAGGAGAAGGGGGAGCGGGCAGAGAAGCTGGAGAGGGAGCTACAGCGACTCCAGGAGGAGAAC GGGAGGCTGGCCAGGAAGGTGACCTCCCTGGAGACAGCCACCGAGAAAGTCGAGGCCCTGGAGCATGAG AGCCAGGGCCTGCAGCTGGAGAACCGGACTCTGAGGAAGTCTCTGGACACCTTGCAGAACGTGTCCCTG CAGCTTGAGGGCCTGGAGCGTGACAACAAGCAGCTGGACGCAGAGAACCTGGAGCTGCGCAGGCTGGTG GAGACCATGCGCTTCACCAGCACCAAGCTGGCACAGATGGAGAGGGAGAACCAGCAGCTGGAGCGTGAG AAGGAGGAGCTGAGGAAGAACGTGGATCTGCTCAAGGCGCTGGGCAAGAAGTCAGAGCGCCTGGAGCTC AGCTACCAGAGCGTGAGCGCTGAGAACCTCCGGCTGCAGCAGAGCCTGGAGAGCAGCAGCCACAAGACG CAGACCTTGGAGAGTGAGCTGGGCGAGCTGGAGGCTGAGCGCCAGGCGCTGCGGCGGGACCTGGAGGCC CTCCGGCTGGCCAATGCACAGTTGGAGGGGGCCGAGAAGGACAGGAAGGCCCTGGAGCAGGAGGTGGCC CAGCTCGAGAAGGATAAGAAGCTGCTGGAGAAGGAGGCCAAGCGGCTGTGGCAGCAGGTGGAGCTCAAG GATGCAGTCTTGGACGATAGCACTGCCAAACTGTCCGCCGTTGAGAAGGAGAGCCGCGCGCTGGACAAG GAGCTGGCCCGCTGCAGGGACGCAGCCGGCAAGCTGAAGGAGCTGGAGAAGGACAACCGGGACCTCACC AAGCAAGTCACCGTGCATGCAAGGACACTGACAACTCTGAGGGAGGACCTGGTGCTCGAGAAGCTGAAG AGCCAGCAGCTCAGCAGTGAGCTGGACAAGCTGAGCCAGGAACTGGAGAAGGTCGGCCTCAACAGGGAG CTGCTGTTGCAGGAGGACGACAGCGGCAGTGACACAAAATACAAGATTTTGGAGGGCAGAAATGAATCA GCATTAAAAACAACACTAGCCATGAAAGAAGAAAAGATTGTGCTCTTAGAAGCACAGATGGAAGAGAAA GCGAGCCTAAATCGCCAGTTAGAGAGTGAGCTGCAGATGCTAAAGAAGGAGTGTGAGACCCTCAGGCAG AACCAGGGAGAGGGGCAGCACTTGCAGAACTCTTTCAAGCACCCTGCGGGGAAGACAGCCGCCAGTCAC CAGGGGAAGGAGGCCTGGGGGCCCGGCCATAAGGAAGCCACCATGGAGCTTCTCCGAGTGAAGGACCGG GCCATCGAGCTGGAGCGGAATAATGCAGCTCTGCAGGCTGAGAAGCAGCTGCTAAAGGAACAGCTGCAG CACCTGGAGACCCAGAACGTGACCTTCAGCAGCCAGATCTTGACACTGCAGAAACAGAGCGCCTTCCTG CAGGAGCACAACACCACACTGCAGACCCAGACCGCCAAGCTGCAGGTGGAGAACTCCACGCTGAGTTCC CAGAGCGCAGCGCTCACCGCGCAGTACACGCTGCTGCAGAACCACCACACGGCCAAGGAGACGGAGAAC GAAAGCCTGCAGAGGCAGCAGGAGCAACTTACAGCGGCCTACGAGGCCCTGCTGCAGGACCACGAGCAC CTGGGCACGCTGCACGAGCGGCAATCGGCCGAGTACGAGGCCCTCATCCGCCAGCACAGCTGCCTAAAG ACACTGCATCGGAATCTGGAGCTGGAGCACAAGGAGCTCGGGGAGAGGCACGGTGACATGCTGAAGCGC AAGGCGGAGCTGGAGGAGCGGGAGAAGGTCTTGACCACTGAGCGAGAGGCGCTGCAGCAGGAGCAGAGG ACAAACGCCCTCGCCATGGGCGAGAACCAGAGGCTGCGGGGCGAGCTGGACAGGGTCAATTTCCTGCAC CACCAGCTGAAGGGGGAGTACGAGGAGCTGCACGCCCACACCAAGGAGCTGAAAACCTCACTGAACAAC GCGCAGCTGGAGCTCAACCGCTGGCAGGCCCGCTTCGACGAGCTGAAGGAGCAGCACCAGACCATGGAC ATCTCGCTGACCAAGCTGGACAACCACTGTGAGCTGCTCTCCCGTCTCAAGGGGAACTTGGAGGAAGAA AATCATCACCTCCTGAGCCAGATCCAGCTGTTGAGCCAGCAGAACCAGATGCTTCTGGAGCAGAACATG GAGAACAAGGAGCAGTACCATGAGGAGCAGAAGCAGTACATAGACAAATTAAATGCCTTACGAAGACAT AAGGAAAAGCTGGAAGAAAAAATCATGGATCAATACAAGTTCTATGATCCTCCTCCAAAGAAGAAGAAC CACTGGATTGGAGCCAAAGCCTTAGTCAAACTCATCAAACCAAAGAAAGAGGGTTCGAGGGAACGCTTA AAATCCACCGTGGACAGCCCTCCCTGGCAGCTGGAGTCCTCAGACCCCGCCTCGCCGGCGGCCTCTCAG CCGCTCAGATCACAGGCCGAGAACCCCGACACCCCCGCACTGGGCTCCAACTGTGCAGAAGAGCGCGAC GCCCACAACGGGTCTGTGGGGAAAGGCCCTGGGGATCTAAAACCAAAGCGAGGCTCCCCACACAGAGGC AGCCTTGACCGCACAGATGCCTCCACCGATCTGGCCATGAGGTCCTGGCCCTCGGAGCTGGGCTCCCGG ACTTGCTCAACTTCAGCCACCACTACAGCCCCTTCCAACTCCACCCCCATCGCCCGGCACCCAGGCCGC ACCAAAGGCTATAACTCAGATGACAACCTCTGTGAGCCATCCCTGGAGTTTGAGGTCCCCAACCACAGG CAGTACGTGTCGCGGCCAAGTAGCTTAGAGAGCAGTAGAAACACATCCAGCAACAGCTCACCTCTTAAC CTAAAAGGCTCCTCCGAGCAGCTCCATGGCCGGTCTGAGAGCTTCAGCAGCGAAGACCTGATCCCCAGC AGGGACCTGGCCACTTTGCCCCGGGAAGCCAGCACACCGGGACGCAACGCCCTCGGCCGCCACGAGTAC CCCTTGCCTCGGAACGGGCCTCTCCCACAGGAGGGTGCCCAGAAGAGGGGCACAGCCCCTCCCTACGTC GGAGTGCGGCCCTGCTCGGCCTCCCCCAGCAGTGAGATGGTCACCTTGGAGGAGTTCCTGGAGGAGAGC AACCGCAGCTCCCCCACCCATGACACTCCCAGTTGCCGGGATGACCTGCTGAGTGACTACTTCCGAAAG GCCAGCGATCCCCCAGCCATCGGAGGCCAACCAGGACCACCTGCCAAGAAAGAAGGGGCCAAGATGCCC ACCAACTTTGTGGCCCCCACCGTCAAAATGGCCGCCCCCACCTCGGAGGGGAGGCCGCTGAAGCCCGGG CAGTACGTAAAGCCAAACTTCAGACTGACTGAGGCCGAGGCCCCACCCAGCGTGGCCCCGAGACAGGCC CAGCCTCCCCAGAGCCTGTCTCTGGGCAGACCCCGGCAGGCTCCGGTGCCCCCAGCTTCCCATGCACCT GCCAGCCGCAGTGCCTCCTTGAGCCGGGCCTTCAGCCTGGCCTCAGCTGACCTTCTCCGGGCCAGCGGG CCAGAGGCCTGCAAACAGGAGTCCCCTCAGAAGCTGGGGGCTCCTGAGGCCTTAGGGGGCAGAGAGACA GGCAGCCACACCCTGCAAAGCCCCGCACCCCCCAGCTCCCATAGCCTGGCCCGGGAGCGGACCCCACTT GTGGGAAAGGCTGGCAGCTCCTGTCAGGGCCCAGGTCCCCGCAGCCGGCCGCTGGACACGAGGCGCTTC TCCCTGGCTCCCCCAAAGGAGGAGAGGCTGGCCCCCCTGCATCAGTCTGCCACAGCCCCCGCCATTGCC ACTGCAGGTGCTGGTGCTGCTGCTGCTGGCAGTGGCAGCAACTCCCAGCTCCTGCACTTCTCACCTGCT GCAGCCCCGGCTGCCAGGACCAAGCCCAAGGCGCCCCCACGCTCAGGGGAGGTGGCCACCATCACCCCT GTCCGGGCAGGGCTCAGCCTCTCAGAGGGAGACGGGGTCCCGGGGCAGGGCTGCAGTGAGGGGCTTCCG GCCAAGAGCCCAGGTCGGTCTCCCGATTTGGCTCCCCACCTCGGCCGGGCCCTGGAGGACTGCAGTCGA GGGAGCGTCTCAAAGAGCAGTCCGGCCTCCCCGGAGCCCGGCGGGGATCCGCAGACCGTGTGGTATGAG TACGGCTGTGTG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII      Plasmid Map
ACCN	NM_001080414
Insert Size	6084 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001080414.3
RefSeq Size	7573 bp
RefSeq ORF	6084 bp
Locus ID	440193
UniProt ID	Q9P219
MW	228.2 kDa
Gene Summary	This gene encodes a ubiquitously expressed coiled-coil domain-containing protein that interacts with the dishevelled protein and is a negative regulator of the Wnt signalling pathway. The protein encoded by this gene has a PDZ-domain binding motif in its C-terminus with which it interacts with the dishevelled protein. Dishevelled is a scaffold protein involved in the regulation of the Wnt signaling pathway. The Wnt signaling pathway plays an important role in embryonic development, tissue maintenance, and cancer progression. Mutations in this gene cause autosomal recessive, primary non-syndromic congenital hydrocephalus; a condition characterized by excessive accumulation of cerebrospinal fluid in the ventricles of the brain. [provided by RefSeq, Jan 2013]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.