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Mannose Phosphate Isomerase (MPI) (NM_002435) Human Untagged Clone
CAT＃: SC316212
MPI (untagged)-Human mannose phosphate isomerase (MPI)
CNY 3,656.00
CNY 6,840.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CDG1B; PMI; PMI1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC316212 sequence for NM_002435 edited (data generated by NextGen Sequencing)
ATGGCCGCTCCGCGAGTATTCCCACTTTCCTGTGCGGTGCAGCAGTATGCCTGGGGGAAG ATGGGTTCCAACAGCGAAGTGGCGCGGCTGTTGGCCAGCAGTGATCCACTGGCCCAGATC GCAGAGGACAAGCCTTATGCAGAGTTGTGGATGGGGACTCACCCCCGAGGGGATGCCAAG ATCCTTGACAACCGCATCTCACAGAAGACCCTAAGCCAGTGGATTGCTGAGAACCAGGAC AGCTTGGGCTCAAAGGTCAAGGACACCTTTAATGGCAACCTGCCCTTCCTCTTCAAAGTG CTCTCAGTTGAAACACCCCTGTCCATCCAGGCACACCCTAACAAGGAGCTGGCAGAGAAG CTGCACCTCCAGGCTCCGCAGCACTACCCCGATGCCAACCACAAGCCAGAGATGGCCATT GCCCTCACCCCCTTCCAGGGCTTGTGTGGCTTCCGGCCAGTTGAGGAGATTGTAACCTTT CTAAAGAAGGTGCCTGAGTTTCAGTTCCTGATTGGAGATGAGGCAGCAACACACCTGAAG CAGACCATGAGCCATGACTCCCAGGCTGTGGCCTCCTCTCTGCAGAGCTGTTTCTCCCAC CTGATGAAGAGTGAGAAGAAGGTGGTGGTGGAACAGCTCAACCTGTTGGTGAAGCGGATC TCCCAGCAAGCGGCTGCCGGAAACAACATGGAGGACATCTTTGGGGAGCTTTTGCTACAG CTGCACCAGCAGTACCCAGGTGATATCGGCTGCTTTGCCATCTACTTCCTGAACCTGCTT ACCCTGAAGCCTGGGGAGGCCATGTTTCTGGAGGCCAACGTACCCCATGCCTACCTGAAA GGAGACTGCGTGGAGTGCATGGCGTGTTCAGACAACACAGTTCGTGCTGGCCTGACACCC AAGTTCATTGATGTGCCAACCCTGTGTGAAATGCTCAGCTATACCCCTAGCTCCAGCAAG GACAGGCTCTTTCTCCCAACACGGAGTCAGGAAGACCCCTACCTCTCAATCTATGACCCC CCTGTACCAGACTTCACCATTATGAAGACGGAGGTCCCTGGCTCTGTCACTGAATACAAG GTCTTGGCACTGGACTCTGCCAGCATCCTCCTGATGGTACAGGGGACAGTAATAGCCAGC ACACCCACAACCCAGACACCAATCCCTCTGCAACGTGGTGGCGTGCTCTTCATTGGGGCC AATGAGAGTGTCTCACTGAAGCTTACTGAGCCGAAGGACCTGCTGATATTCCGTGCCTGC TGTCTGCTGTAA Clone variation with respect to NM_002435.1 >OriGene 5' read for NM_002435 unedited
TACACTTGTATACGACTCCTATAGGGCGGCCCGCGAACTTCGGCACGAGGCGAGCATGGC CGCTCCGCGAGTATTCCCACTTTCCTGTGCGGTGCAGCAGTATGCCTGGGGGAAGATGGG TTCCAACAGCGAAGTGGCGCGGCTGTTGGCCAGCAGTGATCCACTGGCCCAGATCGCAGA GGACAAGCCTTATGCAGAGTTGTGGATGGGGACTCACCCCCGAGGGGATGCCAAGATCCT TGACAACCGCATCTCACAGAAGACCCTAAGCCAGTGGATTGCTGAGAACCAGGACAGCTT GGGCTCAAAGGTCAAGGACACCTTTAATGGCAACCTGCCCTTCCTCTTCAAAGTGCTCTC AGTTGAAACACCCCTGTCCATCCAGGCACACCCTAACAAGGAGCTGGCAGAGAAGCTGCA CCTCCAGGCTCCGCAGCACTACCCCGATGCCAACCACAAGCCAGAGATGGCCATTGCCCT CACCCCCTTCCAGGGCTTGTGTGGCTTCCGGCCAGTTGAGGAGATTGTAACCTTTCTAAA GAAGGTGCCTGAGTTTCAGTTCCTGATTGGAGATGAGGCAGCAACACACCTGAAGCAGAC CATGAGCCATGACTCCCAGGCTGTGGCCTCCTCTCTGCAGAGCTGTTTCTCCCACCTGAT GAAGAGTGAGAAGAAGGTGGTGGTGGAACAGCTCAACCTGTTGGTGAAGCGGATCTCCCA GCAAGCGGCTGCCGGAAACAACATGGAGGACATCTTTGGGGAGCTTTTGCTACAGCTGCA CCAGCAGTACCCAGGTGATATCGGCTGCTTTGCCATCTACTTCCTGAACCTGCTTACCCT GAAGCCTGGGGAGGCCATGTTTCTGGAGGCCAACGTACCCCATGCCTACCTGAAAGGAGA CTGCGTGAGTGCATGGCGTGTTCAGACAACAACAGTTTCGTGCTGGCCCTGACACCCAAG GTCCA >OriGene 3' read for NM_002435 unedited
CGTTGATGCACTTCAGGGCCGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGG ATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTT TTTTTCTCAACACAAGTGATCTTTAATTGCTTTGACAGAACAAGGGTGTAAACAGCGTTA GTCTACAGGCCTACCCTTTCCCTTTCCTCCTCTGCAGCTGAGGAAATGGGCAGTCCCATC GCTAGAACCCTGTTCTTAAAAATTACCTGGTCTCTTGCTCTGACAGATGCTGAGCTAATG CTGGGTCACATATTTAACAGCTAGCCTAAAGCCATAGGCTGGAACAGAGTTGGCAAGAGC CTGGCTCAGGTGTAGCGAGGAGTAGAGAGGGAAGACAAAGGGAGTATCAAACCCCTCACA CGGGCCTCTCCTCCCCAAAGATGGTTCCACCTGGGTGTGTTCAGGAGTCACTACCTTCAC GCTCCCTCCTCCCCCACCCCAGCTCTTCCAGGGTATACCTAAGGGGTCCAGAGCAAGGAA GGGGGCTTGAGCTGGGCCCGAGGAGGTGAGGTTGGCTGGAATTTAGGGTGGCTGGCAGAG GAGAGCTGGGGAGGCTGCAGCCTTTACAGCAGACAGCAGGCACGGAATATCAGCAGGTCC TTCGGCTCAGTAAGCTTCAGTGAGACACTCTCATTGGCCCCAATGAAGAGCACGCCACCA CGTTGCAGAGGGATTGGTGTCTGGGTTGTGGGTGTGCTGGCTATTACTGTCCCCTGTACC ATCAGGAGGATGCTGGCAGAGTCCAGTGCCAAGACCTTGTATTCAGTGACAGAGCCAGGA CCTCCGTCTCATATGGTGAGTCTGGTACAGGGGGGTCATAGATTGAGAGGTAGGGGTCTT CCTGACTCCGTGTTGGGAGAAAAGAAGCCTTGTCCTTGCTGGAGCTAGGGGTATA |
| Restriction Sites | Please inquire |
| ACCN | NM_002435 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002435.1, NP_002426.1 |
| RefSeq Size | 1771 bp |
| RefSeq ORF | 1272 bp |
| Locus ID | 4351 |
| UniProt ID | P34949 |
| Domains | PMI_typeI |
| Protein Families | ES Cell Differentiation/IPS |
| Protein Pathways | Amino sugar and nucleotide sugar metabolism, Fructose and mannose metabolism, Metabolic pathways |
| Gene Summary | Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] Transcript Variant: This variant (1) encodes the longest isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208134 | MPI (Myc-DDK-tagged)-Human mannose phosphate isomerase (MPI) |
CNY 3,656.00 |
|
| RC208134L1 | Lenti ORF clone of Human mannose phosphate isomerase (MPI), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC208134L2 | Lenti ORF clone of Human mannose phosphate isomerase (MPI), mGFP tagged |
CNY 5,890.00 |
|
| RC208134L3 | Lenti ORF clone of Human mannose phosphate isomerase (MPI), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208134L4 | Lenti ORF clone of Human mannose phosphate isomerase (MPI), mGFP tagged |
CNY 5,890.00 |
|
| RG208134 | MPI (tGFP-tagged) - Human mannose phosphate isomerase (MPI) |
CNY 5,256.00 |
|
| SC324017 | MPI (untagged)-Human mannose phosphate isomerase (MPI) |
CNY 3,656.00 |
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