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MAST3 (NM_015016) Human Untagged Clone
CAT＃: SC316595
MAST3 (untagged)-Human microtubule associated serine/threonine kinase 3 (MAST3)
CNY 13,680.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_015016 edited
ATGGACGAGTCGAGCCTCCTGCGGCGCCGCGGGCTCCAGAAGGAGCTGAGCCTGCCACGC CGAGGACGTGGCTGCCGCAGCGGGAACCGCAAGAGCTTGGTGGTAGGAACGCCCTCCCCG ACCCTCTCCCGGCCCCTGTCGCCATTGTCGGTCCCAACGGCAGGCAGCAGCCCCTTGGAT AGTCCTCGGAATTTCTCGGCTGCCTCTGCCCTAAATTTCCCCTTTGCCCGGAGGGCAGAC GGCAGAAGATGGTCCCTCGCGTCTCTCCCATCTTCCGGCTATGGAACCAACACACCCAGC TCCACCCTCTCGTCAAGCTCATCCTCCCGGGAACGTCTCCACCAGCTTCCCTTCCAGCCG ACGCCGGACGAGCTGCACTTCCTGTCCAAGCACTTCCGCAGCTCAGAGAATGTGCTTGAT GAGGAAGGCGGCCGGTCACCCCGCCTCCGACCCCGCTCTCGCAGTCTCAGCCCGGGCCGT GCAACGGGGACCTTCGACAATGAGATTGTCATGATGAATCACGTGTACCGGGAGAGGTTC CCCAAGGCCACAGCACAGATGGAGGGCCGTCTGCAGGAGTTCCTGACGGCCTACGCGCCC GGCGCCCGGCTGGCGCTGGCTGATGGCGTCTTGGGCTTCATCCACCACCAGATCGTCGAG CTGGCCCGAGACTGCTTGGCCAAGTCTGGCGAGAACCTCGTCACCTCCCGCTACTTCCTA GAGATGCAGGAGAAGCTGGAGCGGCTTCTGCAGGATGCCCATGAGCGTTCGGACAGTGAG GAGGTCAGCTTCATCGTCCAGCTTGTCCGGAAACTGCTGATCATCATCTCACGGCCAGCT CGGCTGCTGGAGTGTCTGGAGTTTGACCCTGAGGAATTTTACCACCTGCTGGAGGCGGCT GAGGGCCATGCGCGGGAGGGCCAAGGCATTAAGACTGACCTTCCACAGTACATCATTGGG CAGCTGGGCCTGGCCAAGGACCCCCTGGAGGAGATGGTGCCACTGAGTCACCTCGAAGAA GAACAGCCCCCAGCACCTGAGTCCCCAGAGAGCCGCGCCCTGGTCGGCCAGTCACGGAGG AAGCCATGCGAAAGCGACTTTGAGACCATCAAACTCATTAGCAACGGAGCCTATGGGGCC GTCTACCTGGTGCGGCACCGTGACACACGGCAGCGCTTTGCCATCAAGAAGATCAACAAA CAGAACTTGATCCTGCGTAACCAGATCCAGCAGGTCTTTGTGGAGCGTGACATTCTCACC TTTGCCGAGAACCCCTTTGTGGTCAGCATGTTCTGCTCCTTTGAGACCCGGCGCCACCTA TGTATGGTCATGGAATACGTGGAAGGCGGCGACTGCGCCACGCTCCTGAAGAACATGGGC CCGCTGCCCGTGGACATGGCCCGCCTGTACTTCGCCGAGACGGTGTTGGCGCTGGAGTAC CTGCATAACTATGGCATCGTGCACCGTGACCTCAAACCAGACAATCTGCTCATCACCTCG CTTGGCCACATCAAGCTCACGGACTTCGGCCTGTCCAAGATCGGCCTCATGAGCATGGCC ACCAACCTCTATGAGGGCCACATCGAGAAGGACGCCCGAGAGTTCATCGACAAGCAGGTG TGTGGGACGCCGGAGTACATAGCCCCCGAGGTGATCTTCCGCCAGGGCTATGGGAAGCCA GTGGACTGGTGGGCCATGGGCGTCGTCCTCTATGAGTTTCTGGTGGGCTGCGTGCCTTTC TTTGGAGATACCCCCGAGGAACTCTTCGGTCAGGTGGTCAGCGATGAGATCATGTGGCCA GAGGGAGATGAGGCCCTTCCAGCAGACGCCCAGGACCTCATCACCAGGTTGCTCCGGCAG AGCCCGCTGGACCGTCTGGGCACTGGTGGCACCCACGAAGTGAAGCAGCACCCCTTTTTC CTGGCCCTGGACTGGGCAGGGCTTCTCCGACACAAAGCCGAGTTCGTGCCCCAGCTCGAA GCCGAGGATGATACCAGCTACTTTGACACACGTTCGGAACGTTACCGCCATCTGGGCTCC GAGGACGACGAGACCAATGATGAAGAATCGTCCACAGAGATCCCCCAGTTCTCCTCCTGC TCCCACCGGTTCAGCAAGGTCTACAGCAGCTCTGAGTTCCTGGCCGTCCAGCCCACTCCT ACCTTCGCTGAAAGGAGCTTCAGTGAAGACCGGGAGGAGGGGTGGGAGCGCAGCGAAGTG GACTATGGCCGCCGGCTGAGTGCTGACATCCGGCTGAGGTCCTGGACATCCTCTGGATCC TCCTGTCAGTCATCTTCGTCCCAGCCCGAGCGGGGTCCCAGCCCATCTCTCCTGAATACC ATCAGCCTGGACACAATGCCCAAGTTTGCCTTCTCATCAGAGGATGAGGGGGTAGGCCCA GGCCCTGCAGGCCCCAAGAGGCCCGTCTTCATTCTAGGGGAGCCTGACCCCCCACCAGCG GCCACCCCAGTGATGCCCAAGCCCTCGAGCCTTTCTGCCGACACAGCTGCTCTCAGCCAC GCCCGCCTACGGAGCAATAGCATCGGCGCCCGACACTCCACACCAAGGCCTCTGGATGCC GGCCGGGGCCGCCGCCTTGGGGGCCCAAGAGACCCAGCCCCTGAGAAGTCCAGAGCCTCC TCCAGCGGTGGCAGTGGTGGCGGCAGTGGGGGCCGCGTGCCCAAGTCAGCCTCTGTCTCT GCCCTGTCCCTCATCATCACGGCAGATGATGGCAGCGGCGGCCCCCTCATGAGCCCCCTT TCCCCGCGCTCTCTGTCCTCGAACCCGTCGTCCCGTGACTCTTCGCCGAGCCGAGACCCG TCCCCCGTGTGTGGCAGCCTGCGGCCCCCCATCGTTATCCACAGCTCTGGCAAGAAGTAC GGCTTCAGCCTGCGGGCGATCCGCGTCTACATGGGTGATAGCGACGTCTACACTGTGCAC CACGTCGTCTGGAGTGTGGAGGACGGAAGCCCCGCCCAGGAGGCGGGCCTGCGGGCTGGG GACCTCATCACCCACATCAACGGGGAGTCAGTGCTGGGGCTGGTGCACATGGACGTCGTG GAGCTGCTGCTGAAGAGCGGCAACAAGATATCCCTGCGGACCACAGCCCTGGAGAACACC TCCATCAAGGTGGGCCCCGCCCGGAAGAATGTGGCCAAGGGCCGCATGGCACGCAGGAGC AAGAGGAGCCGTCGGCGGGAGACCCAGGATCGGCGGAAGTCACTTTTCAAGAAGATCTCC AAGCAGACCTCCGTGCTGCACACCAGCCGCAGCTTCTCCTCCGGACTCCACCACTCACTG TCATCCAGTGAGAGCCTCCCCGGCTCGCCCACCCACAGCCTCTCCCCCAGCCCCACCACT CCCTGCCGAAGCCCAGCCCCTGATGTCCCAGCAGATACCACTGCATCCCCACCCAGCGCA TCCCCGAGCTCCAGCAGCCCCGCCTCCCCAGCTGCTGCTGGCCACACCCGCCCCAGCTCC CTGCACGGCCTGGCTGCCAAGCTTGGGCCACCCCGCCCCAAGACTGGCCGCCGCAAGTCC ACCAGCAGCATCCCGCCCTCCCCGCTGGCCTGCCCGCCCATCTCCGCGCCCCCACCCCGC TCGCCCTCGCCCCTGCCCGGGCACCCGCCCGCACCTGCCCGATCCCCGCGGCTGCGCCGG GGCCAGTCAGCTGACAAGCTGGGCACAGGGGAGCGGCTGGATGGGGAGGCGGGGCGGCGC ACTCGTGGGCCAGAGGCCGAGCTCGTGGTCATGCGGCGGCTGCACCTGTCCGAGCGCCGA GACTCCTTCAAGAAGCAGGAGGCCGTGCAGGAGGTTAGCTTCGATGAGCCGCAGGAGGAG GCCACTGGGCTGCCCACCTCAGTGCCACAGATCGCCGTGGAGGGCGAGGAAGCCGTGCCA GTAGCTCTCGGGCCCACCGGAAGAGACTGA |
| Restriction Sites | Please inquire |
| ACCN | NM_015016 |
| Insert Size | 6000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | It is not a varient. This clone may be unstable or toxic at high copy number in common E. coli strain. We recommend using a lower copy number E. coli strain, such as CopyCutter strain (http://www.epibio.com/item.asp?ID=435) for transformation and plasmid preparation. Please be aware that the DNA yield could be low. Additional aliquots of this clone can be ordered from OriGene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015016.1, NP_055831.1 |
| RefSeq Size | 5893 bp |
| RefSeq ORF | 3930 bp |
| Locus ID | 23031 |
| UniProt ID | O60307 |
| Protein Families | Druggable Genome, Protein Kinase |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Genome-wide Expression Profiling Implicates a MAST3-Regulated Gene Set in Colonic Mucosal Inflammation of Ulcerative Colitis Patients
,null,
Inflammatory Bowel Diseases
,PubMed ID 21994190
[MAST3]
|
Documents
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Resources
Other Versions
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| RC219098L2 | Lenti-ORF clone of MAST3 (mGFP-tagged)-Human microtubule associated serine/threonine kinase 3 (MAST3) |
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| RC219098L3 | Lenti-ORF clone of MAST3 (Myc-DDK-tagged)-Human microtubule associated serine/threonine kinase 3 (MAST3) |
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| RC219098L4 | Lenti-ORF clone of MAST3 (mGFP-tagged)-Human microtubule associated serine/threonine kinase 3 (MAST3) |
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| RG219098 | MAST3 (tGFP-tagged) - Human microtubule associated serine/threonine kinase 3 (MAST3) |
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| SC323438 | MAST3 (untagged)-Kinase deficient mutant (K226M) of Human microtubule associated serine/threonine kinase 3 (MAST3) |
CNY 13,680.00 |
