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MAST3 (NM_015016) Human Untagged Clone
CAT＃: SC323438
MAST3 (untagged)-Kinase deficient mutant (K226M) of Human microtubule associated serine/threonine kinase 3 (MAST3)
CNY 13,680.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_015016, the custom clone sequence may differ by one or more nucleotides
ATGGACGAGTCGAGCCTCCTGCGGCGCCGCGGGCTCCAGAAGGAGCTGAGCCTGCCACGCCGAGGACGTG GCTGCCGCAGCGGGAACCGCAAGAGCTTGGTGGTAGGAACGCCCTCCCCGACCCTCTCCCGGCCCCTGTC GCCATTGTCGGTCCCAACGGCAGGCAGCAGCCCCTTGGATAGTCCTCGGAATTTCTCGGCTGCCTCTGCC CTAAATTTCCCCTTTGCCCGGAGGGCAGACGGCAGAAGATGGTCCCTCGCGTCTCTCCCATCTTCCGGCT ATGGAACCAACACACCCAGCTCCACCCTCTCGTCAAGCTCATCCTCCCGGGAACGTCTCCACCAGCTTCC CTTCCAGCCGACGCCGGACGAGCTGCACTTCCTGTCCAAGCACTTCCGCAGCTCAGAGAATGTGCTTGAT GAGGAAGGCGGCCGGTCACCCCGCCTCCGACCCCGCTCTCGCAGTCTCAGCCCGGGCCGTGCAACGGGGA CCTTCGACAATGAGATTGTCATGATGAATCACGTGTACCGGGAGAGGTTCCCCAAGGCCACAGCACAGAT GGAGGGCCGTCTGCAGGAGTTCCTGACGGCCTACGCGCCCGGCGCCCGGCTGGCGCTGGCTGATGGCGTC TTGGGCTTCATCCACCACCAGATCGTCGAGCTGGCCCGAGACTGCTTGGCCAAGTCTGGCGAGAACCTCG TCACCTCCCGCTACTTCCTAGAGATGCAGGAGAAGCTGGAGCGGCTTCTGCAGGATGCCCATGAGCGTTC GGACAGTGAGGAGGTCAGCTTCATCGTCCAGCTTGTCCGGAAACTGCTGATCATCATCTCACGGCCAGCT CGGCTGCTGGAGTGTCTGGAGTTTGACCCTGAGGAATTTTACCACCTGCTGGAGGCGGCTGAGGGCCATG CGCGGGAGGGCCAAGGCATTAAGACTGACCTTCCACAGTACATCATTGGGCAGCTGGGCCTGGCCAAGGA CCCCCTGGAGGAGATGGTGCCACTGAGTCACCTCGAAGAAGAACAGCCCCCAGCACCTGAGTCCCCAGAG AGCCGCGCCCTGGTCGGCCAGTCACGGAGGAAGCCATGCGAAAGCGACTTTGAGACCATCAAACTCATTA GCAACGGAGCCTATGGGGCCGTCTACCTGGTGCGGCACCGTGACACACGGCAGCGCTTTGCCATCAAGAA GATCAACAAACAGAACTTGATCCTGCGTAACCAGATCCAGCAGGTCTTTGTGGAGCGTGACATTCTCACC TTTGCCGAGAACCCCTTTGTGGTCAGCATGTTCTGCTCCTTTGAGACCCGGCGCCACCTATGTATGGTCA TGGAATACGTGGAAGGCGGCGACTGCGCCACGCTCCTGAAGAACATGGGCCCGCTGCCCGTGGACATGGC CCGCCTGTACTTCGCCGAGACGGTGTTGGCGCTGGAGTACCTGCATAACTATGGCATCGTGCACCGTGAC CTCAAACCAGACAATCTGCTCATCACCTCGCTTGGCCACATCAAGCTCACGGACTTCGGCCTGTCCAAGA TCGGCCTCATGAGCATGGCCACCAACCTCTATGAGGGCCACATCGAGAAGGACGCCCGAGAGTTCATCGA CAAGCAGGTGTGTGGGACGCCGGAGTACATAGCCCCCGAGGTGATCTTCCGCCAGGGCTATGGGAAGCCA GTGGACTGGTGGGCCATGGGCGTCGTCCTCTATGAGTTTCTGGTGGGCTGCGTGCCTTTCTTTGGAGATA CCCCCGAGGAACTCTTCGGTCAGGTGGTCAGCGATGAGATCATGTGGCCAGAGGGAGATGAGGCCCTTCC AGCAGACGCCCAGGACCTCATCACCAGGTTGCTCCGGCAGAGCCCGCTGGACCGTCTGGGCACTGGTGGC ACCCACGAAGTGAAGCAGCACCCCTTTTTCCTGGCCCTGGACTGGGCAGGGCTTCTCCGACACAAAGCCG AGTTCGTGCCCCAGCTCGAAGCTGAGGATGATACCAGCTACTTTGACACACGTTCGGAACGTTACCGCCA TCTGGGCTCCGAGGACGACGAGACCAATGATGAAGAATCGTCCACAGAGATCCCCCAGTTCTCCTCCTGC TCCCACCGGTTCAGCAAGGTCTACAGCAGCTCTGAGTTCCTGGCCGTCCAGCCCACTCCTACCTTCGCTG AAAGGAGCTTCAGTGAAGACCGGGAGGAGGGGTGGGAGCGCAGCGAAGTGGACTATGGCCGCCGGCTGAG TGCTGACATCCGGCTGAGGTCCTGGACATCCTCTGGATCCTCCTGTCAGTCATCTTCGTCCCAGCCCGAG CGGGGTCCCAGCCCATCTCTCCTGAATACCATCAGCCTGGACACAATGCCCAAGTTTGCCTTCTCATCAG AGGATGAGGGGGTAGGCCCAGGCCCTGCAGGCCCCAAGAGGCCCGTCTTCATTCTAGGGGAGCCTGACCC CCCACCAGCGGCCACCCCAGTGATGCCCAAGCCCTCGAGCCTTTCTGCCGACACAGCTGCTCTCAGCCAC GCCCGCCTACGGAGCAATAGCATCGGCGCCCGACACTCCACACCAAGGCCTCTGGATGCCGGCCGGGGCC GCCGCCTTGGGGGCCCAAGAGACCCAGCCCCTGAGAAGTCCAGAGCCTCCTCCAGCGGTGGCAGTGGTGG CGGCAGTGGGGGCCGCGTGCCCAAGTCAGCCTCTGTCTCTGCCCTGTCCCTCATCATCACGGCAGATGAT GGCAGCGGCGGCCCCCTCATGAGCCCCCTTTCCCCGCGCTCTCTGTCCTCGAACCCGTCGTCCCGTGACT CTTCGCCGAGCCGAGACCCGTCCCCCGTGTGTGGCAGCCTGCGGCCCCCCATCGTTATCCACAGCTCTGG CAAGAAGTACGGCTTCAGCCTGCGGGCGATCCGCGTCTACATGGGTGATAGCGACGTCTACACTGTGCAC CACGTCGTCTGGAGTGTGGAGGACGGAAGCCCCGCCCAGGAGGCGGGCCTGCGGGCTGGGGACCTCATCA CCCACATCAACGGGGAGTCAGTGCTGGGGCTGGTGCACATGGACGTCGTGGAGCTGCTGCTGAAGAGCGG CAACAAGATATCCCTGCGGACCACAGCCCTGGAGAACACCTCCATCAAGGTGGGCCCCGCCCGGAAGAAT GTGGCCAAGGGCCGCATGGCACGCAGGAGCAAGAGGAGCCGTCGGCGGGAGACCCAGGATCGGCGGAAGT CACTTTTCAAGAAGATCTCCAAGCAGACCTCCGTGCTGCACACCAGCCGCAGCTTCTCCTCCGGACTCCA CCACTCACTGTCATCCAGTGAGAGCCTCCCCGGCTCGCCCACCCACAGCCTCTCCCCCAGCCCCACCACT CCCTGCCGAAGCCCAGCCCCTGATGTCCCAGCAGATACCACTGCATCCCCACCCAGCGCATCCCCGAGCT CCAGCAGCCCCGCCTCCCCAGCTGCTGCTGGCCACACCCGCCCCAGCTCCCTGCACGGCCTGGCTGCCAA GCTTGGGCCACCCCGCCCCAAGACTGGCCGCCGCAAGTCCACCAGCAGCATCCCGCCCTCCCCGCTGGCC TGCCCGCCCATCTCCGCGCCCCCACCCCGCTCGCCCTCGCCCCTGCCCGGGCACCCGCCCGCACCTGCCC GATCCCCGCGGCTGCGCCGGGGCCAGTCAGCTGACAAGCTGGGCACAGGGGAGCGGCTGGATGGGGAGGC GGGGCGGCGCACTCGTGGGCCAGAGGCCGAGCTCGTGGTCATGCGGCGGCTGCACCTGTCCGAGCGCCGA GACTCCTTCAAGAAGCAGGAGGCCGTGCAGGAGGTTAGCTTCGATGAGCCGCAGGAGGAGGCCACTGGGC TGCCCACCTCAGTGCCACAGATCGCCGTGGAGGGCGAGGAAGCCGTGCCAGTAGCTCTCGGGCCCACCGG AAGAGACTGA >OriGene 5' read for mutant NM_015016 unedited
CCCGCCGTCTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCATTTAGGTG ACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGACGAGTCGAGCC TCCTGCGGCGCCGCGGGGGCCAGAAGGAGCTGAGCCTGCCACGCCGAGGACGTGGCTGCCGCAGCGGGAA CCGCAAGAGCTTGGTGGTAGGAACGCCCTCCCCGACCCTCTCCCGGCCCCTGTCGCCATTGTCGGTCCCA ACGGCAGGCAGCAGCCCCCTTGGATAGTCCTCGGAATTTCTCGGGCTGCCTCTGCCCTAAATTTCCCCTT TGCCCGGAGGGCAGACGGCAGAAGATGGGTCCCTCGCGTCTCTCCAATCTTTCCGGCTATGGACCCAACA CACCCCAGCTTCCACCTTCTCGTCAGGCTCATCCTCCCGGGACCGTCTCCACCGCCTTCCCTTCCGGCCA ACCCCGGACAAGCTGCACTTCCTGTCCAAGCACTTCGGCAGTTCAGAAAATGTGCTTAATAAAAAAGGCC GCCCGGTCACCCCCGCCTCGAAACCGCGCTCGCAGATTCACAGCCCGGGGCCGGGGAAAGGGGAGACTCT GAGAATGAGGATAGTCTAGAAGAAAATCCTGTATCCGGGGAAGAGGTTCCCCAGGCCCACACACATAGAG AGGCCCCTCGGAGATTTCGAGGCGTCTACGCCCGGGCCCCGGTGTGCTGTTGCATGTGGCCTCGGGGTCT CCCACACATATCTAGATGTGCCAGAATGTTGTGCCAATTTGGGAGAAACCTGACTCCGCGATTCTCTAAA AGCCGGGAAACTGAGCGGCTTTTAGAGTGCCCGAAGACTCGACGAGGAGATCTAATCTGCACATTCGGAA GTGATATATATCACAGAACATGGGTAGAAGTCTCGAATGAATCGCGAGACTATGCTCTAGACGCACGA >SC323438 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TATCAACTCATTAGCACGGAGCCTATGGGGCCGTCTACCTGGTGCGGCACCGTGACACACGGCAGCGCTT TGCCATCATGAAGATCAACAAACAGAACTTGATCCTGCGTAACCAGATCCAGCAGGTCTTTGTGGAGCGT GACATTCTCACCTTTGCCGAGAACCCCTTTGTGGTCAGCATGTTCTGCTCCTTTGAGACCCGGCGCCACC TATGTATGGTCATGGAATACGTGGAAGGCGGCGACTGCGCCACGC |
| Restriction Sites | Please inquire |
| ACCN | NM_015016 |
| Insert Size | 6000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone may be unstable or toxic at high copy number in common E. coli strain. We recommend using a lower copy number E. coli strain, such as CopyCutter strain (http://www.epibio.com/item.asp?ID=435) for transformation and plasmid preparation. Please be aware that the DNA yield could be low. Additional aliquots of this clone can be ordered from OriGene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015016.1, NP_055831.1 |
| RefSeq Size | 5893 bp |
| RefSeq ORF | 3930 bp |
| Locus ID | 23031 |
| UniProt ID | O60307 |
| Protein Families | Druggable Genome, Protein Kinase |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219098 | MAST3 (Myc-DDK-tagged)-Human microtubule associated serine/threonine kinase 3 (MAST3) |
CNY 13,672.00 |
|
| RC219098L1 | Lenti-ORF clone of MAST3 (Myc-DDK-tagged)-Human microtubule associated serine/threonine kinase 3 (MAST3) |
CNY 16,072.00 |
|
| RC219098L2 | Lenti-ORF clone of MAST3 (mGFP-tagged)-Human microtubule associated serine/threonine kinase 3 (MAST3) |
CNY 14,920.00 |
|
| RC219098L3 | Lenti-ORF clone of MAST3 (Myc-DDK-tagged)-Human microtubule associated serine/threonine kinase 3 (MAST3) |
CNY 14,920.00 |
|
| RC219098L4 | Lenti-ORF clone of MAST3 (mGFP-tagged)-Human microtubule associated serine/threonine kinase 3 (MAST3) |
CNY 14,920.00 |
|
| RG219098 | MAST3 (tGFP-tagged) - Human microtubule associated serine/threonine kinase 3 (MAST3) |
CNY 14,350.00 |
|
| SC316595 | MAST3 (untagged)-Human microtubule associated serine/threonine kinase 3 (MAST3) |
CNY 13,680.00 |
