MPP5 (NM_022474) Human Untagged Clone

CAT#: SC318145

MPP5 (untagged)-Human membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5) (MPP5)



  "NM_022474" in other vectors (7)

CNY 7,220.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
MPP5 mouse monoclonal antibody, clone OTI2D3 (formerly 2D3)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms PALS1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC318145 representing NM_022474.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGACAACATCCCATATGAATGGGCATGTTACAGAGGAATCAGACAGCGAAGTAAAAAATGTTGATCTT
GCATCACCAGAGGAACATCAGAAGCACCGAGAGATGGCTGTTGACTGCCCTGGAGATTTGGGCACCAGG
ATGATGCCAATACGTCGAAGTGCACAGTTGGAGCGTATTCGGCAACAACAGGAGGACATGAGGCGTAGG
AGAGAGGAAGAAGGGAAAAAGCAAGAACTTGACCTTAATTCTTCCATGAGACTTAAGAAACTAGCCCAA
ATTCCTCCAAAGACCGGAATAGATAACCCTATGTTTGATACAGAGGAAGGAATTGTCTTAGAAAGTCCT
CATTATGCTGTGAAAATATTAGAAATAGAAGACTTGTTTTCTTCACTTAAACATATCCAACATACTTTG
GTAGATTCTCAGAGCCAGGAGGATATTTCACTGCTTTTACAACTTGTTCAAAATAAGGATTTCCAGAAT
GCATTTAAGATACACAATGCCATCACAGTACACATGAACAAGGCCAGTCCTCCATTTCCTCTTATCTCC
AACGCACAAGATCTTGCTCAAGAGGTACAAACTGTTTTGAAGCCAGTTCATCATAAGGAAGGACAAGAA
CTAACTGCTTTGCTGAATACTCCACATATTCAGGCACTTTTACTGGCCCACGATAAGGTTGCTGAGCAG
GAAATGCAGCTAGAGCCCATTACAGATGAGAGAGTTTATGAAAGTATTGGCCAGTATGGAGGAGAAACT
GTAAAAATAGTTCGTATAGAAAAGGCTCGTGATATTCCGTTGGGTGCTACAGTTCGTAATGAAATGGAC
TCTGTCATCATTAGCCGGATAGTAAAAGGGGGTGCTGCAGAGAAAAGTGGTCTGTTGCATGAAGGAGAT
GAAGTTCTAGAGATTAATGGCATTGAAATTCGGGGGAAAGATGTCAATGAGGTTTTTGACTTGTTGTCT
GATATGCATGGTACTTTGACTTTTGTCCTGATTCCCAGTCAACAGATCAAGCCGCCTCCTGCCAAGGAA
ACAGTAATCCATGTAAAAGCTCATTTTGACTATGACCCCTCAGATGACCCTTATGTTCCATGTCGAGAG
TTAGGTCTGTCTTTTCAAAAAGGTGATATACTTCATGTGATCAGTCAAGAAGATCCAAACTGGTGGCAG
GCCTACAGGGAAGGGGACGAAGATAATCAACCTCTAGCCGGGCTTGTTCCAGGGAAAAGCTTTCAGCAG
CAAAGGGAAGCCATGAAACAAACCATAGAAGAAGATAAGGAGCCAGAAAAATCAGGAAAACTGTGGTGT
GCAAAGAAGAATAAAAAGAAGAGGAAAAAGGTTTTATATAATGCCAATAAAAATGATGATTATGACAAC
GAGGAGATCTTAACCTATGAGGAAATGTCACTTTATCATCAGCCAGCAAATAGGAAGAGACCTATCATC
TTGATTGGTCCACAGAACTGTGGCCAGAATGAATTGCGTCAGAGGCTCATGAACAAAGAAAAGGACCGC
TTTGCATCTGCAGTTCCTCATACAACCCGGAGTAGGCGAGACCAAGAAGTAGCCGGTAGAGATTACCAC
TTTGTTTCGCGGCAAGCATTCGAGGCAGACATAGCAGCTGGAAAGTTCATTGAGCATGGTGAATTTGAG
AAGAATTTGTATGGAACTAGCATAGATTCTGTACGGCAAGTGATCAACTCTGGCAAAATATGTCTTTTA
AGTCTTCGTACACAGTCATTGAAGACTCTCCGGAATTCAGATTTGAAACCATATATTATCTTCATTGCA
CCCCCTTCACAAGAAAGACTTCGGGCATTATTGGCCAAAGAAGGCAAGAATCCAAAGCCTGAAGAGTTG
AGAGAAATCATTGAGAAGACAAGAGAGATGGAGCAGAACAATGGCCACTACTTTGATACGGCAATTGTG
AATTCCGATCTTGATAAAGCCTATCAGGAATTGCTTAGGTTAATTAACAAACTTGATACTGAACCTCAG
TGGGTACCATCCACTTGGCTGAGGTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_022474
Insert Size 2028 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_022474.3
RefSeq Size 5608 bp
RefSeq ORF 2028 bp
Locus ID 64398
UniProt ID Q8N3R9
Domains SH3, PDZ, L27, Guanylate_kin, GuKc
Protein Families Druggable Genome
Protein Pathways Tight junction
MW 77.3 kDa
Gene Summary This gene encodes a member of the p55-like subfamily of the membrane-associated guanylate kinase (MAGUK) gene superfamily. The encoded protein participates in the polarization of differentiating cells, has been shown to regulate myelinating Schwann cells (PMID: 20237282), and is one of the components of the Crumbs complex in the retina. Mice which express lower levels of the orthologous protein have retinal degeneration and impaired vision (PMID: 22114289). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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