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MPP5 (NM_022474) Human Tagged ORF Clone
CAT＃: RC224752
MPP5 (Myc-DDK-tagged)-Human membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5) (MPP5)
ORF Plasmid: tGFP
"NM_022474" in other vectors (7)
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CNY 4,944.00
CNY 5,800.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PALS1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC224752 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAACATCCCATATGAATGGGCATGTTACAGAGGAATCAGACAGCGAAGTAAAAAATGTTGATCTTG CATCACCAGAGGAACATCAGAAGCACCGAGAGATGGCTGTTGACTGCCCTGGAGATTTGGGCACCAGGAT GATGCCAATACGTCGAAGTGCACAGTTGGAGCGTATTCGGCAACAACAGGAGGACATGAGGCGTAGGAGA GAGGAAGAAGGGAAAAAGCAAGAACTTGACCTTAATTCTTCCATGAGACTTAAGAAACTAGCCCAAATTC CTCCAAAGACCGGAATAGATAACCCTATGTTTGATACAGAGGAAGGAATTGTCTTAGAAAGTCCTCATTA TGCTGTGAAAATATTAGAAATAGAAGACTTGTTTTCTTCACTTAAACATATCCAACATACTTTGGTAGAT TCTCAGAGCCAGGAGGATATTTCACTGCTTTTACAACTTGTTCAAAATAAGGATTTCCAGAATGCATTTA AGATACACAATGCCATCACAGTACACATGAACAAGGCCAGTCCTCCATTTCCTCTTATCTCCAACGCACA AGATCTTGCTCAAGAGGTACAAACTGTTTTGAAGCCAGTTCATCATAAGGAAGGACAAGAACTAACTGCT TTGCTGAATACTCCACATATTCAGGCACTTTTACTGGCCCACGATAAGGTTGCTGAGCAGGAAATGCAGC TAGAGCCCATTACAGATGAGAGAGTTTATGAAAGTATTGGCCAGTATGGAGGAGAAACTGTAAAAATAGT TCGTATAGAAAAGGCTCGTGATATTCCGTTGGGTGCTACAGTTCGTAATGAAATGGACTCTGTCATCATT AGCCGGATAGTAAAAGGGGGTGCTGCAGAGAAAAGTGGTCTGTTGCATGAAGGAGATGAAGTTCTAGAGA TTAATGGCATTGAAATTCGGGGGAAAGATGTCAATGAGGTTTTTGACTTGTTGTCTGATATGCATGGTAC TTTGACTTTTGTCCTGATTCCCAGTCAACAGATCAAGCCGCCTCCTGCCAAGGAAACAGTAATCCATGTA AAAGCTCATTTTGACTATGACCCCTCAGATGACCCTTATGTTCCATGTCGAGAGTTAGGTCTGTCTTTTC AAAAAGGTGATATACTTCATGTGATCAGTCAAGAAGATCCAAACTGGTGGCAGGCCTACAGGGAAGGGGA CGAAGATAATCAACCTCTAGCCGGGCTTGTTCCAGGGAAAAGCTTTCAGCAGCAAAGGGAAGCCATGAAA CAAACCATAGAAGAAGATAAGGAGCCAGAAAAATCAGGAAAACTGTGGTGTGCAAAGAAGAATAAAAAGA AGAGGAAAAAGGTTTTATATAATGCCAATAAAAATGATGATTATGACAACGAGGAGATCTTAACCTATGA GGAAATGTCACTTTATCATCAGCCAGCAAATAGGAAGAGACCTATCATCTTGATTGGTCCACAGAACTGT GGCCAGAATGAATTGCGTCAGAGGCTCATGAACAAAGAAAAGGACCGCTTTGCATCTGCAGTTCCTCATA CAACCCGGAGTAGGCGAGACCAAGAAGTAGCCGGTAGAGATTACCACTTTGTTTCGCGGCAAGCATTCGA GGCAGACATAGCAGCTGGAAAGTTCATTGAGCATGGTGAATTTGAGAAGAATTTGTATGGAACTAGCATA GATTCTGTACGGCAAGTGATCAACTCTGGCAAAATATGTCTTTTAAGTCTTCGTACACAGTCATTGAAGA CTCTCCGGAATTCAGATTTGAAACCATATATTATCTTCATTGCACCCCCTTCACAAGAAAGACTTCGGGC ATTATTGGCCAAAGAAGGCAAGAATCCAAAGCCTGAAGAGTTGAGAGAAATCATTGAGAAGACAAGAGAG ATGGAGCAGAACAATGGCCACTACTTTGATACGGCAATTGTGAATTCCGATCTTGATAAAGCCTATCAGG AATTGCTTAGGTTAATTAACAAACTTGATACTGAACCTCAGTGGGTACCATCCACTTGGCTGAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224752 protein sequence
Red=Cloning site Green=Tags(s) MTTSHMNGHVTEESDSEVKNVDLASPEEHQKHREMAVDCPGDLGTRMMPIRRSAQLERIRQQQEDMRRRR EEEGKKQELDLNSSMRLKKLAQIPPKTGIDNPMFDTEEGIVLESPHYAVKILEIEDLFSSLKHIQHTLVD SQSQEDISLLLQLVQNKDFQNAFKIHNAITVHMNKASPPFPLISNAQDLAQEVQTVLKPVHHKEGQELTA LLNTPHIQALLLAHDKVAEQEMQLEPITDERVYESIGQYGGETVKIVRIEKARDIPLGATVRNEMDSVII SRIVKGGAAEKSGLLHEGDEVLEINGIEIRGKDVNEVFDLLSDMHGTLTFVLIPSQQIKPPPAKETVIHV KAHFDYDPSDDPYVPCRELGLSFQKGDILHVISQEDPNWWQAYREGDEDNQPLAGLVPGKSFQQQREAMK QTIEEDKEPEKSGKLWCAKKNKKKRKKVLYNANKNDDYDNEEILTYEEMSLYHQPANRKRPIILIGPQNC GQNELRQRLMNKEKDRFASAVPHTTRSRRDQEVAGRDYHFVSRQAFEADIAAGKFIEHGEFEKNLYGTSI DSVRQVINSGKICLLSLRTQSLKTLRNSDLKPYIIFIAPPSQERLRALLAKEGKNPKPEELREIIEKTRE MEQNNGHYFDTAIVNSDLDKAYQELLRLINKLDTEPQWVPSTWLR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022474 |
| ORF Size | 2025 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_022474.4 |
| RefSeq Size | 5608 bp |
| RefSeq ORF | 2028 bp |
| Locus ID | 64398 |
| UniProt ID | Q8N3R9 |
| Domains | SH3, PDZ, L27, Guanylate_kin, GuKc |
| Protein Families | Druggable Genome |
| Protein Pathways | Tight junction |
| MW | 77.3 kDa |
| Gene Summary | This gene encodes a member of the p55-like subfamily of the membrane-associated guanylate kinase (MAGUK) gene superfamily. The encoded protein participates in the polarization of differentiating cells, has been shown to regulate myelinating Schwann cells (PMID: 20237282), and is one of the components of the Crumbs complex in the retina. Mice which express lower levels of the orthologous protein have retinal degeneration and impaired vision (PMID: 22114289). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224752L1 | Lenti ORF clone of Human membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5) (MPP5), Myc-DDK-tagged |
CNY 7,344.00 |
|
| RC224752L2 | Lenti ORF clone of Human membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5) (MPP5), mGFP tagged |
CNY 7,700.00 |
|
| RC224752L3 | Lenti ORF clone of Human membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5) (MPP5), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC224752L4 | Lenti ORF clone of Human membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5) (MPP5), mGFP tagged |
CNY 7,700.00 |
|
| RG224752 | MPP5 (tGFP-tagged) - Human membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5) (MPP5) |
CNY 6,544.00 |
|
| SC112491 | MPP5 (untagged)-Human membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5) (MPP5) |
CNY 3,752.00 |
|
| SC318145 | MPP5 (untagged)-Human membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5) (MPP5) |
CNY 7,220.00 |
