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PHKA1 (NM_001122670) Human Untagged Clone
CAT＃: SC319028
PHKA1 (untagged)-Human phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant 2
CNY 10,450.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PHKA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC319028 representing NM_001122670.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGGAGCCGGAGTAACTCCGGGGTCCGGCTGGACGGCTACGCTCGACTGGTGCAACAGACCATCCTG TGCCATCAGAATCCAGTGACTGGCTTGCTTCCAGCCAGCTATGATCAGAAAGATGCTTGGGTCCGAGAT AATGTGTACAGCATCTTGGCTGTGTGGGGTTTGGGCCTGGCCTATCGGAAGAATGCAGACCGGGATGAG GATAAGGCAAAGGCCTATGAATTGGAGCAGAGTGTAGTGAAGCTGATGAGAGGACTACTGCACTGCATG ATCAGACAGGTGGATAAAGTAGAATCCTTCAAATATAGTCAGAGTACTAAGGATAGCCTCCATGCAAAG TACAACACCAAAACCTGTGCCACTGTAGTGGGTGATGATCAATGGGGACACCTGCAGTTGGATGCTACC TCTGTGTACCTGCTCTTCTTAGCCCAAATGACTGCCTCAGGACTCCATATCATCCACAGCCTAGATGAA GTCAATTTCATACAGAACCTTGTGTTTTACATTGAAGCTGCATATAAAACTGCTGACTTCGGGATATGG GAACGTGGAGACAAGACCAACCAAGGGATCTCAGAGTTGAATGCCAGTTCAGTTGGAATGGCAAAGGCA GCCCTGGAAGCATTAGATGAACTGGATCTGTTTGGTGTGAAAGGTGGGCCTCAATCAGTTATCCATGTC CTGGCTGATGAAGTACAGCACTGCCAGTCTATCCTAAATTCACTACTGCCCCGTGCTTCAACATCAAAA GAGGTTGATGCTAGTCTACTCTCAGTGGTTTCCTTCCCTGCCTTTGCAGTAGAGGATAGCCAGTTGGTG GAGCTCACAAAACAGGAAATCATCACCAAGCTTCAGGGTCGTTATGGTTGCTGTCGCTTTCTACGAGAT GGATATAAAACTCCTAAAGAGGATCCCAATCGTCTGTACTATGAACCAGCTGAGCTGAAGCTATTTGAA AACATTGAGTGTGAATGGCCATTGTTCTGGACATACTTTATTCTTGATGGGGTCTTCAGTGGCAATGCA GAACAGGTTCAAGAATATAAAGAGGCTCTTGAAGCAGTCCTCATCAAGGGCAAAAATGGAGTCCCACTT CTGCCAGAGCTGTACAGTGTTCCTCCTGACAGGGTCGATGAAGAATATCAGAATCCTCACACTGTGGAC CGAGTCCCCATGGGGAAATTGCCTCACATGTGGGGTCAGTCTCTATACATTTTAGGAAGCTTGATGGCA GAGGGATTTTTAGCCCCTGGAGAAATTGATCCCCTGAATCGCAGGTTTTCTACTGTACCGAAGCCCGAT GTTGTGGTTCAAGTCTCCATTCTAGCTGAAACAGAAGAAATCAAGACCATTTTGAAGGACAAGGGAATT TACGTGGAGACCATTGCTGAGGTATACCCCATCAGAGTACAACCAGCTCGTATTCTCAGCCACATTTAT TCCAGCCTAGGATGCAACAATAGAATGAAACTCAGTGGACGACCCTACAGACACATGGGAGTGCTTGGA ACTTCAAAACTCTATGACATTCGGAAAACTATCTTTACTTTCACTCCACAGTTTATAGACCAGCAACAG TTCTACCTGGCTCTGGACAACAAGATGATAGTGGAAATGCTTAGAACAGACCTCTCCTACCTCTGTAGC CGCTGGCGGATGACAGGCCAGCCCACCATCACCTTCCCCATCTCACACAGCATGCTTGATGAAGATGGA ACAAGCTTGAATTCAAGTATCCTGGCAGCACTCCGAAAAATGCAAGATGGGTATTTTGGTGGGGCAAGG GTTCAAACAGGTAAATTGTCAGAGTTTTTGACAACATCTTGTTGCACACACTTGAGCTTCATGGACCCT GGACCTGAGGGTAAGCTGTACAGTGAAGATTATGATGACAACTATGATTACCTGGAATCTGGCAACTGG ATGAATGATTATGATTCAACCAGTCATGCTCGCTGTGGTGATGAAGTTGCTCGTTATTTAGATCACCTT TTGGCGCACACTGCTCCCCATCCTAAACTAGCCCCTACCTCACAGAAGGGAGGGCTAGATCGGTTCCAA GCTGCTGTGCAAACAACCTGCGACTTAATGTCCTTGGTGACCAAGGCCAAGGAACTGCATGTACAGAAT GTTCACATGTATCTTCCTACGAAGTTATTTCAGGCTTCCCGGCCTTCATTCAACTTACTTGATTCACCT CATCCCCGACAGGAGAACCAGGTTCCCTCTGTTCGTGTAGAAATACATCTTCCTAGAGACCAGTCTGGG GAGGTGGACTTTAAAGCACTGGTTTTACAGTTGAAGGAGACCTCAAGCTTACAGGAACAAGCTGATATC CTCTATATGCTGTATACTATGAAAGGACCTGACTGGAACACTGAATTGTATAATGAACGGAGTGCTACA GTGAGAGAGCTTCTTACCGAGCTGTATGGCAAAGTGGGAGAAATTCGTCACTGGGGCCTGATCCGATAC ATTTCTGGGATCTTAAGGAAGAAAGTGGAAGCACTTGATGAGGCCTGCACAGACCTTCTCTCCCACCAG AAACATTTGACAGTAGGACTTCCTCCAGAACCTCGAGAAAAGACTATCTCTGCACCTCTGCCCTATGAG GCGCTCACTCAGCTGATAGATGAAGCCAGTGAAGGGGATATGAGCATTTCAATCCTTACACAGGAAATA ATGGTATATCTAGCCATGTATATGCGAACCCAGCCTGGCCTCTTTGCTGAAATGTTTCGACTTCGAATT GGTCTGATCATACAAGTTATGGCAACAGAACTGGCCCACTCCCTTCGATGCTCAGCTGAGGAAGCCACA GAGGGCCTGATGAATCTCAGTCCTTCGGCCATGAAGAATCTCCTGCATCACATTCTCAGCGGCAAGGAG TTTGGAGTGGAACGAAGCGTTCGTCCCACTGATTCAAATGTCAGTCCTGCTATTTCTATCCACGAGATT GGTGCTGTTGGAGCAACCAAAACAGAACGAACTGGGATCATGCAGTTAAAAAGTGAGATAAAGCAGTCA CCTGGAACCTCTATGACTCCAAGTAGTGGGTCCTTTCCTAGTGCATATGATCAGCAGTCATCTAAAGAT AGTCGTCAAGGTCAATGGCAACGCCGAAGAAGGCTGGATGGGGCACTGAATAGAGTTCCAGTTGGATTT TATCAGAAAGTATGGAAAGTTTTGCAGAAGTGTCACGGACTTTCTGTTGAAGGGTTTGTCCTTCCTTCC TCTACCACTAGAGAGATGACTCCAGGTGAGATTAAATTCTCTGTTCATGTGGAGTCTGTCCTGAATCGT GTACCTCAGCCAGAGTACCGTCAGCTGCTGGTTGAAGCCATCCTTGTCCTCACCATGCTGGCAGATATT GAAATTCATAGCATCGGAAGCATCATTGCTGTGGAAAAAATAGTGCATATTGCCAATGACTTGTTCCTT CAAGAACAGAAAACCCTTGGCGCAGATGATACCATGTTGGCAAAGGATCCCGCATCTGGCATCTGTACT CTTCTGTATGACAGTGCACCCAGTGGCAGGTTTGGCACCATGACCTACCTCTCCAAGGCAGCCGCCACC TACGTGCAGGAGTTCCTGCCCCACAGCATCTGTGCCATGCAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001122670 |
| Insert Size | 3633 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001122670.1 |
| RefSeq Size | 6138 bp |
| RefSeq ORF | 3633 bp |
| Locus ID | 5255 |
| UniProt ID | P46020 |
| Protein Families | Druggable Genome |
| Protein Pathways | Calcium signaling pathway, Insulin signaling pathway |
| MW | 135.8 kDa |
| Gene Summary | Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, and the skeletal muscle isoform is encoded by this gene. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, which are encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9D, also known as X-linked muscle glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. A pseudogene has been found on chromosome 1.[provided by RefSeq, Feb 2010] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226362 | PHKA1 (Myc-DDK-tagged)-Human phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant 2 |
CNY 8,424.00 |
|
| RC226362L1 | Lenti ORF clone of Human phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant 2, Myc-DDK-tagged |
CNY 10,824.00 |
|
| RC226362L2 | Lenti ORF clone of Human phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant 2, mGFP tagged |
CNY 7,600.00 |
|
| RC226362L3 | Lenti ORF clone of Human phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant 2, Myc-DDK-tagged |
CNY 7,600.00 |
|
| RC226362L4 | Lenti ORF clone of Human phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant 2, mGFP tagged |
CNY 7,600.00 |
|
| RG226362 | PHKA1 (tGFP-tagged) - Human phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant 2 |
CNY 6,270.00 |
