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MCM2 (NM_004526) Human Untagged Clone
CAT＃: SC319263
MCM2 (untagged)-Human minichromosome maintenance complex component 2 (MCM2)
CNY 6,632.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BM28; CCNL1; cdc19; CDCL1; D3S3194; DFNA70; MITOTIN |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_004526.2
CGCGAAACCTGGTTGTTGCTGTAGTGGCGGAGAGGATCGTGGTACTGCTATGGCGGAATC
ATCGGAATCCTTCACCATGGCATCCAGCCCGGCCCAGCGTCGGCGAGGCAATGATCCTCT CACCTCCAGCCCTGGCCGAAGCTCCCGGCGTACTGATGCCCTCACCTCCAGCCCTGGCCG TGACCTTCCACCATTTGAGGATGAGTCCGAGGGGCTCCTAGGCACAGAGGGGCCCCTGGA GGAAGAAGAGGATGGAGAGGAGCTCATTGGAGATGGCATGGAAAGGGACTACCGCGCCAT CCCAGAGCTGGACGCCTATGAGGCCGAGGGACTGGCTCTGGATGATGAGGACGTAGAGGA GCTGACGGCCAGTCAGAGGGAGGCAGCAGAGCGGGCCATGCGGCAGCGTGACCGGGAGGC TGGCCGGGGCCTGGGCCGCATGCGCCGTGGGCTCCTGTATGACAGCGATGAGGAGGACGA GGAGCGCCCTGCCCGCAAGCGCCGCCAGGTGGAGCGGGCCACGGAGGACGGCGAGGAGGA CGAGGAGATGATTGAGAGCATCGAGAACCTGGAGGATCTCAAAGGCCACTCTGTGCGCGA GTGGGTGAGCATGGCGGGCCCCCGGCTGGAGATCCACCACCGCTTCAAGAACTTCCTGCG CACTCACGTCGACAGCCACGGCCACAACGTCTTCAAGGAGCGCATCAGCGACATGTGCAA AGAGAACCGTGAGAGCCTGGTGGTGAACTATGAGGACTTGGCAGCCAGGGAGCACGTGCT GGCCTACTTCCTGCCTGAGGCACCGGCGGAGCTGCTGCAGATCTTTGATGAGGCTGCCCT GGAGGTGGTACTGGCCATGTACCCCAAGTACGACCGCATCACCAACCACATCCATGTCCG CATCTCCCACCTGCCTCTGGTGGAGGAGCTGCGCTCGCTGAGGCAGCTGCATCTGAACCA GCTGATCCGCACCAGTGGGGTGGTGACCAGCTGCACTGGCGTCCTGCCCCAGCTCAGCAT GGTCAAGTACAACTGCAACAAGTGCAATTTCGTCCTGGGTCCTTTCTGCCAGTCCCAGAA CCAGGAGGTGAAACCAGGCTCCTGTCCTGAGTGCCAGTCGGCCGGCCCCTTTGAGGTCAA CATGGAGGAGACCATCTATCAGAACTACCAGCGTATCCGAATCCAGGAGAGTCCAGGCAA AGTGGCGGCTGGCCGGCTGCCCCGCTCCAAGGACGCCATTCTCCTCGCAGATCTGGTGGA CAGCTGCAAGCCAGGAGACGAGATAGAGCTGACTGGCATCTATCACAACAACTATGATGG CTCCCTCAACACTGCCAATGGCTTCCCTGTCTTTGCCACTGTCATCCTAGCCAACCACGT GGCCAAGAAGGACAACAAGGTTGCTGTAGGGGAACTGACCGATGAAGATGTGAAGATGAT CACTAGCCTCTCCAAGGATCAGCAGATCGGAGAGAAGATCTTTGCCAGCATTGCTCCTTC CATCTATGGTCATGAAGACATCAAGAGAGGCCTGGCTCTGGCCCTGTTCGGAGGGGAGCC CAAAAACCCAGGTGGCAAGCACAAGGTACGTGGTGATATCAACGTGCTCTTGTGCGGAGA CCCTGGCACAGCGAAGTCGCAGTTTCTCAAGTATATTGAGAAAGTGTCCAGCCGAGCCAT CTTCACCACTGGCCAGGGGGCGTCGGCTGTGGGCCTCACGGCGTATGTCCAGCGGCACCC TGTCAGCAGGGAGTGGACCTTGGAGGCTGGGGCCCTGGTTCTGGCTGACCGAGGAGTGTG TCTCATTGATGAATTTGACAAGATGAATGACCAGGACAGAACCAGCATCCATGAGGCCAT GGAGCAACAGAGCATCTCCATCTCGAAGGCTGGCATCGTCACCTCCCTGCAGGCTCGCTG CACGGTCATTGCTGCCGCCAACCCCATAGGAGGGCGCTACGACCCCTCGCTGACTTTCTC TGAGAACGTGGACCTCACAGAGCCCATCATCTCACGCTTTGACATCCTGTGTGTGGTGAG GGACACCGTGGACCCAGTCCAGGACGAGATGCTGGCCCGCTTCGTGGTGGGCAGCCACGT CAGACACCACCCCAGCAACAAGGAGGAGGAGGGGCTGGCCAATGGCAGCGCTGCTGAGCC CGCCATGCCCAACACGTATGGCGTGGAGCCCCTGCCCCAGGAGGTCCTGAAGAAGTACAT CATCTACGCCAAGGAGAGGGTCCACCCGAAGCTCAACCAGATGGACCAGGACAAGGTGGC CAAGATGTACAGTGACCTGAGGAAAGAATCTATGGCGACAGGCAGCATCCCCATTACGGT GCGGCACATCGAGTCCATGATCCGCATGGCGGAGGCCCACGCGCGCATCCATCTGCGGGA CTATGTGATCGAAGACGACGTCAACATGGCCATCCGCGTGATGCTGGAGAGCTTCATAGA CACACAGAAGTTCAGCGTCATGCGCAGCATGCGCAAGACTTTTGCCCGCTACCTTTCATT CCGGCGTGACAACAATGAGCTGTTGCTCTTCATACTGAAGCAGTTAGTGGCAGAGCAGGT GACATATCAGCGCAACCGCTTTGGGGCCCAGCAGGACACTATTGAGGTCCCTGAGAAGGA CTTGGTGGATAAGGCTCGTCAGATCAACATCCACAACCTCTCTGCATTTTATGACAGTGA GCTCTTCAGGATGAACAAGTTCAGCCACGACCTGAAAAGGAAAATGATCCTGCAGCAGTT CTGAGGCCCTATGCCATCCATAAGGATTCCTTGGGATTCTGGTTTGGGGTGGTCAGTGCC CTCTGTGCTTTATGGACACAAAACCAGAGCACTTGATGAACTCGGGGTACTAGGGTCAGG GCTTATAGCAGGATGTCTGGCTGCACCTGGCATGACTGTTTGTTTCTCCAAGCCTGCTTT GTGCTTCTCACCTTTGGGTGGGATGCCTTGCCAGTGTGTCTTACTTGGTTGCTGAACATC TTGCCACCTCCGAGTGCTTTGTCTCCACTCAGTACCTTGGATCAGAGCTGCTGAGTTCAG GATGCCTGCGTGTGGTTTAGGTGTTAGCCTTCTTACATGGATGTCAGGAGAGCTGCTGCC CTCTTGGCGTGAGTTGCGTATTCAGGCTGCTTTTGCTGCCTTTGGCCAGAGAGCTGGTTG AAGATGTTTGTAATCGTTTTCAGTCTCCTGCAGGTTTCTGTGCCCCTGTGGTGGAAGAGG GCACGACAGTGCCAGCGCAGCGTTCTGGGCTCCTCAGTCGCAGGGGTGGGATGTGAGTCA TGCGGATTATCCACTCGCCACAGTTATCAGCTGCCATTGCTCCCTGTCTGTTTCCCCACT CTCTTATTTGTGCATTCGGTTTGGTTTCTGTAGTTTTAATTTTTAATAAAGTTGAATAAA ATATAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_004526 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004526.2, NP_004517.2 |
| RefSeq Size | 3453 bp |
| RefSeq ORF | 2715 bp |
| Locus ID | 4171 |
| UniProt ID | P49736 |
| Domains | MCM |
| Protein Families | Druggable Genome, Stem cell - Pluripotency, Transcription Factors |
| Protein Pathways | Cell cycle, DNA replication |
| Gene Summary | The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012] Transcript Variant: This variant (1) encodes a functional protein. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Complete In Vitro Reconstitution of Adeno-Associated Virus DNA Replication Requires the Minichromosome Maintenance Complex Proteins
,Kevin Nash, Weijun Chen, and Nicholas Muzyczka,
J. Virol., Feb 2008; 82: 1458 - 1464.
[MCM2]
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| RC202506L3 | Lenti ORF clone of Human minichromosome maintenance complex component 2 (MCM2), Myc-DDK-tagged |
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| RC202506L4 | Lenti ORF clone of Human minichromosome maintenance complex component 2 (MCM2), mGFP tagged |
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| RG202506 | MCM2 (tGFP-tagged) - Human minichromosome maintenance complex component 2 (MCM2) |
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