PEAMT (PEMT) (NM_148173) Human Untagged Clone

CAT#: SC319360

PEMT (untagged)-Human phosphatidylethanolamine N-methyltransferase (PEMT), nuclear gene encoding mitochondrial protein, transcript variant 3



  "NM_148173" in other vectors (4)

CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal Anti-PEMT Antibody
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms PEAMT; PEMPT; PEMT2; PLMT; PNMT
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for NM_148173.1 GGGGCGCGTGGCTGCAGCGGGGCCCGCGTGGTGCCTCCTGAGGCGGCCCCCGGATGAAGA
GATCTGGGAACCCGGGAGCCGAGGTAACGAACAGCTCGGTGGCAGGGCCTGACTGCTGCG
GAGGCCTCGGCAATATTGATTTTAGACAGGCAGACTTCTGCGTTATGACCCGGCTGCTGG
GCTACGTGGACCCCCTGGATCCCAGCTTTGTGGCTGCCGTCATCACCATCACCTTCAATC
CGCTCTACTGGAATGTGGTTGCACGATGGGAACACAAGACCCGCAAGCTGAGCAGGGCCT
TCGGATCCCCCTACCTGGCCTGCTACTCTCTAAGCATCACCATCCTGCTCCTGAACTTCC
TGCGCTCGCACTGCTTCACGCAGGCCATGCTGAGCCAGCCCAGGATGGAGAGCCTGGACA
CCCCCGCGGCCTACAGCCTGGGCCTCGCGCTCCTGGGACTGGGCGTCGTGCTCGTGCTCT
CCAGCTTCTTTGCACTGGGGTTCGCTGGAACTTTCCTAGGTGATTACTTCGGGATCCTCA
AGGAGGCGAGAGTGACCGTGTTCCCCTTCAACATCCTGGACAACCCCATGTACTGGGGAA
GCACAGCCAACTACCTGGGCTGGGCCATCATGCACGCCAGCCCCACGGGCCTGCTCCTGA
CGGTGCTGGTGGCCCTCACCTACATAATGGCTCTCCTATACGAAGAGCCCTTCACCGCTG
AGATCTACCGGCAGAAAGCCTCCGGGTCCCACAAGAGGAGCTGATTGAGCTGCAACAGCT
TTGCTGAAGGCCTGGCCAGCCTCCTGGCCTGCCCCAAGTGGCAGGCCCTGCGCAGGGCGA
GAATGGTGCCTGCTGCTCAGGGCTCGCCCCCGGCGTGGGCTGCCCCAGTGCCTTGGAACC
TGCTGCCTTGGGGACCCTGGACGTGCCGACATATGGCCATTGAGCTCCAACCCACACATT
CCCATTCACCAATAAAGGCACCCTGACCCCAAAAAAAAAAAAAATAAAAAAAAAAAAAAA
AAAAAAA
Restriction Sites Please inquire     
ACCN NM_148173
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_148173.1, NP_680478.1
RefSeq Size 905 bp
RefSeq ORF 600 bp
Locus ID 10400
UniProt ID Q9UBM1
Domains PEMT
Protein Families Transmembrane
Protein Pathways Glycerophospholipid metabolism, Metabolic pathways
Gene Summary Phosphatidylcholine (PC) is the most abundant mammalian phospholipid. This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. Another distinct synthetic pathway in nucleated cells converts intracellular choline to phosphatidylcholine by a three-step process. The protein isoforms encoded by this gene localize to the endoplasmic reticulum and mitochondria-associated membranes. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2012]
Transcript Variant: This variant (3) differs in the 5' UTR and uses a downstream start codon compared to variant 1. Variants 2 and 3 encode the same isoform (2). Isoform 2 localizes to mitochondria-associated membranes.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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