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E2F4 (NM_001950) Human Untagged Clone
CAT＃: SC319576
E2F4 (untagged)-Human E2F transcription factor 4, p107/p130-binding (E2F4)
CNY 3,656.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | E2F-4 |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_001950.3
GCGGCGGGCGGGCGCGATGGCGGAGGCCGGGCCACAGGCGCCGCCGCCCCCGGGCACTCC
AAGCCGGCACGAAAAGAGCCTGGGACTGCTCACCACCAAGTTCGTGTCCCTTCTGCAGGA GGCCAAGGACGGCGTGCTTGACCTCAAGCTGGCAGCTGACACCCTAGCTGTACGCCAGAA GCGGCGGATTTACGACATTACCAATGTTTTGGAAGGTATCGGGCTAATCGAGAAAAAGTC CAAGAACAGCATCCAGTGGAAGGGTGTGGGGCCTGGCTGCAATACCCGGGAGATTGCTGA CAAACTGATTGAGCTCAAGGCAGAGATCGAGGAGCTGCAGCAGCGGGAGCAAGAACTAGA CCAGCACAAGGTGTGGGTGCAGCAGAGCATCCGGAACGTCACAGAGGACGTGCAGAACAG CTGTTTGGCCTACGTCACTCATGAGGACATCTGCAGATGCTTTGCTGGAGATACCCTCTT GGCCATCCGGGCCCCATCAGGCACCAGCCTGGAGGTGCCCATCCCAGAGGGTCTCAATGG GCAGAAGAAGTACCAGATTCACCTGAAGAGTGTGAGTGGTCCCATTGAGGTTCTGCTGGT GAACAAGGAGGCATGGAGCTCACCCCCTGTGGCTGTGCCTGTGCCACCACCTGAAGATTT GCTCCAGAGCCCATCTGCTGTTTCTACACCTCCACCTCTGCCCAAGCCTGCCCTAGCCCA GTCCCAGGAAGCCTCACGTCCAAATAGTCCTCAGCTCACTCCCACTGCTGTCCCTGGCAG TGCAGAAGTCCAGGGAATGGCTGGCCCAGCAGCTGAGATCACAGTGAGTGGCGGCCCTGG GACTGATAGCAAGGACAGTGGTGAGCTCAGTTCACTCCCACTGGGCCCAACAACACTGGA CACCCGGCCACTGCAGTCTTCTGCCCTGCTGGACAGCAGCAGCAGCAGCAGCAGCAGCAG CAGCAGCAGCAGCAACAGTAACAGCAGCAGTTCGTCCGGACCCAACCCTTCTACCTCCTT TGAGCCCATCAAGGCAGACCCCACAGGTGTTTTGGAACTCCCCAAAGAGCTGTCAGAAAT CTTTGATCCCACACGAGAGTGCATGAGCTCGGAGCTGCTGGAGGAGTTGATGTCCTCAGA AGTGTTTGCCCCTCTGCTTCGTCTTTCTCCACCCCCGGGAGACCACGATTATATCTACAA CCTGGACGAGAGTGAAGGTGTCTGTGACCTCTTTGATGTGCCTGTTCTCAACCTCTGACT GACAGGGACATGCCCTGTGTGGCTGGGACCCAGACTGTCTGACCTGGGGGTTGCCTGGGG ACCTCTCCCACCCGACCCCTACAGAGCTTGAGAGCCACAGACGCCTGGCTTCTCCGGCCT CCCCTCACCGCACAGTTCTGGCCACAGCTCCCGCTCCTTCCTGGCACTTCTGTGCTCGCA GAGCAGGGGAACAGGACTCAGCCCCCATCACCGTGGAGCCAAAGTGTTTGCTTCTCCCTT TCTGCGGCCTTCGCCAGCCCAGGCTCGGCTGCCACCCAGTGGCACAGAACCGAGGAGCTG CCATTACCCCCCATAGGGGGCAGTGTCTTGTTCCTGCCAGCCTCAGTGTCTTGCTTCTGC CAGCTCCTTCCCCTAGGAGGGAAGGGTGGGGTGGAACTGGGCACATGCCAGCACCACTTC TAGCTTCCTTCGCTATCCCCCACCCCCTGACCCTCCAGCTCCTCCTGGCCCTCTCACGTG CCCACTTCTGCTGGGCCTTTAGCCCTAGAACCTGCAGGTGGTGGGGGCGGCTACCAAGAA GGAACAGAGGTCTCTGGGGAGGAGTCTGGGTGGTCCAGCCCTGATGATTGGCCCCACCTC CTGCTGCCCCATAACCCTCTCTTCATTTCGGCTTTTTCATTTACCCTCATTTAGAGCCAT TTGCAGAGATTTAGAAAGATTTACAGTAACGAATGGATTCCTATATAAAGATTATTTTTA TACTTTTTGCAGCAAAAGGAAATTGTAATATTTGTACAGTGTTCAAGTGAATAAAAACCA TGCCTAAGGCTAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_001950 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001950.3, NP_001941.2 |
| RefSeq Size | 2100 bp |
| RefSeq ORF | 1242 bp |
| Locus ID | 1874 |
| UniProt ID | Q16254 |
| Domains | E2F_TDP |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Cell cycle, TGF-beta signaling pathway |
| Gene Summary | The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Peroxisome Proliferator-Activated Receptor ß/ Cross Talks with E2F and Attenuates Mitosis in HRAS-Expressing Cells
,Bokai Zhu, Combiz Khozoie, Moses T. Bility, Christina H. Ferry, Nicholas Blazanin, Adam B. Glick, Frank J. Gonzalez, and Jeffrey M. Peters,
Mol. Cell. Biol., Jun 2012; 32: 2065 - 2082.
[E2F4]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207699 | E2F4 (Myc-DDK-tagged)-Human E2F transcription factor 4, p107/p130-binding (E2F4) |
CNY 3,656.00 |
|
| RC207699L3 | Lenti ORF clone of Human E2F transcription factor 4, p107/p130-binding (E2F4), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207699L4 | Lenti ORF clone of Human E2F transcription factor 4, p107/p130-binding (E2F4), mGFP tagged |
CNY 5,890.00 |
|
| RG207699 | E2F4 (tGFP-tagged) - Human E2F transcription factor 4, p107/p130-binding (E2F4) |
CNY 5,256.00 |
