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HPRT (HPRT1) (NM_000194) Human Untagged Clone
CAT＃: SC321340
HPRT1 (untagged)-Human hypoxanthine phosphoribosyltransferase 1 (HPRT1)
CNY 2,400.00
CNY 2,950.00
| Cited in 2 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HGPRT; HPRT |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_000194.1
CCTCCTGAGCAGTCAGCCCGCGCGCCGGCCGGCTCCGTTATGGCGACCCGCAGCCCTGGC
GTCGTGATTAGTGATGATGAACCAGGTTATGACCTTGATTTATTTTGCATACCTAATCAT TATGCTGAGGATTTGGAAAGGGTGTTTATTCCTCATGGACTAATTATGGACAGGACTGAA CGTCTTGCTCGAGATGTGATGAAGGAGATGGGAGGCCATCACATTGTAGCCCTCTGTGTG CTCAAGGGGGGCTATAAATTCTTTGCTGACCTGCTGGATTACATCAAAGCACTGAATAGA AATAGTGATAGATCCATTCCTATGACTGTAGATTTTATCAGACTGAAGAGCTATTGTAAT GACCAGTCAACAGGGGACATAAAAGTAATTGGTGGAGATGATCTCTCAACTTTAACTGGA AAGAATGTCTTGATTGTGGAAGATATAATTGACACTGGCAAAACAATGCAGACTTTGCTT TCCTTGGTCAGGCAGTATAATCCAAAGATGGTCAAGGTCGCAAGCTTGCTGGTGAAAAGG ACCCCACGAAGTGTTGGATATAAGCCAGACTTTGTTGGATTTGAAATTCCAGACAAGTTT GTTGTAGGATATGCCCTTGACTATAATGAATACTTCAGGGATTTGAATCATGTTTGTGTC ATTAGTGAAACTGGAAAAGCAAAATACAAAGCCTAAGATGAGAGTTCAAGTTGAGTTTGG AAACATCTGGAGTCCTATTGACATCGCCAGTAAAATTATCAATGTTCTAGTTCTGTGGCC ATCTGCTTAGTAGAGCTTTTTGCATGTATCTTCTAAGAATTTTATCTGTTTTGTACTTTA GAAATGTCAGTTGCTGCATTCCTAAACTGTTTATTTGCACTATGAGCCTATAGACTATCA GTTCCCTTTGGGCGGATTGTTGTTTAACTTGTAAATGAAAAAATTCTCTTAAACCACAGC ACTATTGAGTGAAACATTGAACTCATATCTGTAAGAAATAAAGAGAAGATATATTAGTTT TTTAATTGGTATTTTAATTTTTATATATGCAGGAAAGAATAGAAGTGATTGAATATTGTT AATTATACCACCGTGTGTTAGAAAAGTAAGAAGCAGTCAATTTTCACATCAAAGACAGCA TCTAAGAAGTTTTGTTCTGTCCTGGAATTATTTTAGTAGTGTTTCAGTAATGTTGACTGT ATTTTCCAACTTGTTCAAATTATTACCAGTGAATCTTTGTCAGCAGTTCCCTTTTAAATG CAAATCAATAAATTCCCAAAAATTTAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_000194 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000194.1, NP_000185.1 |
| RefSeq Size | 1331 bp |
| RefSeq ORF | 657 bp |
| Locus ID | 3251 |
| UniProt ID | P00492 |
| Domains | Pribosyltran |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| Protein Pathways | Drug metabolism - other enzymes, Metabolic pathways, Purine metabolism |
| Gene Summary | The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.[provided by RefSeq, Jun 2009] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Deficiency of the purine metabolic gene HPRT dysregulates microRNA-17 family cluster and guanine-based cellular functions: a role for EPAC in Lesch-Nyhan syndrome
,Ghiabe-Henri Guibinga, Fiona Murray, Nikki Barron, William Pandori, and Gorjan Hrustanovic,
Hum. Mol. Genet., Jul 2013; 10.1093/hmg/ddt298.
,PubMed ID 23804752
[HPRT1]
|
|
Aberrant Epigenetic Silencing Is Triggered by a Transient Reduction in Gene Expression
,null,
PLoS ONE
,PubMed ID 19279688
[HPRT1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200462 | HPRT1 (Myc-DDK-tagged)-Human hypoxanthine phosphoribosyltransferase 1 (HPRT1) |
CNY 3,600.00 |
|
| RC200462L1 | Lenti ORF clone of Human hypoxanthine phosphoribosyltransferase 1 (HPRT1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200462L2 | Lenti ORF clone of Human hypoxanthine phosphoribosyltransferase 1 (HPRT1), mGFP tagged |
CNY 5,890.00 |
|
| RC200462L3 | Lenti ORF clone of Human hypoxanthine phosphoribosyltransferase 1 (HPRT1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200462L4 | Lenti ORF clone of Human hypoxanthine phosphoribosyltransferase 1 (HPRT1), mGFP tagged |
CNY 6,000.00 |
|
| RG200462 | HPRT1 (tGFP-tagged) - Human hypoxanthine phosphoribosyltransferase 1 (HPRT1) |
CNY 5,200.00 |
