NCF1 (NM_000265) Human Untagged Clone

CAT#: SC321404

NCF1 (untagged)-Human neutrophil cytosolic factor 1 (NCF1)



  "NM_000265" in other vectors (6)

CNY 3,656.00

CNY 7,220.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Goat Polyclonal Antibody against NCF1 / p47phox
    • 100 ug

CNY 5,371.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms CGD1; NCF1A; NOXO2; p47phox; SH3PXD1A
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for NM_000265.4 CGACTTCCTCTTTCCAGTGCATTTAAGGCGCAGCCTGGAAGTGCCAGGGAGCACTGGAGG
CCACCCAGTCATGGGGGACACCTTCATCCGTCACATCGCCCTGCTGGGCTTTGAGAAGCG
CTTCGTACCCAGCCAGCACTATGTGTACATGTTCCTGGTGAAATGGCAGGACCTGTCGGA
GAAGGTGGTCTACCGGCGCTTCACCGAGATCTACGAGTTCCATAAAACCTTAAAAGAAAT
GTTCCCTATTGAGGCAGGGGCGATCAATCCAGAGAACAGGATCATCCCCCACCTCCCAGC
TCCCAAGTGGTTTGACGGGCAGCGGGCCGCCGAGAACCGCCAGGGCACACTTACCGAGTA
CTGCAGCACGCTCATGAGCCTGCCCACCAAGATCTCCCGCTGTCCCCACCTCCTCGACTT
CTTCAAGGTGCGCCCTGATGACCTCAAGCTCCCCACGGACAACCAGACAAAAAAGCCAGA
GACATACTTGATGCCCAAAGATGGCAAGAGTACCGCGACAGACATCACCGGCCCCATCAT
CCTGCAGACGTACCGCGCCATTGCCAACTACGAGAAGACCTCGGGCTCCGAGATGGCTCT
GTCCACGGGGGACGTGGTGGAGGTCGTAGAGAAGAGCGAGAGCGGTTGGTGGTTCTGTCA
GATGAAAGCAAAGCGAGGCTGGATCCCAGCGTCCTTCCTCGAGCCCCTGGACAGTCCTGA
CGAGACGGAAGACCCTGAGCCCAACTATGCAGGTGAGCCATACGTCGCCATCAAGGCCTA
CACTGCTGTGGAGGGGGACGAGGTGTCCCTGCTCGAGGGTGAAGCTGTTGAGGTCATTCA
CAAGCTCCTGGACGGCTGGTGGGTCATCAGGAAAGACGACGTCACAGGCTACTTCCCGTC
CATGTACCTGCAAAAGTCAGGGCAAGACGTGTCCCAGGCCCAACGCCAGATCAAGCGGGG
GGCGCCGCCCCGCAGGTCGTCCATCCGCAACGCGCACAGCATCCACCAGCGGTCGCGGAA
GCGCCTCAGCCAGGACGCCTATCGCCGCAACAGCGTCCGTTTTCTGCAGCAGCGACGCCG
CCAGGCGCGGCCGGGACCGCAGAGCCCCGGGAGCCCGCTCGAGGAGGAGCGGCAGACGCA
GCGCTCTAAACCGCAGCCGGCGGTGCCCCCGCGGCCGAGCGCCGACCTCATCCTGAACCG
CTGCAGCGAGAGCACCAAGCGGAAGCTGGCGTCTGCCGTCTGAGGCTGGAGCGCAGTCCC
CAGCTAGCGTCTCGGCCCTTGCCGCCCCGTGCCTGTACATACGTGTTCTATAGAGCCTGG
CGTCTGGACGCCGAGGGCAGCCCCGACCCCTGTCCAGCGCGGCTCCCGCCACCCTCAATA
AATGTTGCTTGGAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_000265
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_000265.4, NP_000256.3
RefSeq Size 1409 bp
RefSeq ORF 1173 bp
Locus ID 653361
UniProt ID P14598
Domains SH3, PX
Protein Pathways Chemokine signaling pathway, Fc gamma R-mediated phagocytosis, Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton
Gene Summary The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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