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Caldesmon (CALD1) (NM_004342) Human Untagged Clone
CAT＃: SC322530
CALD1 (untagged)-Human caldesmon 1 (CALD1), transcript variant 2
CNY 6,016.00
Product images
CNY 1,999.00
CNY 3,600.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CDM; H-CAD; HCAD; L-CAD; LCAD; NAG22 |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for SC322530
CTGTGCTCCTGCTTAAAGAAATCAGTCCTTCCTTTCCGACTTAGTCCTCGGGAAGAAGTT
TCAGACTACAAGGTATCATTGGAACATTTCAAGATCATCAAATCAAATTCCACAGGGATT GGTGACCAACCAGAAGGCTCAGACATCTGATTGCTGACCTGTCCAGACATCATCTGGTCT CCCTGAACCTGAAATCACACCATGGATGATTTTGAGCGTCGCAGAGAACTTAGAAGGCAA AAGAGGGAGGAGATGCGACTCGAAGCAGAAAGAATCGCCTACCAGAGGAATGACGATGAT GAAGAGGAGGCAGCCCGGGAACGGCGCCGCCGAGCCCGACAGGAACGGCTGCGGCAGAAG CAGGAGGAAGAATCCTTGGGACAGGTGACCGACCAGGTGGAGGTGAATGCCCAGAACAGT GTGCCTGACGAGGAGGCCAAGACAACCACCACAAACACTCAAGTGGAAGGGGATGATGAG GCCGCATTCCTGGAGCGCCTGGCTCGGCGTGAGGAAAGACGCCAAAAACGCCTTCAGGAG GCTCTGGAGCGGCAGAAGGAGTTCGACCCAACAATAACAGATGCAAGTCTGTCGCTCCCA AGCAGAAGAATGCAAAATGACACAGCAGAAAATGAAACTACCGAGAAGGAAGAAAAAAGT GAAAGTCGCCAAGAAAGATACGAGATAGAGGAAACAGAAACAGTCACCAAGTCCTACCAG AAGAATGATTGGAGGGATGCTGAAGAAAACAAGAAAGAAGACAAGGAAAAGGAGGAGGAG GAAGAGGAGAAGCCAAAGCGAGGGAGCATTGGAGAAAATCAGATCAAAGATGAAAAGATT AAAAAGGACAAAGAACCCAAAGAAGAAGTTAAGAGCTTCATGGATCGAAAGAAGGGATTT ACAGAAGTTAAGTCGCAGAATGGAGAATTCATGACCCACAAACTTAAACATACTGAGAAT ACTTTCAGCCGCCCTGGAGGGAGGGCCAGCGTGGACACCAAGGAGGCTGAGGGCGCCCCC CAGGTGGAAGCCGGCAAAAGGCTGGAGGAGCTTCGTCGTCGTCGCGGGGAGACCGAGAGC GAAGAGTTCGAGAAGCTCAAACAGAAGCAGCAGGAGGCGGCTTTGGAGCTGGAGGAACTC AAGAAAAAGAGGGAGGAGAGAAGGAAGGTCCTGGAGGAGGAAGAGCAGAGGAGGAAGCAG GAGGAAGCCGATCGAAAACTCAGAGAGGAGGAAGAGAAGAGGAGGCTAAAGGAAGAGATT GAAAGGCGAAGAGCAGAAGCTGCTGAGAAACGCCAGAAGATGCCAGAAGATGGCTTGTCA GATGACAAGAAACCATTCAAGTGTTTCACTCCTAAAGGTTCATCTCTCAAGATAGAAGAG CGAGCAGAATTTTTGAATAAGTCTGTGCAGAAAAGCAGTGGTGTCAAATCGACCCATCAA GCAGCAATAGTCTCCAAGATTGACAGCAGACTGGAGCAGTATACCAGTGCAATTGAGGGA ACAAAAAGCGCAAAACCTACAAAGCCGGCAGCCTCGGATCTTCCTGTTCCTGCTGAAGGT GTACGCAACATCAAGAGTATGTGGGAGAAAGGGAATGTGTTTTCATCCCCCACTGCAGCA GGCACACCAAATAAGGAAACTGCTGGCTTGAAGGTAGGGGTTTCTAGCCGCATCAATGAA TGGCTAACTAAAACCCCAGATGGAAACAAGTCACCTGCTCCCAAACCTTCTGACTTGAGA CCAGGAGACGTATCCAGCAAGCGGAACCTCTGGGAAAAGCAATCTGTGGATAAGGTCACT TCCCCCACTAAGGTTTGAGACAGTTCCAGAAAGAACCCAAGCTCAAGACGCAGGACGAGC TCAGTTGTAGAGGGCTAATTCGCTCTGTTTTGTATTTATGTTGATTTACTAAATTGGGTT CATTATCTTTTATTTTTCAATATCCCAGTAAACCCATGTATATTATCACTATATTTAATA ATCACAGTCTAGAGATGTTCATGGTAAAAGTACTGCCTTTGCACAGGAGCCTGTTTCTAA AGAAACCCATGCTGTGAAATAGAGACTTTTCTACTGATCATCATAACTCTGTATCTGAGC AGTGATACCAACCACATCTGAAGTCAACAGAAGATCCAAGTTTAAAATTGCCTGCGGAAT GTGTGCAGTATCTAGAAAAATGAACCGTAGTTTTTGTTTTTTTAAATACAGAAGTCATGT TGTTTCTGCACTTTATAATAAAGCATGGAAGAAATTATCTTAGTAGGCAATTGTAACACT TTTTGAAAGTAACCCATTTCAGATTTGAAATACTGCAATAATGGTTGTCTTTAAAAAAAA AAAAAGAAATGTACTGTTAAGGTATTACTTTTTTTCATGCTGATGATTCATATCTAAATT ACATTATTATGTTAGCTGACAGTGGTACTGATTTTTTAGGTTGGTTGTTTTGTGGATTTC TTTAGTAGTGATAGTAGCCTGAACCACATTTTAGATAACTCAATTATGTATGTATGTGCA TACACATATACAAACACACTAATGGTAGAATGCTTTTTTATGTGCTAGACTATTATATTT AGTAGTATGTCATTGTAACTAGCCAATATCACAGCTTTTGAAAAATTAAAAAATCACACT ATATTAATATTTCATATTTGCCAACAGAAACATGGCAGATAGGTATCAATATGTTTTCAA TGCCTGATGACCTATAAGAAGAAAGTATTGAAAAGAAGAGAGATTAGAACTGTTAGAAGG AGTTGAAATTTTCTAAAAGACATAGTATTTAGTTTATAATTAAATGCATTCTTGAAGTCC AGTGTGAATTTTATTAATGCTATCATCTCGACCAAGCTCAAAGCCTACTTATTAGAAACA ATGAAGTTCACAATAGGTCATAAGGTCTCTTCCTTTTCTAAAATTGAAAGACAAGAAATT TAGTGCCAATATTGTACAGACAGAAATTCCATGTATGAGTCTCAACAAAGACTACCTTTG GCTAAATGTCTAGAAGCAGAGAAGTAAAGTGAGCAAAATCCAGTGTTGAGGAGTCATGAC AGTACTTTGATCTTTATATACTCTGAAGCATTTCTTCAAACTTTTCTACTTTTATTTGTC ATTGATACCTGTAGTAAGTTGACAATGTGGTGAAATTTCAAAATTATATGTAACTTCTAC TAGTTTTACTTTCTCCCCCAAGTCTTTTTTAACTCATGATTTTTACACACACAATCCAGA ACTTATTATATAGCCTCTAAGTCTTTATTCTTCACAGTAGATAATGAAAGAGTCCTCCAG TGTCTTGGCAAAATGTTCTAGTATAGCTGGATACATACAGTGGAGTTCTATAAACTCATA CCTCAGTGGACTTAACCAAAATTGTGTTAGTCTCAATTCCTACCACACTGAGGGAGCCTC CCAAATAACTATTTTCTTATCTGCAGTATTCCTCCAGAAGAGCTAACCAGGGCAGGGCTG GCATGAGAAGTGACATCTGCGTTACAAAGTCTATCTTCCTCATAAGTCTGTAAAGAGCAA TTGAATCTTCTAGCTTTAGCAAACCTAAGCCAAAGGAAGGAAAGCCACGAAGAATGCAGA AGTCAAACCCTCATGACAAAGTAGGCACAAGTCTACAATAAGCTAAATCAGAATTTACAA ATACAAGTGTCCCAGGTAGCATTGACTCCCGTCATTGGAGTGAAATGGATCAAAGTTTGA ATTAAGGCCTATGGTAAGGTAACATTGCTTTGTTGTACTTTTGAACAAGAGCTCCTCCTG ATCACTATTACATATTTTTCTAGAAAATCTAAAGTTCAGAAGAGAATGTATCACTGCTGA CTTTTATTCCAATATTTGGATGGAGTAAGTTTTAGGGTAGAATTTTGTTCAGTTTGGATT TAATCTTTTGAAAAGTAAATTCCTTGTTTACTGGTTTGACTATAATTCTCTGTTATCTTT ACGAGGTAAAACTGCAAGCTGACTAGCATGTTCTGTGAATCTGCCATTCCTAAAAATTTT ATAAACACTTGATACTTTTCACTGATAATGGATCGCTCCAATAAACATATATTGTGAAAA TGCATCCACAATAAATGGAATTCCTTCCTGCAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_004342 |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004342.5, NP_004333.1 |
| RefSeq Size | 4468 bp |
| RefSeq ORF | 1617 bp |
| Locus ID | 800 |
| UniProt ID | Q05682 |
| Domains | Caldesmon |
| Protein Pathways | Vascular smooth muscle contraction |
| Gene Summary | This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) uses an alternate in-frame splice site and lacks an alternate in-frame exon in the central coding region, compared to variant 1. It is mainly expressed in non-muscle tissues or cells. The resulting isoform (2, also known as WI-38 l-CaD II) lacks an internal region, compared to isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204489 | CALD1 (Myc-DDK-tagged)-Human caldesmon 1 (CALD1), transcript variant 2 |
CNY 6,408.00 |
|
| RC204489L3 | Lenti-ORF clone of CALD1 (Myc-DDK-tagged)-Human caldesmon 1 (CALD1), transcript variant 2 |
CNY 6,460.00 |
|
| RC204489L4 | Lenti-ORF clone of CALD1 (mGFP-tagged)-Human caldesmon 1 (CALD1), transcript variant 2 |
CNY 6,460.00 |
|
| RG204489 | CALD1 (tGFP-tagged) - Human caldesmon 1 (CALD1), transcript variant 2 |
CNY 8,008.00 |
|
| SC108996 | CALD1 (untagged)-Human caldesmon 1 (CALD1), transcript variant 2 |
CNY 6,016.00 |
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