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DRAK1 (STK17A) (NM_004760) Human Untagged Clone
CAT＃: SC323411
STK17A (untagged)-Kinase deficient mutant (K90M) of Human serine/threonine kinase 17a (STK17A)
CNY 5,488.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DRAK1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323411 sequence for NM_004760 edited (data generated by NextGen Sequencing)
ATGATCCCTTTGGAGAAGCCAGGCAGCGGCGGCTCCTCCCCAGGCGCCACCTCAGGCTCG GGCCGGGCAGGCCGGGGTCTGAGCGGGCCGTGCCGGCCGCCGCCGCCGCCCCAGGCCCGC GGGCTGCTGACAGAGATACGCGCCGTGGTGCGCACCGAGCCCTTCCAGGACGGCTACAGC CTGTGCCCGGGCCGGGAGCTGGGCAGGGGGAAATTTGCAGTGGTGAGAAAATGTATAAAG AAAGATTCTGGGAAAGAATTTGCTGCAATGTTCATGAGAAAAAGAAGAAAAGGCCAAGAT TGTCGGATGGAAATAATTCATGAGATTGCTGTACTTGAACTAGCACAAGACAATCCTTGG GTCATTAATTTACATGAAGTTTATGAGACTGCATCAGAAATGATCTTAGTTCTGGAATAT GCTGCTGGGGGTGAAATCTTTGACCAGTGTGTTGCAGACAGAGAAGAAGCCTTTAAAGAA AAAGATGTTCAAAGACTTATGCGACAGATTTTAGAAGGTGTTCACTTTTTACACACTCGT GATGTAGTTCATCTTGATTTGAAGCCTCAGAATATTCTGTTGACAAGTGAATCTCCATTG GGTGACATTAAGATTGTTGATTTTGGCCTTTCAAGAATATTGAAGAACAGTGAAGAGCTC CGAGAAATTATGGGTACCCCTGAATATGTGGCTCCTGAAATTCTTAGTTATGATCCTATA AGCATGGCAACAGATATGTGGAGCATTGGAGTGTTAACATATGTCATGCTTACAGGAATA TCACCTTTCTTAGGCAATGATAAACAAGAAACATTCTTAAACATCTCACAGATGAATTTA AGTTATTCTGAGGAAGAATTTGATGTTTTGTCTGAGTCGGCTGTTGATTTCATCAGGACA CTTTTAGTTAAGAAACCTGAAGATCGAGCCACTGCTGAAGAATGTCTAAAGCACCCCTGG TTGACACAGAGCAGTATTCAAGAGCCTTCTTTCAGGATGGAAAAGGCACTAGAAGAAGCA AATGCCCTCCAAGAAGGTCATTCTGTGCCTGAAATTAATTCGGATACCGACAAATCAGAA ACCGAGGAATCCATTGTAACCGAAGAGTTAATTGTAGTTACTTCATATACTCTAGGACAA TGCAGACAGTCTGAAAAAGAGAAAATGGAGCAAAAGGCCATTTCCAAACGATTTAAATTT GAGGAACCTTTGCTACAAGAAATTCCAGGAGAATTTATCTACTGA Clone variation with respect to NM_004760.2 269 a=>t;1084 a=>g >OriGene 5' read for mutant NM_004760 unedited
ACCGCCGTTGTAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGCTGGGGAGAG CGGGTGTTTGAAGGCTCCGCGGACCGGCACTAGGAGCCGGGGGCGGGTCCGTGACCCTCCGGCTGCTCGG AGCGAACAGGCGGCCAGGAAAGAAGCGGGCCTGAACACCATGATCCCTTTGGAGAAGCCAGGCAGCGGCG GCTCCTCCCCAGGCGCCACCTCAGGCTCGGGCCGGGCAGGCCGGGGTCTGAGCGGGCCGTGCCGGCCGCC GCCGCCGCCCCAGGCCCGCGGGCTGCTGACAGAGATACGCGCCGTGGTGCGCACCGAGCCCTTCCAGGAC GGCTACCAGCCTGGTGCCCGGGCCGGGAGCTGGGCAGGGGGAAATTTGCAGTGGGTGGAGAAAAGGTTAA AGAAGGATCTGGGAAAGAATTTGCTGCAATGTTCATGAGAAAAGAAGAAAAGGCAAGATGGTCGGATGGA AATAATCATAAAATGCTTTACTTGAACTAGACAGGACATCCTTGGGTCCTTAATTTACCTGAAGTTTTTA GACTGACTACAGAATGTTCTTAGTCTGGATAGCTGTCTGGCGTGAATCTTGACCATGGTGTCAACCAGAG AAAGCCTTAGTAAGAGTTCAAGACTTTGCCAGATTGAAGGTCACTTACACCCGATGATTACTAGTGAGCC GGAATCCCGTGACTGATCCTTGGACTTAATGAATAGCTCGAGAATGACTTAAAAATCTAGGATGGAACTG GACTAGCTGAAACTCCGATGAGACCAACACTCGCCACCAG >SC323411 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CCCTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGCTGGGGAGAGCGGGTG TTTGAAGGCTCCGCGGACCGGCACTAGGAGCCGGGGGCGGGTCCGTGACCCTCCGGCTGCTCGGAGCGAA CAGGCGGCCAGGAAAGAAGCGGGCCTGAACACCATGATCCCTTTG |
| Restriction Sites | Please inquire |
| ACCN | NM_004760 |
| Insert Size | 3180 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004760.1, NP_004751.1 |
| RefSeq Size | 2641 bp |
| RefSeq ORF | 1245 bp |
| Locus ID | 9263 |
| UniProt ID | Q9UEE5 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene is a member of the DAP kinase-related apoptosis-inducing protein kinase family and encodes an autophosphorylated nuclear protein with a protein kinase domain. The protein has apoptosis-inducing activity. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Serine/Threonine Kinase 17A Is a Novel p53 Target Gene and Modulator of Cisplatin Toxicity and Reactive Oxygen Species in Testicular Cancer Cells *
,null,
The Journal of Biological Chemistry
,PubMed ID 21489989
[STK17A]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208265 | STK17A (Myc-DDK-tagged)-Human serine/threonine kinase 17a (STK17A) |
CNY 5,488.00 |
|
| RC208265L1 | Lenti ORF clone of Human serine/threonine kinase 17a (STK17A), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC208265L2 | Lenti ORF clone of Human serine/threonine kinase 17a (STK17A), mGFP tagged |
CNY 5,890.00 |
|
| RC208265L3 | Lenti ORF clone of Human serine/threonine kinase 17a (STK17A), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208265L4 | Lenti ORF clone of Human serine/threonine kinase 17a (STK17A), mGFP tagged |
CNY 7,888.00 |
|
| RG208265 | STK17A (tGFP-tagged) - Human serine/threonine kinase 17a (STK17A) |
CNY 7,088.00 |
|
| SC117160 | STK17A (untagged)-Human serine/threonine kinase 17a (STK17A) |
CNY 5,488.00 |
