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CLK2 (NM_003993) Human Untagged Clone
CAT＃: SC323449
CLK2 (untagged)-Kinase deficient mutant (K192M) of Human CDC-like kinase 2 (CLK2)
CNY 3,656.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323449 sequence for NM_003993 edited (data generated by NextGen Sequencing)
ATGCCGCATCCTCGAAGGTACCACTCCTCAGAGCGAGGCAGCCGGGGGAGTTACCGTGAA CACTATCGGAGCCGAAAGCATAAGCGACGAAGAAGTCGCTCCTGGTCAAGTAGTAGTGAC CGGACACGACGGCGTCGGCGAGAGGACAGCTACCATGTCCGTTCTCGAAGCAGTTATGAT GATCGTTCGTCCGACCGGAGGGTGTATGACCGGCGATACTGTGGCAGCTACAGACGCAAC GATTATAGCCGGGATCGGGGAGATGCCTACTATGACACAGACTATCGGCATTCCTATGAA TATCAGCGGGAGAACAGCAGTTACCGCAGCCAGCGCAGCAGCCGGAGGAAGCACAGACGG CGGAGGAGGCGCAGCCGGACATTTAGCCGCTCATCTTCGCACAGCAGCCGGAGAGCCAAG AGTGTAGAGGACGACGCTGAGGGCCACCTCATCTACCACGTCGGGGACTGGCTACAAGAG CGATATGAAATCGTTAGCACCTTAGGAGAGGGGACCTTCGGCCGAGTTGTACAATGTGTT GACCATCGCAGGGGTGGGGCTCGAGTTGCCCTGATGATCATTAAGAATGTGGAGAAGTAC AAGGAAGCAGCTCGACTTGAGATCAACGTGCTAGAGAAAATCAATGAGAAAGACCCTGAC AACAAGAACCTCTGTGTCCAGATGTTTGACTGGTTTGACTACCATGGCCACATGTGTATC TCCTTTGAGCTTCTGGGCCTTAGCACCTTCGATTTCCTCAAAGACAACAACTACCTGCCC TACCCCATCCACCAAGTGCGCCACATGGCCTTCCAGCTGTGCCAGGCTGTCAAGTTCCTC CATGATAACAAGCTGACACATACAGACCTCAAGCCTGAAAATATTCTGTTTGTGAATTCA GACTATGAGCTCACCTACAACCTAGAGAAGAAGCGAGATGAGCGCAGTGTGAAGAGCACA GCTGTGCGGGTGGTAGACTTTGGCAGTGCCACCTTTGACCATGAGCACCATAGCACCATT GTCTCCACTCGCCATTACCGAGCACCAGAAGTCATCCTTGAGTTGGGCTGGTCACAGCCT TGTGATGTGTGGAGTATAGGCTGCATCATCTTTGAATACTATGTGGGATTCACCCTCTTC CAGACCCATGACAACAGAGAGCATCTAGCCATGATGGAAAGGATCTTGGGTCCTATCCCT TCCCGGATGATCCGAAAGACAAGAAAGCAGAAATATTTTTACCGGGGTCGCCTGGATTGG GATGAGAACACATCAGCTGGGCGCTATGTTCGTGAGAACTGCAAACCGCTGCGGCGGTAT CTGACCTCAGAGGCAGAGGAACACCACCAGCTCTTCGATCTGATTGAAAGCATGCTAGAG TATGAACCAGCTAAGCGGCTGACCTTGGGTGAAGCCCTTCAGCATCCTTTCTTCGCCCGC CTTCGGGCTGAGCCGCCCAACAAGTTGTGGGACTCCAGTCGGGATATCAGTCGGTGA Clone variation with respect to NM_003993.2 575 a=>t >OriGene 5' read for mutant NM_003993 unedited
CCCGCCCGCAACAGCAAAGGGCGGTAGGCGCTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGT GAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGGAGAGGCCAGA GCCGGAGACCGAGCTGGGATCGGGCCCCGGGCGGGGGCGGTGCGAGCGGCGCCAAGCAGATCTTAGGGGC GGGGACGGAGCCGGGGCGGGCGGGACTGAAGCGGAGCCCGGGAACGGGGCGGGAGGTCCCAGGGTCCCCG GGTTGGGGGGGGTGGAGCAGGTTTAGGAATAGCAGACAGCAAAACCTTACAAGCCCAACGGATGAATTAT AGACGAAAAAAAAACATAGCAAAAAATCCCAGGCAAAAAAATAAACAAAAAACAAGGGGGAGA >SC323449 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TTYGAGKKGTTRRACTTAGAGAGGGGACTTCGGCCGAGTTGTACAATGTGTTGACCATCGCAGCCTCTCC YSYCAACTTACAGGGGTGGGGCTCGAGTTGCCCTGATGATCATTAAGAATGTGGAGAAGTACAAGGAAGC AGCTCGACTTGAGATCAACGTGCTAGAGAAAATCAATGAGAAAGACCCTGACAACAAGAACCTCTGTGTC CAGATGTTTGACTGGTTTGACTACCATGGCCACATGTGTATCTCC |
| Restriction Sites | Please inquire |
| ACCN | NM_003993 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003993.2, NP_003984.2 |
| RefSeq Size | 2175 bp |
| RefSeq ORF | 1497 bp |
| Locus ID | 1196 |
| UniProt ID | P49760 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene encodes a dual specificity protein kinase that phosphorylates serine/threonine and tyrosine-containing substrates. Activity of this protein regulates serine- and arginine-rich (SR) proteins of the spliceosomal complex, thereby influencing alternative transcript splicing. Chromosomal translocations have been characterized between this locus and the PAFAH1B3 (platelet-activating factor acetylhydrolase 1b, catalytic subunit 3 (29kDa)) gene on chromosome 19, resulting in the production of a fusion protein. Note that this gene is distinct from the TELO2 gene (GeneID:9894), which shares the CLK2 alias, but encodes a protein that is involved in telomere length regulation. There is a pseudogene for this gene on chromosome 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014] Transcript Variant: This variant (2) uses an alternate in-frame splice site, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208778 | CLK2 (Myc-DDK-tagged)-Human CDC-like kinase 2 (CLK2) |
CNY 3,656.00 |
|
| RC208778L1 | Lenti ORF clone of Human CDC-like kinase 2 (CLK2), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC208778L2 | Lenti ORF clone of Human CDC-like kinase 2 (CLK2), mGFP tagged |
CNY 5,890.00 |
|
| RC208778L3 | Lenti ORF clone of Human CDC-like kinase 2 (CLK2), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC208778L4 | Lenti ORF clone of Human CDC-like kinase 2 (CLK2), mGFP tagged |
CNY 6,056.00 |
|
| RG208778 | CLK2 (tGFP-tagged) - Human CDC-like kinase 2 (CLK2) |
CNY 5,256.00 |
|
| SC128171 | CLK2 (untagged)-Human CDC-like kinase 2 (CLK2) |
CNY 3,656.00 |
