ACK1 (TNK2) (NM_005781) Human Untagged Clone
CAT#: SC323475
TNK2 (untagged)-Kinase deficient mutant (K158M) of Human tyrosine kinase, non-receptor, 2 (TNK2), transcript variant 1
CNY 7,232.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | ACK; ACK-1; ACK1; p21cdc42Hs |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_005781, the custom clone sequence may differ by one or more nucleotides
ATGCAGCCAGAGGAGGGCACAGGCTGGCTGCTGGAGCTGCTGTCCGAGGTGCAGCTGCAACAGTACTTCC TGCGGCTCCGAGATGACCTCAACGTCACCCGCCTGTCCCACTTTGAGTACGTCAAGAATGAGGACCTGGA GAAGATCGGCATGGGTCGGCCTGGCCAGCGGCGGCTGTGGGAGGCTGTGAAGAGGAGGAAGGCCTTGTGC AAACGCAAGTCGTGGATGAGTAAGGTGTTCAGTGGAAAGCGACTGGAGGCTGAGTTCCCACCTCATCACT CTCAGAGCACCTTCCGGAAGACCTCGCCCGCCCCTGGGGGCCCAGCAGGGGAGGGGCCCCTGCAGAGCCT CACCTGCCTCATTGGGGAGAAGGACCTGCGCCTCCTGGAGAAGCTGGGTGATGGTTCCTTTGGCGTGGTG CGCAGGGGCGAGTGGGACGCGCCCTCAGGGAAGACGGTGAGTGTGGCTGTGAAGTGCCTGAAGCCCGATG TCCTGAGCCAGCCAGAAGCCATGGACGACTTCATCCGGGAGGTCAATGCCATGCACTCGCTCGACCACCG AAACCTCATCCGCCTCTACGGGGTGGTGCTCACGCCGCCCATGAAGATGGTGACAGAGCTGGCACCTCTG GGATCGTTGTTGGACCGGCTACGTAAGCACCAGGGCCACTTCCTCCTGGGGACTCTGAGCCGCTACGCTG TGCAGGTGGCTGAGGGCATGGGCTACCTGGAGTCCAAGCGCTTTATTCACCGTGACCTGGCTGCCCGCAA TCTGCTGTTGGCTACCCGCGACCTGGTCAAGATCGGGGACTTTGGGCTGATGCGAGCACTACCTCAGAAT GACGACCATTACGTCATGCAGGAACATCGCAAGGTGCCCTTCGCCTGGTGTGCCCCCGAGAGCCTGAAGA CACGCACCTTCTCCCATGCCAGCGACACCTGGATGTTCGGGGTGACACTGTGGGAAATGTTCACCTACGG CCAGGAGCCCTGGATCGGCCTCAACGGCAGTCAGATCCTGCATAAGATCGACAAGGAGGGGGAGCGGCTG CCCCGGCCCGAGGACTGTCCCCAGGACATCTACAACGTCATGGTCCAGTGCTGGGCTCACAAGCCAGAGG ACAGACCCACGTTTGTGGCCCTGCGGGACTTCCTGCTGGAGGCCCAGCCCACAGACATGCGGGCCCTTCA GGACTTTGAGGAACCGGACAAGCTGCACATCCAGATGAATGATGTCATCACCGTCATCGAGGGAAGGGCC GAGAACTACTGGTGGCGTGGCCAGAACACACGGACGCTGTGTGTGGGGCCCTTCCCTCGCAACGTGGTGA CCTCCGTGGCCGGCCTGTCGGCCCAGGACATCAGCCAGCCCCTGCAGAACAGCTTCATCCACACAGGGCA TGGCGACAGTGACCCCCGCCACTGCTGGGGCTTCCCGGACAGGATTGACGAACTGTATCTGGGAAACCCC ATGGACCCCCCCGACCTCCTGAGCGTGGAACTGAGCACCTCCCGGCCCCCCCAGCATCTAGGAGGGGTGA AAAAACCAACCTATGACCCTGTGAGCGAGGACCAAGACCCCTTGTCCAGCGACTTCAAGAGGCTGGGCCT GCGGAAGCCAGGCCTGCCCCGAGGGCTGTGGCTGGCGAAGCCCTCGGCGCGGGTGCCGGGCACCAAGGCC AGCCGAGGCAGCGGGGCTGAGGTCACGCTCATCGACTTCGGTGAGGAGCCCGTGGTCCCGGCCCTACGGC CCTGCGCGCCCTCCCTGGCGCAGCTGGCCATGGACGCCTGCTCCCTGCTGGACGAGACCCCGCCTCAGAG CCCCACGCGGGCACTGCCCCGGCCCCTGCACCCCACGCCTGTGGTGGACTGGGACGCACGCCCGCTGCCC CCCCCGCCCGCCTATGACGACGTGGCCCAGGATGAGGATGACTTTGAGATCTGCTCCATCAACAGCACCC TCGTGGGCGCGGGGGTCCCTGCCGGGCCCAGCCAGGGCCAGACCAACTACGCCTTTGTGCCTGAGCAGGC GCGGCCGCCCCCTCCCCTGGAGGACAACCTGTTCCTCCCGCCCCAGGGTGGGGGCAAGCCGCCCAGCTCC GCACAGACCGCAGAGATCTTCCAGGCGCTACAGCAGGAGTGCATGAGGCAACTGCAGGCTCCGGCCGGCT CCCCGGCCCCCTCTCCCAGCCCGGGGGGTGACGACAAGCCCCAGGTGCCTCCTCGGGTACCCATCCCCCC TCGGCCCACGCGCCCACACGTCCAGCTGTCTCCAGCCCCCCCGGGCGAGGAGGAGACCAGCCAGTGGCCT GGACCTGCTTCCCCTCCCCGGGTGCCTCCGCGGGAGCCCCTGTCCCCTCAAGGCTCGAGGACACCCAGCC CCCTGGTACCACCTGGCAGCTCCCCGCTGCCACCCCGGCTCTCAAGCTCACCTGGGAAGACCATGCCCAC CACCCAGAGCTTTGCCTCAGACCCCAAGTACGCCACCCCCCAGGTGATCCAGGCCCCTGGCCCGCGGGCT GGTCCCTGCATCCTGCCCATCGTCCGGGATGGCAAGAAGGTCAGCAGCACCCACTATTACTTGCTGCCCG AGCGACCATCCTACCTGGAGCGCTACCAGCGCTTCCTGCGTGAGGCCCAGAGCCCCGAGGAGCCTACCCC CCTGCCTGTGCCTCTGCTGCTGCCCCCACCCAGCACCCCAGCCCCCGCCGCCCCCACGGCCACCGTGCGG CCGATGCCCCAGGCTGCCTTGGACCCCAAGGCCAACTTCTCCACCAACAACAGCAACCCAGGGGCCCGGC CACCACCCCCGAGGGCCACTGCTCGGCTGCCACAGAGGGGCTGCCCTGGCGATGGGCCAGAGGCGGGCCG GCCAGCAGACAAGATCCAGATGGCCATGGTGCATGGGGTGACCACAGAGGAGTGCCAGGCGGCCCTGCAG TGCCACGGCTGGAGCGTGCAGAGGGCTGCCCAGTATCTGAAGGTGGAGCAGCTCTTCGGGCTGGGTCTGC GGCCCAGAGGGGAGTGCCACAAAGTGCTGGAGATGTTCGACTGGAACCTGGAGCAGGCCGGCTGCCACCT TCTGGGCTCCTGGGGCCCTGCCCACCACAAGCGCTGA >OriGene 5' read for mutant NM_005781 unedited
ACCGCCGTTGAGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCATTTAGGTG ACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCCCGGGATCGCGGAGCCGGCTG AGCGCAGGGCCGAGCTGCGGGGCAGAGGCTGGGAGGCGGCAGAATGCAGCCAGAGGAGGGCACAGGCTGG CTGCTGGAGCTGCTGTCCGAGGTGCAGCTGCAACAGTACTTCCTGCGGCTCCGAGACGACCTCAACGTCA CCCGCCTGTCCCACTTTGAGTACGTCAAGAATGAGGACCTGGAGAAGATCGGCATGGGTCGGCCTGCCAG CGCGCTGTGGGAGCTGTAAAGAGAGAAAGCCTGTGCAACCCCAATTCTTGGATGAATAAGGGTGTTAATT GGAAAGGCGACTGAGGGCTGATTTCCCCCCTCATCCTTTTCAAACCACCTTCCGAAAAACCTCCCCCCCC CTGGGGGCCCCCCCGGGGGAGGGCCCCTGGCAAACCCTCCCTGCCTTTTGGGGGAAAGGGCCCGGCCCCC CCGGGGAAACCTGGGGGGGGGTTTCTTTTGGCGGGGGGCCCGGGGGCAAAGGGACCCCCCCCCCGGGAAA AAGGGGAATTGGGCTTGTATTGCCCAAAACCCCGTTCCCAACACCCCCAAAAACCCGGGGACATTTACCG GGGGGGGTAGCCGGGCTCTTTTATCCCAGAACTTCTTCCCTTTTGGGGGTGGCCACCCCCCCAGAAGTCG CATAAACCCAAGGTTGCTCTATGCTTATTTTCATCTCCCCTCACCCTG >SC323475 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
GMTKGGCTGWGKTCTTTGGCGTGGTGCGCAGGGGCGAGTGGGACGCGCCCTCAGGGAAGACGGTGAGTGT GGCTGTGATGTGCCTGAAGCCCGATGTCCTGAGCCAGCCAGAAGCCATGGACGACTTCATCCGGGAGGTC AATGCCATGCACTCGCTCGACCACCGAAACCTCATCCGCCTCTACGGGGTGGTGCTCACGCCGCCCATGA AGATGGTGACAGAGCTGGCACCTCTGGGATCGTTGTTGGACCGGC |
Restriction Sites | Please inquire |
ACCN | NM_005781 |
Insert Size | 4100 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_005781.4, NP_005772.3 |
RefSeq Size | 4476 bp |
RefSeq ORF | 3117 bp |
Locus ID | 10188 |
UniProt ID | Q07912 |
Domains | UBA, pkinase, TyrKc, SH3, S_TKc |
Protein Families | Druggable Genome, Protein Kinase |
Gene Summary | This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) is the more frequently occurring transcript and it encodes isoform 1. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC213566 | TNK2 (Myc-DDK-tagged)-Human tyrosine kinase, non-receptor, 2 (TNK2), transcript variant 1 |
CNY 7,232.00 |
|
RC213566L1 | Lenti-ORF clone of TNK2 (Myc-DDK-tagged)-Human tyrosine kinase, non-receptor, 2 (TNK2), transcript variant 1 |
CNY 9,632.00 |
|
RC213566L2 | Lenti-ORF clone of TNK2 (mGFP-tagged)-Human tyrosine kinase, non-receptor, 2 (TNK2), transcript variant 1 |
CNY 12,260.00 |
|
RC213566L3 | Lenti-ORF clone of TNK2 (Myc-DDK-tagged)-Human tyrosine kinase, non-receptor, 2 (TNK2), transcript variant 1 |
CNY 12,260.00 |
|
RC213566L4 | Lenti-ORF clone of TNK2 (mGFP-tagged)-Human tyrosine kinase, non-receptor, 2 (TNK2), transcript variant 1 |
CNY 12,260.00 |
|
RG213566 | TNK2 (tGFP-tagged) - Human tyrosine kinase, non-receptor, 2 (TNK2), transcript variant 1 |
CNY 11,400.00 |
|
SC128079 | TNK2 (untagged)-Human tyrosine kinase, non-receptor, 2 (TNK2), transcript variant 1 |
CNY 7,232.00 |