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ZIP Kinase (DAPK3) (NM_001348) Human Untagged Clone
CAT＃: SC323479
DAPK3 (untagged)-Kinase deficient mutant (K42M) of Human death-associated protein kinase 3 (DAPK3)
CNY 3,656.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DLK; ZIP; ZIPK |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323479 sequence for NM_001348 edited (data generated by NextGen Sequencing)
ATGTCCACGTTCAGGCAGGAGGACGTGGAGGACCATTATGAGATGGGGGAGGAGCTGGGC AGCGGCCAGTTTGCGATCGTGCGGAAGTGCCGGCAGAAGGGCACGGGCAAGGAGTACGCA GCCATGTTCATCAAGAAGCGCCGCCTGTCATCCAGCCGGCGTGGGGTGAGCCGGGAGGAG ATCGAGCGGGAGGTGAACATCCTGCGGGAGATCCGGCACCCCAACATCATCACCCTGCAC GACATCTTCGAGAACAAGACGGACGTGGTCCTCATCCTGGAGCTGGTCTCTGGCGGGGAG CTCTTTGACTTCCTGGCGGAGAAGGAGTCGCTGACGGAGGACGAGGCCACCCAGTTCCTC AAGCAGATCCTGGACGGCGTTCACTACCTGCACTCTAAGCGCATCGCACACTTTGACCTG AAGCCGGAAAACATCATGCTGCTGGACAAGAACGTGCCCAACCCACGAATCAAGCTCATC GACTTCGGCATCGCGCACAAGATCGAGGCGGGGAACGAGTTCAAGAACATCTTCGGCACC CCGGAGTTTGTGGCCCCAGAGATTGTGAACTATGAGCCGCTGGGCCTGGAGGCGGACATG TGGAGCATCGGTGTCATCACCTATATCCTCCTGAGCGGTGCATCCCCGTTCCTGGGCGAG ACCAAGCAGGAGACGCTCACCAACATCTCAGCCGTGAACTACGACTTCGACGAGGAGTAC TTCAGCAACACCAGCGAGCTGGCCAAGGACTTCATTCGCCGGCTGCTCGTCAAAGATCCC AAGCGGAGAATGACCATTGCCCAGAGCCTGGAACATTCCTGGATTAAGGCGATCCGGCGG CGGAACGTGCGTGGTGAGGACAGCGGCCGCAAGCCCGAGCGGCGGCGCCTGAAGACCACG CGTCTGAAGGAGTACACCATCAAGTCGCACTCCAGCTTGCCGCCCAACAACAGCTACGCC GACTTCGAGCGCTTCTCCAAGGTGCTGGAGGAGGCGGCGGCCGCCGAGGAGGGCCTGCGC GAGCTGCAGCGCAGCCGGCGGCTCTGCCACGAGGACGTGGAGGCGCTGGCCGCCATCTAC GAGGAGAAGGAGGCCTGGTACCGCGAGGAGAGCGACAGCCTGGGCCAGGACCTGCGGAGG CTACGGCAGGAGCTGCTCAAGACCGAGGCGCTCAAGCGGCAGGCGCAGGAGGAGGCCAAG GGCGCGCTGCTGGGGACCAGCGGCCTCAAGCGCCGCTTCAGCCGCCTGGAGAACCGCTAC GAGGCGCTGGCCAAGCAAGTAGCCTCCGAGATGCGCTTCGTGCAGGACCTCGTGCGCGCC CTGGAGCAGGAGAAGCTGCAGGGCGTGGAGTGCGGGCTGCGCTAG Clone variation with respect to NM_001348.1 125 a=>t;324 a=>g >OriGene 5' read for mutant NM_001348 unedited
ACCGCCCGCTCGCAGCAAAGGGCGGTAGGCGCTGTACGGTTGGGAGGTTCTATATAAGCAGAGCTCGTTT AGTGAACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGCGCGGCGG CGCCGGCCGTATTCTCCGGGCTGCGGAGGGGTTGCCATTAGGGGACTCCTGAGGTCCTATCTCCAGGCTG CGGTGACTGCACTTTCCCTGGAGTGGAAGCTGCTGGAAGGCGGACCGGCCGCCATGTCCACGTTCAGGCA GGAGGACGTGGAGGACCATTATGAGATGGGGGAGGAGCTGGCAGCGCCCATTTTGCGATCGTGCGGGAAG TGGCCGGCAAAAGGCCACGGGCCAAGGATTCCCCACCCATGTTCCATAAAAAACCGCCACCTGGTATCAA CCCGGGCTTGGGGAAACCAGGAGGAAAATAAAGCGGGAGAAAAACCCGAGGGGGGATAAAAAAAAAAAAA ACATTGCA >SC323479 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CCCTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGCGCGGCGGCGCCGGCCGTA TTCTCCGGGCTGCGGAGGGGTTGCCATTAGGGGACTCCTGAGGTCCTATCTCCAGGCTGCGGTGACTGCA CTTTCCCTGGAGTGGAAGCTGCTGGAAGGCGGACCGGCCGCCATG |
| Restriction Sites | Please inquire |
| ACCN | NM_001348 |
| Insert Size | 1090 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001348.1, NP_001339.1 |
| RefSeq Size | 2105 bp |
| RefSeq ORF | 1365 bp |
| Locus ID | 1613 |
| UniProt ID | O43293 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Bladder cancer, Pathways in cancer |
| Gene Summary | Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the induction of apoptosis. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213427 | DAPK3 (Myc-DDK-tagged)-Human death-associated protein kinase 3 (DAPK3) |
CNY 3,656.00 |
|
| RC213427L1 | Lenti ORF clone of Human death-associated protein kinase 3 (DAPK3), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC213427L2 | Lenti ORF clone of Human death-associated protein kinase 3 (DAPK3), mGFP tagged |
CNY 5,890.00 |
|
| RC213427L3 | Lenti ORF clone of Human death-associated protein kinase 3 (DAPK3), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC213427L4 | Lenti ORF clone of Human death-associated protein kinase 3 (DAPK3), mGFP tagged |
CNY 6,056.00 |
|
| RG213427 | DAPK3 (tGFP-tagged) - Human death-associated protein kinase 3 (DAPK3) |
CNY 4,370.00 |
|
| SC119321 | DAPK3 (untagged)-Human death-associated protein kinase 3 (DAPK3) |
CNY 3,656.00 |
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