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GRK5 (NM_005308) Human Untagged Clone
CAT＃: SC323499
GRK5 (untagged)-Kinase deficient mutant (K215M) of Human G protein-coupled receptor kinase 5 (GRK5)
CNY 4,400.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | FP2025; GPRK5 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323499 sequence for NM_005308 edited (data generated by NextGen Sequencing)
ATGGAGCTGGAAAACATCGTGGCCAACACGGTCTTGCTGAAAGCCAGGGAAGGGGGCGGA GGAAAGCGCAAAGGGAAAAGCAAGAAGTGGAAAGAAATCCTGAAGTTCCCTCACATTAGC CAGTGTGAAGACCTCCGAAGGACCATAGACAGAGATTACTGCAGTTTATGTGACAAGCAG CCAATCGGGAGGCTGCTTTTCCGGCAGTTTTGTGAAACCAGGCCTGGGCTGGAGTGTTAC ATTCAGTTCCTGGACTCCGTGGCAGAATATGAAGTTACTCCAGATGAAAAACTGGGAGAG AAAGGGAAGGAAATTATGACCAAGTACCTCACCCCAAAGTCCCCTGTTTTCATAGCCCAA GTTGGCCAAGACCTGGTCTCCCAGACGGAGGAGAAGCTCCTACAGAAGCCGTGCAAAGAA CTCTTTTCTGCCTGTGCACAGTCTGTCCACGAGTACCTGAGGGGAGAACCATTCCACGAA TATCTGGACAGCATGTTTTTTGACCGCTTTCTCCAGTGGAAGTGGTTGGAAAGGCAACCG GTGACCAAAAACACTTTCAGGCAGTATCGAGTGCTAGGAAAAGGGGGCTTCGGGGAGGTC TGTGCCTGCCAGGTTCGGGCCACGGGTAAAATGTATGCCTGCATGCGCTTGGAGAAGAAG AGGATCAAAAAGAGGAAAGGGGAGTCCATGGCCCTCAATGAGAAGCAGATCCTCGAGAAG GTCAACAGTCAGTTTGTGGTCAACCTGGCCTATGCCTACGAGACCAAGGATGCACTGTGC TTGGTCCTGACCATCATGAATGGGGGTGACCTGAAGTTCCACATCTACAACATGGGCAAC CCTGGCTTCGAGGAGGAGCGGGCCTTGTTTTATGCGGCAGAGATCCTCTGCGGCTTAGAA GACCTCCACCGTGAGAACACCGTCTACCGAGATCTGAAACCTGAAAACATCCTGTTAGAT GATTATGGCCACATTAGGATCTCAGACCTGGGCTTGGCTGTGAAGATCCCCGAGGGAGAC CTGATCCGCGGCCGGGTGGGCACTGTTGGCTACATGGCTCCAGAGGTCCTGAACAACCAG AGGTACGGCCTGAGCCCCGACTACTGGGGCCTTGGCTGCCTCATCTATGAGATGATCGAG GGCCAGTCGCCGTTCCGCGGCCGCAAGGAGAAGGTGAAGCGGGAGGAGGTGGACCGCCGG GTCCTGGAGACGGAGGAGGTGTACTCCCACAAGTTCTCCGAGGAGGCCAAGTCCATCTGC AAGATGCTGCTCACGAAAGATGCGAAGCAGAGGCTGGGCTGCCAGGAGGAGGGGGCTGCA GAGGTCAAGAGACACCCCTTCTTCAGGAACATGAACTTCAAGCGCTTAGAAGCCGGGATG TTGGACCCTCCCTTCGTTCCAGACCCCCGCGCTGTGTACTGTAAGGACGTGCTGGACATC GAGCAGTTCTCCACTGTGAAGGGCGTCAATCTGGACCACACAGACGACGACTTCTACTCC AAGTTCTCCACGGGCTCTGTGTCCATCCCATGGCAAAACGAGATGATAGAAACAGAATGC TTTAAGGAGCTGAACGTGTTTGGACCTAATGGTACCCTCCCGCCAGATCTGAACAGAAAC CACCCTCCGGAACCGCCCAAGAAAGGGCTGCTCCAGAGACTCTTCAAGCGGCAGCATCAG AACAATTCCAAGAGTTCGCCCAGCTCCAAGACCAGTTTTAACCACCACATAAACTCAAAC CATGTCAGCTCGAACTCCACCGGAAGCAGCTAG Clone variation with respect to NM_005308.2 644 a=>t >OriGene 5' read for mutant NM_005308 unedited
CGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGGTAGGCAAGGCG GGCTGCTGGCTCCCCCGGCTCCGGCAGCAGCGGCGGCAGCCCGAGCAGCGGCAGCAGCAGCGGCAGCACC CCAGGCGCTGACAGCCCCGCCGGCCGGCTCCGTTGCTGACCGCCGACTGTCAATGGAGCTGGAAAACATC GTGGCCAACACGGTCTTGCTGAAAGCCAGGGAAGGGGGCGGAGGAAAGCGCAAAGGGAAAAGCAAGAAGT GGAAAGAAATCCTGAGGTTCCCTCACATTAGCCAGTGTGAAGACCTCCGAAGGACAATAGACAAGAAAAT AACCTGGCAAGTTATATGTGACCAAGCCAGCCAATCCGGGAGGGCTTGCTTTTTCCGGCAGTTTTGGTGA AACCCAGGCCTGGGGCTGGAGATGGTTACATTCAGTTCCGGACTCCCGGTGGGAAAATAATGAGGTAATT CCAAATGAAAACTGGGGAGAAAAAGGGAAGGGAATTTTTGACAGTTACCTACCCCAAGGTCCCCGTGTTT TCATAGCCAGTTTGCCCAGCCTGGGTCTCCCAAAGGGGAGAAACTCTCCTACAAACGCTGCGCAGAGATC TTTTCTGTGTGTGCGCACACTGTGTCCAAGACCCGAGGGGGAACACTTCTCCCAATATTGTCGACACGTG TTTGACGCGTTTCCACTGTAGAAGGTGGTGAGAGGCCAGTGTCACACAACACTCTTGCGCATTCATAGCT CAGAAAGGGCTCTGCGAGACTGTCGTGCCAAATTGCAACGGGAATATGACCCTCGCTTCGGAAAGAAAAC >SC323499 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
GMTGGTGACAAAACACTTTMGGCAGTATCGAGTGCTAGGAAAAGGGGGCTTCGGGGAGGTCTGTGCCTGC CAGGTTCGGGCCACGGGTAAAATGTATGCCTGCATGCGCTTGGAGAAGAAGAGGATCAAAAAGAGGAAAG GGGAGTCCATGGCCCTCAATGAGAAGCAGATCCTCGAGAAGGTCAACAGTCAGTTTGTGGTCAACCTGGC CTATGCCTACGAGACCAAGGATGCACTGTGCTTGGTCCTGACCAT |
| Restriction Sites | Please inquire |
| ACCN | NM_005308 |
| Insert Size | 2280 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005308.2, NP_005299.1 |
| RefSeq Size | 2575 bp |
| RefSeq ORF | 1773 bp |
| Locus ID | 2869 |
| UniProt ID | P34947 |
| Domains | RGS, pkinase, S_TK_X, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Chemokine signaling pathway, Endocytosis |
| Gene Summary | This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. It has also been shown to play a role in regulating the motility of polymorphonuclear leukocytes (PMNs). [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209137 | GRK5 (Myc-DDK-tagged)-Human G protein-coupled receptor kinase 5 (GRK5) |
CNY 4,392.00 |
|
| RC209137L1 | Lenti ORF clone of Human G protein-coupled receptor kinase 5 (GRK5), Myc-DDK-tagged |
CNY 6,792.00 |
|
| RC209137L2 | Lenti ORF clone of Human G protein-coupled receptor kinase 5 (GRK5), mGFP tagged |
CNY 5,890.00 |
|
| RC209137L3 | Lenti ORF clone of Human G protein-coupled receptor kinase 5 (GRK5), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209137L4 | Lenti ORF clone of Human G protein-coupled receptor kinase 5 (GRK5), mGFP tagged |
CNY 6,792.00 |
|
| RG209137 | GRK5 (tGFP-tagged) - Human G protein-coupled receptor kinase 5 (GRK5) |
CNY 5,992.00 |
|
| SC110910 | GRK5 (untagged)-Human G protein-coupled receptor kinase 5 (GRK5) |
CNY 2,950.00 |
