United States
Germany
Japan
United Kingdom
China
MLK3 (MAP3K11) (NM_002419) Human Untagged Clone
CAT＃: SC323524
MAP3K11 (untagged)-Kinase deficient mutant (K144M) of Human mitogen-activated protein kinase kinase kinase 11 (MAP3K11)
CNY 6,208.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MEKK11; MLK-3; MLK3; PTK1; SPRK |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323524 sequence for NM_002419 edited (data generated by NextGen Sequencing)
ATGGAGCCCTTGAAGAGCCTCTTCCTCAAGAGCCCTCTAGGGTCATGGAATGGCAGTGGC AGCGGGGGTGGTGGGGGCGGTGGAGGAGGCCGGCCTGAGGGGTCTCCAAAGGCAGCGGGT TATGCCAACCCGGTGTGGACAGCCCTGTTCGACTACGAGCCCAGTGGGCAGGATGAGCTG GCCCTGAGGAAGGGTGACCGTGTGGAGGTGCTGTCCCGGGACGCAGCCATCTCAGGAGAC GAGGGCTGGTGGGCGGGCCAGGTGGGTGGCCAGGTGGGCATCTTCCCGTCCAACTATGTG TCTCGGGGTGGCGGCCCGCCCCCCTGCGAGGTGGCCAGCTTCCAGGAGCTGCGGCTGGAG GAGGTGATCGGCATTGGAGGCTTTGGCAAGGTGTACAGGGGCAGCTGGCGAGGTGAGCTG GTGGCTGTGATGGCAGCTCGCCAGGACCCCGATGAGGACATCAGTGTGACAGCCGAGAGC GTTCGCCAGGAGGCCCGGCTCTTCGCCATGCTGGCACACCCCAACATCATTGCCCTCAAG GCTGTGTGCCTGGAGGAGCCCAACCTGTGCCTGGTGATGGAGTATGCAGCCGGTGGGCCC CTCAGCCGAGCTCTGGCCGGGCGGCGCGTGCCTCCCCATGTGCTGGTCAACTGGGCTGTG CAGATTGCCCGTGGGATGCACTACCTGCACTGCGAGGCCCTGGTGCCCGTCATCCACCGT GATCTCAAGTCCAACAACATTTTGCTGCTGCAGCCCATTGAGAGTGACGACATGGAGCAC AAGACCCTGAAGATCACCGACTTTGGCCTGGCCCGAGAGTGGCACAAAACCACACAAATG AGTGCCGCGGGCACCTACGCCTGGATGGCTCCTGAGGTTATCAAGGCCTCCACCTTCTCT AAGTGCAGTGACGTCTGGAGTTTTGGGGTGCTGCTGTGGGAACTGCTGACCGGGGAGGTG CCATACCGTGGCATTGACTGCCTTGCTGTGGCCTATGGCGTAGCTGTTAACAAGCTCACA CTGCCCATCCCATCCACCTGCCCCGAGCCCTTCGCACAGCTTATGGCCGACTGCTGGGCG CAGGACCCCCACCGCAGGCCCGACTTCGCCTCCATCCTGCAGCAGTTGGAGGCGCTGGAG GCACAGGTCCTACGGGAAATGCCGCGGGACTCCTTCCATTCCATGCAGGAAGGCTGGAAG CGCGAGATCCAGGGTCTCTTCGACGAGCTGCGAGCCAAGGAAAAGGAACTACTGAGCCGC GAGGAGGAGCTGACGCGAGCGGCGCGCGAGCAGCGGTCACAGGCGGAGCAGCTGCGGCGG CGCGAGCACCTGCTGGCCCAGTGGGAGCTAGAGGTGTTCGAGCGCGAGCTGACGCTGCTG CTGCAGCAGGTGGACCGCGAGCGACCGCACGTGCGCCGCCGCCGCGGGACATTCAAGCGC AGCAAGCTCCGGGCGCGCGACGGCGGCGAGCGTATCAGCATGCCACTCGACTTCAAGCAC CGCATCACCGTGCAGGCCTCACCCGGCCTTGACCGGAGGAGAAACGTCTTCGAGGTCGGG CCTGGGGATTCGCCCACCTTTCCCCGGTTCCGAGCCATCCAGTTGGAGCCTGCAGAGCCA GGCCAGGCATGGGGCCGCCAGTCCCCCCGACGTCTGGAGGACTCAAGCAATGGAGAGCGG CGAGCATGCTGGGCTTGGGGTCCCAGTTCCCCCAAGCCTGGGGAAGCCCAGAATGGGAGG AGAAGGTCCCGCATGGACGAAGCCACATGGTACCTGGATTCAGATGACTCATCCCCCTTA GGATCTCCTTCCACACCCCCAGCACTCAATGGTAACCCCCCGCGGCCTAGCCTGGAGCCC GAGGAGCCCAAGAGGCCTGTCCCCGCAGAGCGCGGTAGCAGCTCTGGGACGCCCAAGCTG ATCCAGCGGGCGCTGCTGCGCGGCACCGCCCTGCTCGCCTCGCTGGGCCTTGGCCGCGAC CTGCAGCCGCCGGGAGGCCCAGGACGCGAGCGCGGGGAGTCCCCGACAACACCCCCCACG CCAACGCCCGCGCCCTGCCCGACCGAGCCGCCCCCTTCCCCGCTCATCTGCTTCTCGCTC AAGACGCCCGACTCCCCGCCCACTCCTGCACCCCTGTTGCTGGACCTGGGTATCCCTGTG GGCCAGCGGTCAGCCAAGAGCCCCCGACGTGAGGAGGAGCCCCGCGGAGGCACTGTCTCA CCCCCACCGGGGACATCACGCTCTGCTCCTGGCACCCCAGGCACCCCACGTTCACCACCC CTGGGCCTCATCAGCCGACCTCGGCCCTCGCCCCTTCGCAGCCGCATTGATCCCTGGAGC TTTGTGTCAGCTGGGCCACGGCCTTCTCCCCTGCCATCACCACAGCCTGCACCCCGCCGA GCACCCTGGACCTTGTTCCCGGACTCAGACCCCTTCTGGGACTCCCCACCTGCCAACCCC TTCCAGGGGGGCCCCCAGGACTGCAGGGCACAGACCAAAGACATGGGTGCCCAGGCCCCG TGGGTGCCGGAAGCGGGGCCTTGA Clone variation with respect to NM_002419.3 431 a=>t;904 g=>t >OriGene 5' read for mutant NM_002419 unedited
ACGCCCGTATCCGCAAAGGGCGGTAGGCGCGTACGGTGGGAGGTTCTATATAAGCAGAAGCTCGTTTAGT GAACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGGGCCAGGAA CAGGCAGTCTCGGCCCAACTGCGGACGCTCCCTCCACCCCCTGCGCAAAAAGACCCAACCGGAGTTGAGG CGCTGCCCCTGAAGGCCCCACCTTACACTTGGCGGGGGCCGGAGCCAGGCTCCCAGGACTGCTCCAGAAC CGAGGGAAGCTCGGGTCCCTCCAAGCTAGCCATGGTGAGGCGCCGGAGGCCCCGGGGGCCCCACCCCCCC CGGCCTGACCACACTGCCCTGGGGTGCCCTCCTCCAGAGGCCCGAGATGCGGGGGGGCCGGGAAACCAAC CACTTCCTGCCTCCCCCAGAGAAGCCTTGGTTCTTGGGGCTTGAAGGGCCCAGGGCCCCGGATGGCCCAG GTTCCGGGACTAGGGCCTTGGCACCCACCGGGGGTTGGGAACCACGGCACCCCCGAAGAAGGTCCTCCAC CATCCCAAGGGCGGGCTCCGGCATGGGGCCCCTTTAGAGCCCTTCTCTCTCAAGAGCCCTAAGGTCTATG AATGGCCATTGCCACCGGGTTTTGGGGCCGTGTAGAAACCCCCCTCAGGGGTTCACAAGCGCGGGGATAC CCACACCGTGTTGACACACTGTTTTCTATAAGCCCCTGTGCACATGAGATGCTCCTCATGAAAATGACAC CTGTAGTGCTTCCCCGACCACCATCTCTGAGACAAAGCTGTTGTCGCCACATGTGTCACATGGGCCTTCC TCCACCATGTTACGATGCGCGCCCCTCGCAGTGACATCTAACTGGTGGGGAGGGTATCACTTGAGCTGCA GGTAGGCACTCCGAGATACGTGACGTCAGAGATTGCGA >SC323524 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
GCATGSGAGTGGCAGCTTCAGGAGCTGCGGCTGGAGGAGGTGATCGGCATTGGAGGCTTTGGCAAGGTGT ACAGGGGCAGCTKGCGAGGTGAGCTGGTGGCTGTGATGGCAGCTCGCCAGGACCCCGATGAGGACATCAG TGTGACAGCCGAGAGCGTTCGCCAGGAGGCCCGGCTCTTCGCCATGCTGGCACACCCCAACATCATTGCC CTCAAGGCTGTGTGCCTGGAGGAGCCCAACCTGTGCCTGGTGATG |
| Restriction Sites | Please inquire |
| ACCN | NM_002419 |
| Insert Size | 3860 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002419.3, NP_002410.1 |
| RefSeq Size | 3574 bp |
| RefSeq ORF | 2544 bp |
| Locus ID | 4296 |
| UniProt ID | Q16584 |
| Domains | pkinase, TyrKc, SH3, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | MAPK signaling pathway |
| Gene Summary | The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase contains a SH3 domain and a leucine zipper-basic motif. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of JNK signaling pathway. This kinase can directly phosphorylate, and activates IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203782 | MAP3K11 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase 11 (MAP3K11) |
CNY 6,208.00 |
|
| RC203782L1 | Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase 11 (MAP3K11), Myc-DDK-tagged |
CNY 8,608.00 |
|
| RC203782L2 | Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase 11 (MAP3K11), mGFP tagged |
CNY 5,890.00 |
|
| RC203782L3 | Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase 11 (MAP3K11), Myc-DDK-tagged |
CNY 8,608.00 |
|
| RC203782L4 | Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase 11 (MAP3K11), mGFP tagged |
CNY 8,608.00 |
|
| RG203782 | MAP3K11 (tGFP-tagged) - Human mitogen-activated protein kinase kinase kinase 11 (MAP3K11) |
CNY 7,808.00 |
|
| SC110949 | MAP3K11 (untagged)-Human mitogen-activated protein kinase kinase kinase 11 (MAP3K11) |
CNY 6,208.00 |
