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TNK1 (NM_003985) Human Untagged Clone
CAT＃: SC323530
TNK1 (untagged)-Kinase deficient mutant (K148M) of Human tyrosine kinase, non-receptor, 1 (TNK1)
CNY 4,888.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | KOS1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323530 sequence for NM_003985 edited (data generated by NextGen Sequencing)
ATGCTTCCTGAGGCTGGCTCTCTGTGGCTACTGAAGCTGCTCCGGGACATCCAGTTGGCC CAGTTTTACTGGCCCATCCTTGAGGAGCTTAATGTCACTCGGCCAGAGCACTTCGACTTT GTAAAGCCTGAGGACCTGGACGGCATTGGCATGGGCCGGCCTGCCCAGCGCAGACTGTCC GAAGCTCTGAAAAGGCTACGTTCTGGGCCTAAGTCTAAGAACTGGGTCTACAAGATCCTT GGAGGTTTTGCCCCTGAGCACAAGGAGCCCACCCTGCCCTCGGACAGCCCACGGCACCTC CCTGAGCCAGAGGGGGGCCTCAAGTGTCTGATCCCAGAGGGTGCTGTTTGCAGAGGGGAG CTGCTGGGTTCAGGCTGCTTCGGTGTGGTGCACCGAGGGCTGTGGACGCTGCCCAGTGGC AAGAGTGTCCCAGTGGCTGTCATGTCCCTCCGGGTAGGTCCCGAAGGCCCGATGGGCACA GAACTGGGGGACTTCCTGCGAGAGGTATCGGTCATGATGAACTTGGAGCACCCACACGTG CTGCGTCTGCACGGCCTTGTACTGGGCCAGCCTCTGCAGATGGTGATGGAGCTGGCGCCA CTGGGCTCCCTGCACGCGCGCCTAACGGCCCCGGCCCCGACACCCCCGCTGCTCGTGGCC CTGCTCTGCCTCTTCCTGCGGCAGCTGGCGGGAGCCATGGCGTACCTGGGGGCCCGCGGG CTGGTGCACCGAGACCTCGCTACGCGCAACCTACTGCTGGCGTCGCCGCGCACCATCAAG GTGGCTGACTTCGGGCTGGTGCGGCCTCTGGGCGGTGCCCGGGGCCGCTACGTCATGGGC GGGCCCCGCCCTATCCCCTACGCCTGGTGTGCCCCAGAGAGCCTGCGCCACGGAGCCTTC TCGTCTGCCTCGGACGTGTGGATGTTTGGGGTGACGCTGTGGGAGATGTTCTCCGGGGGC GAGGAACCCTGGGCCGGGGTCCCACCGTACCTCATCCTGCAGCGGCTGGAGGACAGAGCC CGGCTGCCTAGGCCTCCCCTCTGCTCCAGGGCCCTCTACTCCCTCGCCTTGCGCTGCTGG GCCCCCCACCCTGCCGACCGGCCTAGCTTTTCCCACCTGGAGGGGCTGCTGCAAGAGGCC GGGCCTTCGGAAGCATGTTGTGTGAGGGATGTCACAGAACCAGGCGCCCTGAGGATGGAG ACTGGTGACCCCATCACAGTCATCGAGGGCAGCCCCGACTCCACAATCTGGAAGGGCCAG AATGGTCGCACCTTCAAAGTGGGCAGCTTCCCAGCCTCGGCAGTGACGCTGGCAGATGCG GGGGGCTTGCCAGCCACCCGTCCAGTCCACAGAGGCACCCCTGCCCGGGGAGATCAACAC CCAGGAAGCATAGATGGAGACAGAAAGAAGGCAAATCTTTGGGATGCGCCCCCAGCACGG GGCCAGAGGAGGAACATGCCCCTGGAGAGGATGAAAGGCATTTCCAGGAGTCTGGAGTCA GTTCTGTCCCTCGGTCCTCGTCCCACAGGGGGTGGTTCAAGCCCCCCTGAAATTCGACAA GCCAGAGCTGTGCCCCAGGGACCTCCAGGCCTGCCTCCACGCCCACCTTTATCCTCTAGC TCTCCTCAGCCCAGCCAGCCCTCTAGGGAGAGGCTTCCCTGGCCCAAAAGAAAACCCCCA CACAATCACCCCATGGGAATGCCTGGAGCCCGTAAAGCCGCTGCCCTCTCTGGAGGCCTC TTGTCCGATCCTGAGTTGCAGAGGAAGATTATGGAGATGGAGCTGAGTGTGCATGGGGTC ACCCACCAGGAGTGCCAGACAGCACTAGGAGCCACTGGGGGAGATGTGGTTTCTGCCATC CGGAACCTCAAGGTAGATCAGCTCTTCCACCTGAGTAGCCGGTCCAGAGCTGACTGCTGG CGCATCCTGGAGCATTACCAGTGGGACCTCTCAGCTGCCAGCCGCTATGTCCTGGCCAGG CCCTGA Clone variation with respect to NM_003985.3 21 c=>t;443 a=>t;1777 g=>a >OriGene 5' read for mutant NM_003985 unedited
ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTTGACCAGGTGGA GCTGGAGACCTGGTCTCTCTAGGGCCTACCCTGAGCTCACCATCTGAAGGAGAGTGCCATCATCCTTAGG AACTCCTTCTCCAGACATGCTTCCTGAGGCTGGCTCTCTGTGGCTACTGAAGCTGCTCCGGGACATCCAG TTGGCCCAGTTTTACTGGCCCATCCTTGAGGAGCTTAATGTCACTCGGCCAGAGCACTTCGACTTTGTAA AGCCTGAGGACCTGGACGGCATTGGCATGGGCCGGCCTGCCCAGCGCAGACTGTCCGAAGCTCTGAAAAG GCTACGTTCTGGGCCTAAGTCTAAGAACTGGGTCTACAAGATCCTTGGAGGTTTTGCCCCTGAGCACAAG AGCCCACCCTGCCCTCGGACAGCCCACGGCACCTCCCTGAGCCAGAGGGGGCCTCAAGTGTCTGATCCCA GAGGGTGCTGTTTGCAGAGGGAGCTGCTGGGTTCAGCTGCTTCGTGTGTGCACCGAGGGCTGTGACGCTG CCCAGTGCAGAGTGTCCAGTGGCTGTCATGTCCCTCCGGGTAGTCCCGAGCCCGATGGCAACAGACTGGG GGACTTCTGCGGAAGGTTTCGTCATGATGAACTTGGAGCCACCCCACACTGCTGCGTCTGCACGCCTTTT ACTGGCCAGCCTTTGCCAATGGTGATGGACTGGCGCCATGGCTCCCTGCCGCGCGCTACGCCCGCCGGAA CCCCCGGTGTCTTGCCTGCTTGCTTTTCTGCGACTGGGGAACCATGGCTACCTGGACAAGCCTGTACGAC CTTGTACCGCACCATGTGTGGTTGCCGGCGCACACATAAGGTGGCGTAATCTTCG >SC323530 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
GMGKCTGGATMTGCTGCTTCGGTGTGGTGCACCGAGGGCTGTGGACGCTGCCCAGTGGCAAGAGTGTCCC AGTGGCTGTCATGTCCCTCCGGGTAGGTCCCGAAGGCCCGATGGGCACAGAACTGGGGGACTTCCTGCGA GAGGTATCGGTCATGATGAACTTGGAGCACCCACACGTGCTGCGTCTGCACGGCCTTGTACTGGGCCAGC CTCTGCAGATGGTGATGGAGCTGGCGCCACTGGGCTCCCTGCACG |
| Restriction Sites | Please inquire |
| ACCN | NM_003985 |
| Insert Size | 2850 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003985.1, NP_003976.1 |
| RefSeq Size | 2771 bp |
| RefSeq ORF | 2001 bp |
| Locus ID | 8711 |
| UniProt ID | Q13470 |
| Domains | pkinase, TyrKc, SH3, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | The protein encoded by this gene belongs to the tyrosine protein kinase family. Tyrosine protein kinases are important regulators of intracellular signal transduction pathways, mediating cellular proliferation, survival, and development. This gene is highly expressed in fetal tissues and at lower levels in few adult tissues, thus may function in signaling pathways utilized broadly during fetal development, and more selectively in adult tissues. It plays a negative regulatory role in the Ras-Raf1-MAPK pathway, and knockout mice have been shown to develop spontaneous tumors, suggesting a role as a tumor suppressor gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] Transcript Variant: This variant (2) uses an alternate in-frame acceptor splice site at an internal coding exon compared to variant 1. This results in a shorter isoform (2) missing a 5 aa protein segment compared to isoform 1. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223073 | TNK1 (Myc-DDK-tagged)-Human tyrosine kinase, non-receptor, 1 (TNK1) |
CNY 4,920.00 |
|
| RC223073L1 | Lenti ORF clone of Human tyrosine kinase, non-receptor, 1 (TNK1), Myc-DDK-tagged |
CNY 7,320.00 |
|
| RC223073L2 | Lenti ORF clone of Human tyrosine kinase, non-receptor, 1 (TNK1), mGFP tagged |
CNY 5,890.00 |
|
| RC223073L3 | Lenti ORF clone of Human tyrosine kinase, non-receptor, 1 (TNK1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC223073L4 | Lenti ORF clone of Human tyrosine kinase, non-receptor, 1 (TNK1), mGFP tagged |
CNY 7,320.00 |
|
| RG223073 | TNK1 (tGFP-tagged) - Human tyrosine kinase, non-receptor, 1 (TNK1) |
CNY 6,520.00 |
|
| SC117643 | TNK1 (untagged)-Human tyrosine kinase, non-receptor, 1 (TNK1) |
CNY 4,888.00 |
