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LATS2 (NM_014572) Human Untagged Clone
CAT＃: SC323550
LATS2 (untagged)-Kinase deficient mutant (K697M) of Human LATS, large tumor suppressor, homolog 2 (Drosophila) (LATS2)
CNY 11,376.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | KPM |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323550 sequence for NM_014572 edited (data generated by NextGen Sequencing)
ATGAGGCCAAAGACTTTTCCTGCCACGACTTATTCTGGAAATAGCCGGCAGCGACTGCAA GAGATTCGTGAGGGGTTAAAACAGCCATCCAAGTCTTCGGTTCAGGGGCTACCCGCAGGA CCAAACAGTGACACTTCCCTGGATGCCAAAGTCCTGGGGAGCAAAGATGCCACCAGGCAG CAGCAGCAGATGAGAGCCACCCCAAAGTTCGGACCTTATCAGAAAGCCTTGAGGGAAATC AGATATTCCTTGTTGCCTTTTGCTAATGAATCGGGCACCTCTGCAGCTGCAGAAGTGAAC CGGCAAATGCTGCAGGAACTGGTGAACGCAGGATGCGACCAGGAGATGGCTGGCCGAGCT CTCAAGCAGACTGGCAGCAGGAGCATCGAGGCCGCCCTGGAGTACATCAGCAAGATGGGC TACCTGGACCCGAGGAATGAGCAGATTGTGCGGGTCATTAAGCAGACCTCCCCAGGAAAG GGGCTCATGCCAACCCCAGTGACGCGGAGGCCCAGCTTCGAAGGAACCGGCGATTCGTTT GCGTCCTACCACCAGCTGAGCGGTACCCCCTACGAGGGCCCAAGCTTCGGCGCTGACGGC CCCACGGCGCTGGAGGAGATGCCGCGGCCGTACGTGGACTACCTTTTCCCCGGAGTCGGC CCCCACGGGCCCGGCCACCAGCACCAGCACCCACCCAAGGGCTACGGTGCCAGCGTAGAG GCAGCAGGGGCACACTTCCCGCTGCAGGGCGCGCACTACGGGCGGCCGCACCTGCTGGTG CCTGGGGAACCCCTGGGCTACGGAGTGCAGCGCAGCCCCTCCTTCCAGAGCAAGACGCCG CCGGAGACCGGGGGTTACGCCAGCCTGCCCACGAAGGGCCAGGGAGGACCGCCAGGCGCC GGCCTCGCTTTCCCACCCCCTGCCGCCGGGCTCTACGTGCCGCACCCACACCACAAGCAG GCCGGTCCCGCGGCCCACCAGCTGCATGTGCTGGGCTCCCGCAGCCAGGTGTTCGCCAGC GACAGCCCCCCGCAGAGCCTGCTCACTCCCTCGCGGAACAGCCTCAACGTGGACCTGTAT GAATTGGGCAGCACCTCCGTCCAGCAGTGGCCGGCTGCCACCCTGGCCCGCCGGGACTCC CTGCAGAAGCCGGGCCTGGAGGCGCCGCCGCGCGCGCACGTGGCCTTCCGGCCTGACTGC CCAGTGCCCAGCAGGACCAACTCCTTCAACAGCCACCAGCCGCGGCCCGGTCCGCCTGGC AAGGCCGAGCCCTCCCTGCCCGCCCCCAACACCGTGACGGCTGTCACGGCCGCGCACATC TTGCACCCGGTGAAGAGCGTGCGTGTGCTGAGGCCGGAGCCGCAGACGGCTGTGGGGCCC TCGCACCCCGCCTGGGTGCCCGCGCCTGCCCCGGCCCCCGCCCCCGCCCCCGCCCCGGCT GCGGAGGGCTTGGACGCCAAGGAGGAGCATGCCCTGGCGCTGGGCGGCGCAGGCGCCTTC CCGCTGGACGTGGAGTACGGAGGCCCAGACCGGAGGTGCCCGCCTCCGCCCTACCCGAAG CACCTGCTGCTGCGCAGCAAGTCGGAGCAGTACGACCTGGACAGCCTGTGCGCAGGCATG GAGCAGAGCCTCCGTGCGGGCCCCAACGAGCCCGAGGGCGGCGACAAGAGCCGCAAAAGC GCCAAGGGGGACAAAGGCGGAAAGGATAAAAAGCAGATTCAGACCTCTCCCGTTCCCGTC CGCAAAAACAGCAGAGACGAAGAGAAGAGAGAGTCACGCATCAAGAGCTACTCGCCATAC GCCTTTAAGTTCTTCATGGAGCAGCACGTGGAGAATGTCATCAAAACCTACCAGCAGAAG GTTAACCGGAGGCTGCAGCTGGAGCAAGAAATGGCCAAAGCTGGACTCTGTGAAGCTGAG CAGGAGCAGATGCGGAAGATCCTCTACCAGAAAGAGTCTAATTACAACAGGTTAAAGAGG GCCAAGATGGACAAGTCTATGTTTGTCAAGATCAAAACCCTGGGGATCGGTGCCTTTGGA GAAGTGTGCCTTGCTTGTAAGGTGGACACTCACGCCCTGTACGCCATGAAGACCCTAAGG AAAAAGGATGTCCTGAACCGGAATCAGGTGGCCCACGTCAAGGCCGAGAGGGACATCCTG GCCGAGGCAGACAATGAGTGGGTGGTCAAACTCTACTACTCCTTCCAAGACAAAGACAGC CTGTACTTTGTGATGGACTACATCCCTGGTGGGGACATGATGAGCCTGCTGATCCGGATG GAGGTCTTCCCTGAGCACCTGGCCCGGTTCTACATCGCAGAGCTGACTTTGGCCATTGAG AGTGTCCACAAGATGGGCTTCATCCACCGAGACATCAAGCCTGATAACATTTTGATAGAT CTGGATGGTCACATTAAACTCACAGATTTCGGCCTCTGCACTGGGTTCAGGTGGACTCAC AATTCCAAATATTACCAGAAAGGGAGCCATGTCAGACAGGACAGCATGGAGCCCAGCGAC CTCTGGGATGATGTGTCTAACTGTCGGTGTGGGGACAGGCTGAAGACCCTAGAGCAGAGG GCGCGGAAGCAGCACCAGAGGTGCCTGGCACATTCACTGGTGGGGACTCCAAACTACATC GCACCCGAGGTGCTCCTCCGCAAAGGGTACACTCAACTCTGTGACTGGTGGAGTGTTGGA GTGATTCTCTTCGAGATGCTGGTGGGGCAGCCGCCCTTTTTGGCACCTACTCCCACAGAA ACCCAGCTGAAGGTGATCAACTGGGAGAACACGCTCCACATTCCAGCCCAGGTGAAGCTG AGCCCTGAGGCCAGGGACCTCATCACCAAGCTGTGCTGCTCCGCAGACCACCGCCTGGGG CGGAATGGGGCCGATGACCTGAAGGCCCACCCCTTCTTCAGCGCCATTGACTTCTCCAGT GACATCCGGAAGCAGCCAGCCCCCTACGTTCCCACCATCAGCCACCCCATGGACACCTCG AATTTCGACCCCGTAGATGAAGAAAGCCCTTGGAACGATGCCAGCGAAGGTAGCACCAAG GCCTGGGACACACTCACCTCGCCCAATAACAAGCATCCTGAGCACGCATTTTACGAATTC ACCTTCCGAAGGTTCTTTGATGACAATGGCTACCCCTTTCGATGCCCAAAGCCTTCAGGA GCAGAAGCTTCACAGGCTGAGAGCTCAGATTTAGAAAGCTCTGATCTGGTGGATCAGACT GAAGGCTGCCAGCCTGTGTACGTGTAG Clone variation with respect to NM_014572.2 >OriGene 5' read for mutant NM_014572 unedited
ACGCCCGCACAGCAAAGGGCGGTAGGCGTTACGGTGGGAGGACATATAAGCAGAGCACATAAAGGCGACA CCATAGAATACAAGCAACTCGAACTCAAAGCAGCGGCCGCGAATACGGCACGAGGAACAAAATCAAAAGG AAATAATAATGAAAGAATGACTACAACAAACTGATACTATTGACAGTATTTTTTTTTTAAATAATAAAAC ACTTTTAAGAAGATTGTATTTATGGTAAAAGGAAACTGGACTAACAATGAGGCCAAAGACTATTCCTGCC ACGACTTATTCTGGAAATAGCCGGCAGCGACTGCAAGAGATTCGTGAGGGGTTAAAGCAGCCATCCAAGA CTTCGGTTCAGGGGCTACCCGCAGGACCAAACAGTGACACTTCCCCTGGATGCCAAAGTCCTGGGGAGCA AAGATGCCACCCAGGCAGCAGCAGCAGATGAGAGCCACCCCCAAAGTTCGGACCTTATCAGAAGCCTTGA GGAAATCAGATATTCTTGTTGCCTTTTGCTAATGATCGGGCACTTCTGCAGCTGCAGAAGTTGAACCGCA ATGCTGCAGACCTGTGACCGCAGAATGCGACCAGAGAATGCCTGCAAGCTTCTCAGGCAAACTGCAGCAG GAGCATCAAGCCGCCTGGAAGTCACTCAGCAGAATGGCCTACCTGAACTAGGAATTGACCAATTGGCCGG TTCATTACCAAACCTCCAGGAAGGCTCTAGTGCCACCATGGACCGAGGCCACTCTCAAGGAACCGGCATT CTTTGGTCTCAACCACTGAACGGTACCCTCAAAGGGCAAGCTTTGGCTGTAAGGCCCAAGGCTTGAGAAT TGCTGCGTCATTGGTAATCTTTCCGAATGGCCGAAGGCGGCAAGACAGAACTCGAGGGTCATGGTGCACG TTAGAGCACCAGGCACATCG >SC323550 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
AAWGGMAGTCTATGTTTGTCAGATCAAAACCCTGGGGATCGGTGCCTTTGGAGAAGTGTGCCTTGCTTGT AAGGTGGACACTCACGCCCTGTACGCCATGATGACCCTAAGGAAAAAGGATGTCCTGAACCGGAATCAGG TGGCCCACGTCAAGGCCGAGAGGGACATCCTGGCCGAGGCAGACAATGAGTGGGTGGTCAAACTCTACTA CTCCTTCCAAGACAAAGACAGCCTGTACTTTGTGATGGACTACAT |
| Restriction Sites | Please inquire |
| ACCN | NM_014572 |
| Insert Size | 4200 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014572.1, NP_055387.1 |
| RefSeq Size | 4098 bp |
| RefSeq ORF | 3267 bp |
| Locus ID | 26524 |
| UniProt ID | Q9NRM7 |
| Domains | UBA, pkinase, S_TK_X, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene encodes a serine/threonine protein kinase belonging to the LATS tumor suppressor family. The protein localizes to centrosomes during interphase, and early and late metaphase. It interacts with the centrosomal proteins aurora-A and ajuba and is required for accumulation of gamma-tubulin and spindle formation at the onset of mitosis. It also interacts with a negative regulator of p53 and may function in a positive feedback loop with p53 that responds to cytoskeleton damage. Additionally, it can function as a co-repressor of androgen-responsive gene expression. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219394 | LATS2 (Myc-DDK-tagged)-Human LATS, large tumor suppressor, homolog 2 (Drosophila) (LATS2) |
CNY 7,576.00 |
|
| RC219394L1 | Lenti ORF clone of Human LATS, large tumor suppressor, homolog 2 (Drosophila) (LATS2), Myc-DDK-tagged |
CNY 9,976.00 |
|
| RC219394L2 | Lenti ORF clone of Human LATS, large tumor suppressor, homolog 2 (Drosophila) (LATS2), mGFP tagged |
CNY 12,730.00 |
|
| RC219394L3 | Lenti ORF clone of Human LATS, large tumor suppressor, homolog 2 (Drosophila) (LATS2), Myc-DDK-tagged |
CNY 9,976.00 |
|
| RC219394L4 | Lenti ORF clone of Human LATS, large tumor suppressor, homolog 2 (Drosophila) (LATS2), mGFP tagged |
CNY 9,976.00 |
|
| RG219394 | LATS2 (tGFP-tagged) - Human LATS, large tumor suppressor, homolog 2 (Drosophila) (LATS2) |
CNY 9,176.00 |
|
| SC120535 | LATS2 (untagged)-Human LATS, large tumor suppressor, homolog 2 (Drosophila) (LATS2) |
CNY 11,376.00 |
