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HIPK3 (NM_005734) Human Untagged Clone
CAT＃: SC323551
HIPK3 (untagged)-Kinase deficient mutant (K226M) of Human homeodomain interacting protein kinase 3 (HIPK3), transcript variant 1
CNY 8,464.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DYRK6; FIST3; PKY; YAK1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_005734, the custom clone sequence may differ by one or more nucleotides
ATGGCCTCACAAGTCTTGGTCTACCCACCATATGTTTATCAAACTCAGTCAAGTGCCTTTTGTAGTGTGA AGAAACTCAAAGTAGAGCCAAGCAGTTGTGTATTCCAGGAAAGAAACTATCCACGGACCTATGTGAATGG TAGAAACTTTGGAAATTCTCATCCTCCCACTAAGGGTAGTGCTTTTCAGACAAAGATACCATTTAATAGA CCTCGAGGACACAACTTTTCATTGCAGACAAGTGCTGTTGTTTTGAAAAACACTGCAGGTGCTACAAAGG TCATAGCAGCTCAGGCACAGCAAGCTCACGTGCAGGCACCTCAGATTGGGGCGTGGCGAAACAGATTGCA TTTCCTAGAAGGCCCCCAGCGATGTGGATTGAAGCGCAAGAGTGAGGAGTTGGATAATCATAGCAGCGCA ATGCAGATTGTCGATGAATTGTCCATACTTCCTGCAATGTTGCAAACCAACATGGGAAATCCAGTGACAG TTGTGACAGCTACCACAGGATCAAAACAGAATTGTACCACTGGAGAAGGTGACTATCAGTTAGTACAGCA TGAAGTCTTATGCTCCATGAAAAATACTTACGAAGTCCTTGATTTTCTTGGTCGAGGCACGTTTGGCCAG GTAGTTAAATGCTGGAAAAGAGGGACAAATGAAATTGTAGCAATCAAAATTTTGAAGAATCATCCTTCTT ATGCCCGTCAAGGTCAAATAGAAGTGAGCATATTAGCAAGGCTCAGTACTGAAAATGCTGATGAATATAA CTTTGTACGAGCTTATGAATGCTTTCAGCACCGTAACCATACTTGTTTAGTCTTTGAGATGCTGGAACAA AACTTGTATGACTTTCTGAAACAAAATAAATTTAGTCCCCTGCCACTAAAAGTGATTCGGCCCATTCTTC AACAAGTGGCCACTGCACTGAAAAAATTGAAAAGTCTTGGTTTAATTCATGCTGATCTCAAGCCAGAGAA TATTATGTTGGTGGATCCTGTTCGGCAGCCTTACAGGGTTAAAGTAATAGACTTTGGGTCGGCCAGTCAT GTATCAAAGACTGTTTGTTCAACATATCTACAATCTCGGTACTACAGAGCTCCAGAGATTATATTGGGGT TGCCATTTTGTGAAGCCATAGACATGTGGTCATTGGGATGTGTGATTGCAGAATTATTTCTTGGATGGCC GCTCTACCCAGGAGCCTTGGAGTATGATCAGATTCGATACATTTCTCAGACTCAAGGTTTGCCAGGAGAA CAGTTGTTAAATGTGGGTACTAAATCCACAAGATTTTTTTGCAAAGAAACAGATATGTCTCATTCTGGTT GGAGATTAAAGACATTGGAAGAGCATGAGGCAGAGACAGGAATGAAGTCTAAAGAAGCCAGAAAATACAT TTTCAACAGTCTGGATGATGTAGCGCATGTGAACACAGTGATGGATTTGGAAGGAAGTGATCTTTTGGCT GAGAAAGCTGATAGAAGAGAATTTGTTAGTCTGTTGAAGAAAATGTTGCTGATTGATGCAGATTTAAGAA TTACTCCAGCTGAGACCCTGAACCATCCTTTTGTTAATATGAAACATCTTCTAGATTTCCCTCATAGCAA CCATGTAAAGTCCTGTTTTCATATTATGGATATTTGTAAGTCCCACCTAAATTCATGTGACACAAATAAT CACAACAAAACTTCACTTTTAAGACCAGTTGCTTCAAGCAGTACTGCTACACTGACTGCAAATTTTACTA AAATCGGAACATTAAGAAGTCAGGCATTGACCACATCTGCTCATTCAGTTGTGCACCATGGAATACCTCT GCAGGCAGGAACTGCTCAGTTTGGTTGTGGTGATGCTTTTCAGCAGACATTGATTATCTGTCCCCCAGCT ATTCAAGGTATTCCTGCAACACATGGTAAACCCACCAGTTATTCAATAAGGGTAGATAATACAGTTCCAC TTGTAACTCAGGCCCCAGCTGTGCAGCCACTACAGATCCGACCAGGAGTTCTTTCTCAGACGTGGTCTGG TAGAACACAGCAGATGCTGGTGCCTGCCTGGCAACAGGTGACACCCCTGGCTCCTGCTACTACTACACTA ACTTCTGAGAGTGTGGCTGGTTCACACAGGCTTGGAGACTGGGGGAAGATGATTTCATGCAGCAATCATT ATAACTCAGTGATGCCGCAGCCTCTTCTGACCAATCAGATAACTTTATCTGCCCCTCAGCCAGTTAGTGT GGGGATTGCACATGTTGTCTGGCCTCAGCCTGCCACTACCAAGAAAAATAAACAGTGCCAGAACAGAGGT ATTTTGGTAAAACTAATGGAATGGGAGCCAGGAAGAGAGGAAATAAATGCTTTCAGTTGGAGTAATTCAT TACAGAATACCAATATCCCACATTCAGCATTTATTTCTCCAAAGATAATTAATGGGAAAGATGTCGAGGA AGTAAGTTGTATAGAAACACAGGACAATCAGAACTCAGAAGGAGAGGCAAGAAATTGCTGTGAAACATCT ATCAGACAGGACTCTGATTCATCAGTTTCAGACAAACAGCGGCAAACCATCATTATTGCCGACTCCCCGA GTCCTGCAGTGAGTGTCATCACTATCAGCAGTGACACTGATGAGGAAGAGACTTCCCAGAGACATTCACT CAGAGAATGTAAAGGTAGTCTAGATTGTGAAGCTTGCCAGAGCACTTTGAATATTGATCGGATGTGTTCA TTAAGTAGTCCTGATAGTACTCTGAGTACCAGCTCCTCAGGGCAGTCCAGCCCATCCCCCTGCAAGAGAC CGAATAGTATGTCAGATGAAGAGCAAGAAAGTAGTTGTGATACGGTGGATGGCTCTCCGACATCTGACTC TTCCGGGCATGACAGTCCATTTGCAGAGAGCACTTTTGTGGAGGACACTCATGAAAACACAGAATTGGTA TCCTCTGCTGACACAGAAACCAAGCCAGCTGTCTGTTCTGTTGTGGTGCCACCAGTGGAACTAGAAAATG GCTTAAATGCCGATGAGCATATGGCAAACACAGATTCTATATGCCAGCCATTAATAAAAGGACGATCTGC CCCTGGAAGATTAAACCAGCCTTCTGCAGTGGGTACTCGTCAGCAAAAATTGACATCAGCATTCCAGCAG CAGCATTTGAACTTCAGTCAGGTTCAGCACTTTGGATCTGGGCATCAAGAGTGGAATGGAAACTTTGGGC ACAGAAGACAGCAAGCTTATATTCCTACTAGTGTTACCAGTAATCCATTCACTCTTTCTCATGGAAGTCC CAATCACACAGCAGTGCATGCCCACCTGGCTGGAAATACACACCTCGGAGGACAGCCTACTCTACTTCCA TACCCATCATCAGCCACCCTCAGTAGTGCTGCACCAGTGGCCCACCTGTTAGCCTCTCCGTGTACCTCAA GACCTATGTTACAGCATCCAACTTATAATATCTCCCATCCCAGTGGCATAGTTCACCAAGTCCCAGTGGG CTTAAATCCCCGTCTGTTACCATCCCCAACCATTCATCAGACTCAGTACAAACCAATCTTCCCACCACAT TCTTACATTGCAGCATCACCTGCATATACTGGATTTCCACTGAGTCCAACAAAACTCAGCCAGTATCCAT ATATGTGA >OriGene 5' read for mutant NM_005734 unedited
ACCCGCCCGTTGCAGCAATGGGCGGTAGGCGTGTACAGGAGGGAGGTCTATATAAGCAGAGCTCGTTTAG TGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAAGATGAGG GAGACGGGCCCGGCGCTTAGCAGCCAGAGCAGCAGCAGCAGCAGCAGCGGTCGGGGGAGGGTGTTTCGCC GTTTCCTCTCAGCCGCCAGGACAAGATGGCAGCGGCCGCGGAGAGGGGCTGAGCCCGGGCTGGGTGGTGC CGCCTGCTGAAGCGCCTGGCTCCCGGTCCCCGGCACGGCCCTGCGCCCCCCCCCCGGACATGCTCAGGGC TGCGGGCCGCCCGAAAAAGGAGAGAGCGCGGGCCTCTAGGAAGGTATGGCCTCCCAAGTCTGGGTCTAAC CCCCCATATGTTTATTCAAATCAGTCCAATGGCCTTTGGTATGGGGAGAAAACTCAAAGTAAAAGCCAAC CAGTTGGGTTTTCCAGGAAAAAAACTATCCCCCGGACCTTTGGGATGGGGAAAAACTTTGGAAAATTCCC ATCTTCCCCCAAGGGGTAGGCCTTTTCAAAACAAAAATCCCTTTTAATAGACCCTCGGGGACCAACTTTT TCATTTCCAAAAAAAGGCTTTTGTTTTGAAAAAACCCTGGAGGGGCCCCCAAAGGGTAAAAGCCTCTAGG GCACACAGTTCCCTGTCGCGGGCCCCTAAAAGGGGGGGTGTGGAAAAAAAAATGTGTTTTTCATAAGAGC CCCCCCCACATGTGTGTTTTGCGCCCCAGAGGAGTGAGGTGTGTGATAATCTATACGCCCAGTCGCGATG TGTGTCAAGATATTCGCACTCTCCTCCGCGTGTGTGACACACAGTGAGAATCCGCTGCGTGTTGACACAC CACAGCTCACAATTATTCCCCGGAGGAGAGGTATCTATATTATTCACGCGGAGTATTTCTCGCCGCAAAA TATACATGACTAG >SC323551 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
ARTCTTGWTTTCTTGGTCGAGGCACGTTTGGCCAGGTAGTTAAATGCTGGAAAAGAGGGACAAATGAAAT TGTAGCAATCATGATTTTGAAGAATCATCCTTCTTATGCCCGTCAAGGTCAAATAGAAGTGAGCATATTA GCAAGGCTCAGTACTGAAAATGCTGATGAATATAACTTTGTACGAGCTTATGAATGCTTTCAGCACCGTA ACCATACTTGTTTAGTCTTTGAGATGCTGGAACAAAACTTGTATG |
| Restriction Sites | Please inquire |
| ACCN | NM_005734 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005734.2, NP_005725.2 |
| RefSeq Size | 3657 bp |
| RefSeq ORF | 3648 bp |
| Locus ID | 10114 |
| UniProt ID | Q9H422 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase, Transcription Factors |
| Gene Summary | Serine/threonine-protein kinase involved in transcription regulation, apoptosis and steroidogenic gene expression. Phosphorylates JUN and RUNX2. Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors. The phosphorylation of NR5A1 activates SF1 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. In osteoblasts, supports transcription activation: phosphorylates RUNX2 that synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Increased expression of miR-187 in human islets from individuals with type 2 diabetes is associated with reduced glucose-stimulated insulin secretion
,Locke, JM;da Silva Xavier, G;Dawe, HR;Rutter, GA;Harries, LW;,
Diabetologia. 2013 Oct 23
,PubMed ID 24149837
[HIPK3]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214196 | HIPK3 (Myc-DDK-tagged)-Human homeodomain interacting protein kinase 3 (HIPK3), transcript variant 1 |
CNY 8,464.00 |
|
| RC214196L1 | Lenti ORF clone of Human homeodomain interacting protein kinase 3 (HIPK3), transcript variant 1, Myc-DDK-tagged |
CNY 10,864.00 |
|
| RC214196L2 | Lenti ORF clone of Human homeodomain interacting protein kinase 3 (HIPK3), transcript variant 1, mGFP tagged |
CNY 14,060.00 |
|
| RC214196L3 | Lenti ORF clone of Human homeodomain interacting protein kinase 3 (HIPK3), transcript variant 1, Myc-DDK-tagged |
CNY 14,060.00 |
|
| RC214196L4 | Lenti ORF clone of Human homeodomain interacting protein kinase 3 (HIPK3), transcript variant 1, mGFP tagged |
CNY 14,060.00 |
|
| RG214196 | HIPK3 (tGFP-tagged) - Human homeodomain interacting protein kinase 3 (HIPK3), transcript variant 1 |
CNY 13,400.00 |
|
| SC315273 | HIPK3 (untagged)-Human homeodomain interacting protein kinase 3 (HIPK3), transcript variant 1 |
CNY 23,470.00 |
