MAK (NM_005906) Human Untagged Clone
CAT#: SC323564
MAK (untagged)-Kinase deficient mutant (K33M) of Human male germ cell-associated kinase (MAK)
CNY 4,648.00
Product images
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Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | RP62 |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC323564 sequence for NM_005906 edited (data generated by NextGen Sequencing)
ATGAACCGATACACAACCATGAGACAGTTGGGGGACGGCACGTATGGGAGTGTGCTTATG GGCAAGAGTAATGAATCCGGGGAGCTGGTGGCCATCAAAAGGATGAAGAGAAAGTTCTAT TCTTGGGATGAATGCATGAACTTGAGAGAAGTTAAGTCTCTGAAGAAACTTAATCATGCC AATGTTATTAAATTGAAAGAAGTTATCAGAGAAAATGACCATCTTTATTTTATATTTGAA TATATGAAAGAAAACCTCTATCAATTAATGAAAGACAGAAACAAGTTGTTCCCTGAATCA GTCATCAGAAATATTATGTATCAAATATTGCAAGGGCTGGCTTTTATCCATAAACATGGC TTTTTTCATAGGGACATGAAACCAGAAAACTTGCTTTGTATGGGTCCAGAGCTTGTGAAA ATTGCTGATTTTGGACTTGCAAGAGAATTAAGGTCACAGCCACCATACACTGATTATGTA TCTACCAGATGGTATCGTGCCCCTGAAGTTTTACTGAGATCTTCAGTTTATAGTTCTCCC ATTGATGTGTGGGCTGTTGGAAGTATCATGGCTGAACTCTATATGTTAAGGCCACTTTTC CCAGGGACAAGTGAGGTCGATGAAATCTTTAAAATTTGCCAAGTTTTAGGGACTCCCAAA AAAAGTGACTGGCCAGAAGGATACCAGCTGGCATCCTCTATGAACTTCCGTTTTCCCCAG TGTGTTCCTATAAACTTAAAAACTCTTATTCCCAATGCCAGTAATGAAGCTATTCAGCTC ATGACCGAAATGTTGAATTGGGATCCAAAGAAACGACCGACAGCAAGCCAGGCATTGAAA CACCCATATTTTCAAGTTGGTCAGGTATTAGGCCCTTCGTCAAATCATCTGGAATCAAAA CAGTCTTTAAATAAGCAGCTGCAACCATTAGAATCAAAGCCATCTTTAGTTGAGGTAGAG CCTAAGCCTCTGCCGGATATAATCGATCAGGTTGTTGGACAACCCCAGCCAAAAACTAGC CAGCAGCCACTGCAGCCCATTCAGCCGCCACAGAACCTGAGCGTCCAGCAACCTCCAAAG CAACAGAGTCAGGAGAAACCGCCACAAACGCTATTCCCGAGCATCGTCAAAAACATGCCA ACTAAGCCAAATGGCACACTGAGTCATAAAAGTGGTAGGAGGCGTTGGGGTCAGACTATC TTCAAGTCTGGAGATAGCTGGGAAGAGTTGGAGGACTATGATTTCGGAGCCTCCCATTCC AAGAAGCCAAGCATGGGTGTTTTTAAAGAAAAAAGGAAAAAAGATTCTCCATTTCGGCTT CCAGAGCCAGTACCCTCAGGCTCCAACCACTCGACAGGGGAAAACAAGAGCTTACCTGCT GTTACTTCCCTAAAATCTGATTCCGAATTGTCAACTGCTCCAACCTCTAAACAGTACTAC TTGAAACAATCAAGATATCTTCCAGGTGTGAATCCCAAGAAGGTGTCCTTGATAGCCAGT GGAAAGGAAATAAACCCCCACACTTGGAGCAACCAGTTATTCCCCAAGTCACTGGGACCC GTTGGGGCAGAACTTGCTTTCAAAAGGAGCAATGCAGGAAATCTTGGAAGTTATGCTACT TACAATCAGTCAGGATATATTCCTTCCTTTCTCAAAAAAGAAGTGCAGTCAGCTGGCCAG AGGATCCACTTAGCACCTCTCAATGCAACGGCTTCAGAATATACCTGGAACACAAAAACT GGTCGGGGGCAGTTTTCAGGACGTACTTATAATCCTACAGCAAAAAACCTAAATATTGTG AACCGTGCACAGCCCATTCCCTCAGTGCACGGGAGGACAGACTGGGTGGCCAAGTATGGA GGCCACCGGTAG Clone variation with respect to NM_005906.4 1830 t=>c >OriGene 5' read for mutant NM_005906 unedited
ACCGCTCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTAATCCCCAGCGG GCTGCAGGGCCACCACCCTCTGTCCGACCCAGCCGGAGCCCGACCTGCGAGGCTGGCCGGGCATGTCCCC TCGGCGCCCTGTGCTACCAGGGCCAATCTCCGCCGGGAGACCGGTCCCTGACGCCGGGTGTTTATAAGAA TGGAAGTGTTGTTTCCTTGCCCGATTCCTTCATGCTATATCTCATGAACCTCTGTAATCTTGGGGGAGAG ACTATATTTAATGATGACAAACCTGTCACCAGTGTAGCACCAACAGTGGGGAGGCCAAAACCAAAAAAAA TTAAGAACCGTTCAATTTATATTCAACAAGGAAGTCTTTTCATCACCAACTTCGGCTGCATTATTTTTCC GAATAACCCGACCCCAACCAGGAAACAGTTGGGGGGACGGCCCGTTGGGAAGTGCCTTAGGGCCAAGAGT AAGGATACCGGGGACGGGGGGCACCGGGGGTAGAAAAAAATTTCATTTCTTGGGATGAGTGTGAGACCTG AGAAATTTATCCTCAAAAAACTACTGCCCAGTGTTATATTTTGAAATTTTCAAAAAGAGCACTCTTTTTA TTTTAATTTTGAAAAAACCCTTCATTTTTAAGAGAAAACCTTTGCCCGACATCTCCAAATATTTTAAATG CGGGCGCGGCTATCACAAGCGTTTTAGAGAGACCCAATCTGTTGGTGACAAGTTATACTCGTGTCGTGCG AAA >SC323564 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CCATGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTAATCCCCAGCGGGCTGCA GGGCCACCACCCTCTGTCCGACCCAGCCGGAGCCCGACCTGCGAGGCTGGCCGGGCATGTCCCCTCGGCG CCCTGTGCTACCAGGGCCAATCTCCGCCGGGAGACCGGTCCCTGA |
Restriction Sites | Please inquire |
ACCN | NM_005906 |
Insert Size | 3200 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005906.3, NP_005897.1 |
RefSeq Size | 3830 bp |
RefSeq ORF | 1872 bp |
Locus ID | 4117 |
UniProt ID | P20794 |
Domains | pkinase, TyrKc, S_TKc |
Protein Families | Druggable Genome, Protein Kinase |
Gene Summary | The product of this gene is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired. Mutations in this gene have been associated with ciliary defects resulting in retinitis pigmentosa 62. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016] Transcript Variant: This variant (1) encodes isoform 1. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC213532 | MAK (Myc-DDK-tagged)-Human male germ cell-associated kinase (MAK) |
CNY 4,640.00 |
|
RC213532L1 | Lenti ORF clone of Human male germ cell-associated kinase (MAK), Myc-DDK-tagged |
CNY 7,040.00 |
|
RC213532L2 | Lenti ORF clone of Human male germ cell-associated kinase (MAK), mGFP tagged |
CNY 5,890.00 |
|
RC213532L3 | Lenti ORF clone of Human male germ cell-associated kinase (MAK), Myc-DDK-tagged |
CNY 7,040.00 |
|
RC213532L4 | Lenti ORF clone of Human male germ cell-associated kinase (MAK), mGFP tagged |
CNY 7,040.00 |
|
RG213532 | MAK (tGFP-tagged) - Human male germ cell-associated kinase (MAK) |
CNY 6,240.00 |
|
SC116439 | MAK (untagged)-Human male germ cell-associated kinase (MAK) |
CNY 4,648.00 |