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CDC42 binding protein kinase alpha (CDC42BPA) (NM_014826) Human Untagged Clone
CAT＃: SC323610
CDC42BPA (untagged)-Kinase deficient mutant (K106M) of Human CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant A
CNY 11,216.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MRCK; MRCKA; PK428 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323610 sequence for NM_014826 edited (data generated by NextGen Sequencing)
ATGTCTGGAGAAGTGCGTTTGAGGCAGTTGGAGCAGTTTATTTTGGACGGGCCCGCTCAG ACCAATGGGCAGTGCTTCAGTGTGGAGACGTTACTGGATATACTCATCTGCCTTTATGAT GAATGCAATAATTCTCCATTGAGAAGAGAGAAGAACATTCTCGAATACCTAGAATGGGCT AAACCATTTACTTCTAAAGTGAAACAAATGCGATTACATAGAGAAGACTTTGAAATATTA AAGGTGATTGGTCGAGGAGCTTTTGGGGAGGTTGCTGTAGTAAAACTAAAAAATGCAGAT AAAGTGTTTGCCATGAAAATATTGAATAAATGGGAAATGCTGAAAAGAGCTGAGACAGCA TGTTTTCGTGAAGAAAGGGATGTATTAGTGAATGGAGACAATAAATGGATTACAACCTTG CACTATGCTTTCCAGGATGACAATAACTTATACCTGGTTATGGATTATTATGTTGGTGGG GATTTGCTTACTCTACTCAGCAAATTTGAAGATAGATTGCCTGAAGATATGGCTAGATTT TACTTGGCTGAGATGGTGATAGCAATTGACTCAGTTCATCAGCTACATTATGTACACAGA GACATTAAACCTGACAATATACTGATGGATATGAATGGACATATTCGGTTAGCAGATTTT GGTTCTTGTCTGAAGCTGATGGAAGATGGAACGGTTCAGTCCTCAGTGGCTGTAGGAACT CCAGATTATATCTCTCCTGAAATCCTTCAAGCCATGGAAGATGGAAAAGGGAGATATGGA CCTGAATGTGACTGGTGGTCTTTGGGGGTCTGTATGTATGAAATGCTTTACGGAGAAACA CCATTTTATGCAGAATCGCTGGTGGAGACATACGGAAAAATCATGAACCACAAAGAGAGG TTTCAGTTTCCAGCCCAAGTGACTGATGTGTCTGAAAATGCTAAGGATCTTATTCGAAGG CTCATTTGTAGCAGAGAACATCGACTTGGTCAAAATGGAATAGAAGACTTTAAGAAACAC CCATTTTTCAGTGGAATTGATTGGGATAATATTCGGAACTGTGAAGCACCTTATATTCCA GAAGTTAGTAGCCCAACAGATACATCGAATTTTGATGTAGATGATGATTGTTTAAAAAAT TCTGAAACGATGCCCCCACCAACACATACTGCATTTTCTGGCCACCATCTGCCATTTGTT GGTTTTACATATACTAGTAGCTGTGTACTTTCTGATCGGAGCTGTTTAAGAGTTACGGCT GGTCCCACCTCACTGGATCTTGATGTTAATGTTCAGAGGACTCTAGACAACAACTTAGCA ACTGAAGCTTATGAAAGAAGAATTAAGCGCCTTGAGCAAGAAAAACTTGAACTCAGTAGA AAACTTCAAGAGTCAACACAGACTGTCCAAGCTCTGCAGTATTCAACTGTTGATGGTCCA CTAACAGCAAGCAAAGATTTAGAAATAAAAAACTTAAAAGAAGAAATTGAAAAACTAAGA AAACAAGTAACAGAATCAAGTCATTTGGAACAGCAACTTGAAGAAGCTAATGCTGTGAGG CAAGAACTAGATGATGCTTTTAGACAAATCAAGGCTTATGAAAAACAAATCAAAACGTTA CAACAAGAAAGAGAAGATCTAAATAAGCTGGAAGTTCATACAGAAGCTCTAGCTGCTGAA GCATCTAAAGACAGGAAGCTACGTGAACAGAGTGAGCACTATTCTAAGCAACTGGAAAAT GAATTGGAGGGACTGAAGCAAAAACAAATTAGTTACTCACCAGGAGTATGCAGCATAGAA CATCAGCAAGAGATAACCAAACTAAAGACTGATTTGGAAAAGAAAAGTATCTTTTATGAA GAAGAATTATCTAAAAGAGAAGGAATACATGCAAATGAAATAAAAAATCTTAAGAAAGAA CTGCATGATTCAGAAGGTCAGCAACTTGCTCTCAACAAAGAAATTATGATTTTAAAAGAC AAATTGGAAAAAACCAGAAGAGAAAGTCAAAGTGAAAGGGAGGAATTTGAAAGTGAGTTC AAACAACAATATGAACGAGAAAAAGTGTTGTTAACTGAAGAAAATAAAAAGCTGACGAGT GAACTTGATAAGCTTACTACTTTGTATGAGAACTTAAGTATACACAACCAGCAGTTAGAA GAAGAGGTTAAAGATCTAGCAGACAAGAAAGAATCAGTTGCACATTGGGAAGCCCAAATC ACAGAAATAATTCAGTGGGTCAGCGATGAAAAGGATGCACGAGGGTATCTTCAGGCCTTA GCTTCTAAAATGACTGAAGAATTGGAGGCATTAAGAAATTCCAGCTTGGGTACACGAGCA ACAGATATGCCCTGGAAAATGCGTCGTTTTGCGAAACTGGATATGTCAGCTAGACTGGAG TTGCAGTCGGCTCTGGATGCAGAAATAAGAGCCAAACAGGCCATCCAAGAAGAGTTGAAT AAAGTTAAAGCATCTAATATCATAACAGAATGTAAACTAAAAGATTCAGAGAAGAAGAAC TTGGAACTACTCTCAGAAATCGAACAGCTGATAAAGGACACTGAAGAGCTTAGATCTGAA AAGGGTATAGAGCACCAAGACTCACAGCATTCTTTCTTGGCATTTTTGAATACGCCTACC GATGCTCTGGATCAATTTGAAACTGTAGACTCCACTCCACTTTCAGTTCACACACCAACC TTAAGGAAAAAAGGATGTCCTGGTTCAACTGGCTTTCCACCTAAGCGCAAGACTCACCAG TTTTTTGTAAAATCTTTTACTACTCCTACCAAGTGTCATCAGTGTACCTCCTTGATGGTG GGTTTAATAAGACAGGGCTGTTCATGTGAAGTGTGTGGATTCTCATGCCATATAACTTGT GTAAACAAAGCTCCAACCACTTGTCCAGTTCCTCCTGAACAGACAAAAGGTCCCCTGGGT ATAGATCCTCAGAAAGGAATAGGAACAGCATATGAAGGTCATGTCAGGATTCCTAAGCCA GCTGGAGTGAAGAAAGGGTGGCAGAGAGCACTGGCTATAGTGTGTGACTTCAAACTCTTT CTGTACGATATTGCTGAAGGAAAAGCATCTCAGCCCAGTGTTGTCATTAGTCAAGTGATT GACATGAGGGATGAAGAATTTTCTGTGAGTTCAGTCTTGGCTTCTGATGTTATCCATGCA AGTCGGAAAGATATACCCTGTATATTTAGGGTCACAGCTTCCCAGCTCTCAGCATCTAAT AACAAATGTTCAATCCTGATGCTAGCAGACACTGAGAATGAGAAGAATAAGTGGGTGGGA GTGCTGAGTGAATTGCACAAGATTTTGAAGAAAAACAAATTCAGAGACCGCTCAGTCTAT GTTCCCAAAGAGGCTTATGACAGCACTCTACCCCTCATTAAAACAACCCAGGCAGCCGCA ATCATAGATCATGAAAGAATTGCTTTGGGAAACGAAGAAGGGTTATTTGTTGTACATGTC ACCAAAGATGAAATTATTAGAGTTGGTGACAATAAGAAGATTCATCAGATTGAACTCATT CCAAATGATCAGCTTGTTGCTGTGATCTCAGGACGAAATCGTCATGTACGACTTTTTCCT ATGTCAGCATTGGATGGGCGAGAGACCGATTTTTACAAGCTGTCAGAAACTAAAGGGTGT CAAACCATAACTTCTGGAAAGGTGCGCCATGGAGCTCTCACATGCCTGTGTGTGGCTATG AAAAGGCAGGTCCTCTGTTATGAACTATTTCAGAGCAAGACCCGTCACAGAAAATTTAAG GAAATTCAAGTCCCATATAATGTCCAGTGGATGGCAATCTTCAGTGAACAACTCTGTGTG GGATTCCAGTCAGGATTTCTAAGATACCCCTTGAATGGAGAAGGAAATCCATACAGTATG CTCCATTCAAATGACCATACACTATCATTTATTGCACATCAACCAATGGATGCTATCTGC GCAGTTGAGATCTCCAGTAAAGAATATCTGCTGTGTTTTAACAGCATTGGGATATACACT GACTGCCAGGGCCGAAGATCTAGACAACAGGAATTGATGTGGCCAGCAAATCCTTCCTCT TGTTGTTACAATGCACCATATCTCTCGGTGTACAGTGAAAATGCAGTTGATATCTTTGAT GTGAACTCCATGGAATGGATTCAGACTCTTCCTCTCAAAAAGGTTCGACCCTTAAACAAT GAAGGATCATTAAATCTTTTAGGGTTGGAGACCATTAGATTAATATATTTCAAAAATAAG ATGGCAGAAGGGGACGAACTGGTAGTACCTGAAACATCAGATAATAGTCGGAAACAAATG GTTAGAAACATTAACAATAAGCGGCGTTATTCCTTCAGAGTCCCAGAAGAGGAAAGGATG CAGCAGAGGAGGGAAATGCTACGAGATCCAGAAATGAGAAATAAATTAATTTCTAATCCA ACTAATTTTAATCACATAGCACACATGGGTCCTGGAGATGGAATACAGATCCTGAAAGAT CTGCCCATGAACCCTCGGCCTCAGGAAAGTCGGACAGTATTCAGTGGCTCAGTCAGTATT CCATCTATCACCAAATCCCGCCCTGAGCCAGGCCGCTCCATGAGTGCTAGCAGTGGCTTG TCAGCAAGGTCATCCGCACAGAATGGCAGCGCATTAAAGAGGGAATTCTCTGGAGGAAGC TACAGTGCCAAGCGGCAGCCCATGCCCTCCCCGTCAGAGGGCTCTTTGTCCTCCGGAGGC ATGGACCAAGGAAGTGATGCCCCAGCGAGGGACTTTGACGGAGAGGACTCTGACTCTCCG AGGCATTCCACAGCTTCCAACAGTTCCAACCTAAGCAGCCCCCCAAGCCCAGTTTCACCC CGAAAAACCAAGAGCCTCTCCCTGGAGAGCACTGACCGCGGGAGCTGGGACCCGTGA Clone variation with respect to NM_014826.4 90 a=>g;3667 g=>a;3780 a=>g;4734 t=>c;4853 c=>t >OriGene 5' read for mutant NM_014826 unedited
TCCGTCCCGTCTGAGCAACGGGCGGTTGGCGCTGTACTGGCTGGGAGGTCTATATAAGCAGAGCTCGTTT AGTGAACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAAAATGA AGAGTTTTCACCGGAATCCGTTGAAAATAGGACCGACCGCAAAGCCTTAAAGAAAGAAGGACCTCGGGAG GAGAAACGAAAAGCCGCCTCCGGGCAAGACTTGGCGTGCTCCGAGCCGAGGGGCTGCTTTCAGGGACCTC GCCCCCCTCCCTTTCCTGCTGGAGAAAATTGCCGCTGATGCATTATCCAAGTGGTGGGTTGGGGAGGGAT TTTGCAGCAACATTTTTTGTTTTTCCCCTCCCCCCTTCTATGCATTTCTGTTTTTTTTCCCTCCCCTTTC TTGTTTTTCTTTCTCCCGGGAAAGTGAATTGCTGATGCAATCGGGACTTTTATTTCATTATGGATGCACC CGGATTGGATCAGGATACGTGGAACAATTTGAATTTGGATTGAAGGAGAAAAGTTTTTTTTTTTAAAAAA GTGGTGACCCCAAGTCCGTTGAACTTTAATATATATTTAATTAAAATATCACACTTATTGATTTCTTTAA AATGCTATAGATATATTTTTATGGTATTTACCCTCAAATAATGTATTGGGTTAAATGAAAACGCTCAGTT AGTGAGGTACTGGTGTGTTGATGTTAATTCACACCGCATGCTGACGTGTTGATACCAAGGTATTTAAACA CCTGACTAGTCCAATGTAAGTCCTATATAGAACCGAATCGGCACGCTCGGAATGCTTGACTGACATATGA AGCGCGTACTGACGCTATGGACTTCGATACCAGCTAA >SC323610 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TKCATTTAAGGTGATTGGTCGAGGAGCTTTTGGGGAGGTTGCTGTAGTAAAACTAAAAAATGCMGATAAA GTGTTTGCCATGATGATATTGAATAAATGGGAAATGCTGAAAAGAGCTGAGACAGCATGTTTTCGTGAAG AAAGGGATGTATTAGTGAATGGAGACAATAAATGGATTACAACCTTGCACTATGCTTTCCAGGATGACAA TAACTTATACCTGGTTATGGATTATTATGTTGGTGGGGATTTGCT |
| Restriction Sites | Please inquire |
| ACCN | NM_014826 |
| Insert Size | 6200 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014826.3, NP_055641.3 |
| RefSeq Size | 7776 bp |
| RefSeq ORF | 4917 bp |
| Locus ID | 8476 |
| UniProt ID | Q5VT25 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase contains multiple functional domains. Its kinase domain is highly similar to that of the myotonic dystrophy protein kinase (DMPK). This kinase also contains a Rac interactive binding (CRIB) domain, and has been shown to bind CDC42. It may function as a CDC42 downstream effector mediating CDC42 induced peripheral actin formation, and promoting cytoskeletal reorganization. Multiple alternatively spliced transcript variants have been described. [provided by RefSeq, Sep 2018] Transcript Variant: This variant (A) lacks a coding segment compared to variant B, resulting in an isoform (A) that lacks an internal region, as compared to isoform B. |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222587 | CDC42BPA (Myc-DDK-tagged)-Human CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant A |
CNY 11,792.00 |
|
| RC222587L3 | Lenti-ORF clone of CDC42BPA (Myc-DDK-tagged)-Human CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant A |
CNY 19,380.00 |
|
| RC222587L4 | Lenti-ORF clone of CDC42BPA (mGFP-tagged)-Human CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant A |
CNY 19,380.00 |
|
| RG222587 | CDC42BPA (tGFP-tagged) - Human CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant A |
CNY 13,392.00 |
|
| SC114791 | CDC42BPA (untagged)-Human CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant A |
CNY 11,216.00 |
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