United States
Germany
Japan
United Kingdom
China
BMPR1A (NM_004329) Human Untagged Clone
CAT＃: SC323635
BMPR1A (untagged)-Kinase deficient mutant (K261M) of Human bone morphogenetic protein receptor, type IA (BMPR1A)
CNY 3,968.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | 10q23del; ACVRLK3; ALK3; CD292; SKR5 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_004329, the custom clone sequence may differ by one or more nucleotides
ATGCCTCAGCTATACATTTACATCAGATTATTGGGAGCCTATTTGTTCATCATTTCTCGTGTTCAAGGAC AGAATCTGGATAGTATGCTTCATGGCACTGGGATGAAATCAGACTCCGACCAGAAAAAGTCAGAAAATGG AGTAACCTTAGCACCAGAGGATACCTTGCCTTTTTTAAAGTGCTATTGCTCAGGGCACTGTCCAGATGAT GCTATTAATAACACATGCATAACTAATGGACATTGCTTTGCCATCATAGAAGAAGATGACCAGGGAGAAA CCACATTAGCTTCAGGGTGTATGAAATATGAAGGATCTGATTTTCAGTGCAAAGATTCTCCAAAAGCCCA GCTACGCCGGACAATAGAATGTTGTCGGACCAATTTATGTAACCAGTATTTGCAACCCACACTGCCCCCT GTTGTCATAGGTCCGTTTTTTGATGGCAGCATTCGATGGCTGGTTTTGCTCATTTCTATGGCTGTCTGCA TAATTGCTATGATCATCTTCTCCAGCTGCTTTTGTTACAAACATTATTGCAAGAGCATCTCAAGCAGACG TCGTTACAATCGTGATTTGGAACAGGATGAAGCATTTATTCCAGTTGGAGAATCACTAAAAGACCTTATT GACCAGTCACAAAGTTCTGGTAGTGGGTCTGGACTACCTTTATTGGTTCAGCGAACTATTGCCAAACAGA TTCAGATGGTCCGGCAAGTTGGTAAAGGCCGATATGGAGAAGTATGGATGGGCAAATGGCGTGGCGAAAA AGTGGCGGTGAAAGTATTCTTTACCACTGAAGAAGCCAGCTGGTTTCGAGAAACAGAAATCTACCAAACT GTGCTAATGCGCCATGAAAACATACTTGGTTTCATAGCGGCAGACATTAAAGGTACAGGTTCCTGGACTC AGCTCTATTTGATTACTGATTACCATGAAAATGGATCTCTCTATGACTTCCTGAAATGTGCTACACTGGA CACCAGAGCCCTGCTTAAATTGGCTTATTCAGCTGCCTGTGGTCTGTGCCACCTGCACACAGAAATTTAT GGCACCCAAGGAAAGCCCGCAATTGCTCATCGAGACCTAAAGAGCAAAAACATCCTCATCAAGAAAAATG GGAGTTGCTGCATTGCTGACCTGGGCCTTGCTGTTAAATTCAACAGTGACACAAATGAAGTTGATGTGCC CTTGAATACCAGGGTGGGCACCAAACGCTACATGGCTCCCGAAGTGCTGGACGAAAGCCTGAACAAAAAC CACTTCCAGCCCTACATCATGGCTGACATCTACAGCTTCGGCCTAATCATTTGGGAGATGGCTCGTCGTT GTATCACAGGAGGGATCGTGGAAGAATACCAATTGCCATATTACAACATGGTACCGAGTGATCCGTCATA CGAAGATATGCGTGAGGTTGTGTGTGTCAAACGTTTGCGGCCAATTGTGTCTAATCGGTGGAACAGTGAT GAATGTCTACGAGCAGTTTTGAAGCTAATGTCAGAATGCTGGGCCCACAATCCAGCCTCCAGACTCACAG CATTGAGAATTAAGAAGACGCTTGCCAAGATGGTTGAATCCCAAGATGTAAAAATCTGA >OriGene 5' read for mutant NM_004329 unedited
TCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGGAAGGCTTAGGCTCG GCGCGTCCGTCCGCGCGCGGCGAAGATCGCACGGCCCGATCGAGGGGCGACCGGGTCGGGGCCGCTGCAC GCCAAGGGCGAAGGCCGATCCGGGCCCCACTTCGCCCCGGCGGCTCGCCGCGCCCACCCGCTCCGCGCCG AGGGCTGGAGGATGCGTTCCCTGGGGTCCGGACTTATGAAAATATGCATCAGTTTAATACTGTCTGTGGA ATTCATGAGATGGAAGCATAGGTCAAAGCTGTTTGGAGAAAATCAGAGGTACAGTTTTTATCTAGCCACA TCTTTGGAGGGAGTCGTAAGAAAAGCAGGTGGAAGTTGAGGTCATTGTCAGTTGCTGGCGATCTTTTACA AGAAAATCCTCACTGGATGGATAGTCATTTTAAATGGTGGAGGTAGCAGAAACAATATTAAAGGGTGACG TACAAGGAAACATACCAATTGACCATGGACTACGCTATCCTTTTCATACGATTATGGGAACCCAAATGGT CTCACATTCTCTCGTGTACAGGACAGACTCGGGAGTGTGGGCCACGGGCACTGGAGAGAATTCAGATCGC ACCGGAAGTTCAAATGGGTATACCTTACCCAGGAACCTGGCTTTTTAATGGCATGGCAGGCCTGCAATGG CTATACACAGTCACATGGCATGCGTTGCTCTAAGAGTGCCGGAACCATCTCGGTGAGTAAGATGATATGC GAGTACGCGCGAATCCGATTAGTTGACAGATGAAGCATGAGCCAGGCCAGGTAAAGGCCG >SC323635 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
GWTGGMTMGCGACTATTGCYAACAGATTCAGATGGTCCGGCAAKTTGGTAAAGGCCGATATGGAGAAGTA TGGATGGGSAAATGGCGTGGCGAAAAAGTGGCGGTGATGGTATTCTTTACCACTGAAGAAGCCAGCTGGT TTCGAGAAACAGAAATCTACCAAACTGTGCTAATGCGCCATGAAAACATACTTGGTTTCATAGCGGCAGA CATTAAAGGTACAGGTTCCTGGACTCAGCTCTATTTGATTACTGA |
| Restriction Sites | Please inquire |
| ACCN | NM_004329 |
| Insert Size | 3370 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004329.2, NP_004320.2 |
| RefSeq Size | 3631 bp |
| RefSeq ORF | 1599 bp |
| Locus ID | 657 |
| UniProt ID | P36894 |
| Domains | Activin_recp, pkinase, TyrKc, S_TKc, GS |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Transmembrane |
| Protein Pathways | Cytokine-cytokine receptor interaction, TGF-beta signaling pathway |
| Gene Summary | The bone morphogenetic protein (BMP) receptors are a family of transmembrane serine/threonine kinases that include the type I receptors BMPR1A and BMPR1B and the type II receptor BMPR2. These receptors are also closely related to the activin receptors, ACVR1 and ACVR2. The ligands of these receptors are members of the TGF-beta superfamily. TGF-betas and activins transduce their signals through the formation of heteromeric complexes with 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206048 | BMPR1A (Myc-DDK-tagged)-Human bone morphogenetic protein receptor, type IA (BMPR1A) |
CNY 3,960.00 |
|
| RC206048L1 | Lenti ORF clone of Human bone morphogenetic protein receptor, type IA (BMPR1A), Myc-DDK-tagged |
CNY 6,360.00 |
|
| RC206048L2 | Lenti ORF clone of Human bone morphogenetic protein receptor, type IA (BMPR1A), mGFP tagged |
CNY 5,890.00 |
|
| RC206048L3 | Lenti ORF clone of Human bone morphogenetic protein receptor, type IA (BMPR1A), Myc-DDK-tagged |
CNY 6,360.00 |
|
| RC206048L4 | Lenti ORF clone of Human bone morphogenetic protein receptor, type IA (BMPR1A), mGFP tagged |
CNY 6,360.00 |
|
| RG206048 | BMPR1A (tGFP-tagged) - Human bone morphogenetic protein receptor, type IA (BMPR1A) |
CNY 5,560.00 |
|
| SC127222 | BMPR1A (untagged)-Human bone morphogenetic protein receptor, type IA (BMPR1A) |
CNY 3,968.00 |
