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STK39 (NM_013233) Human Untagged Clone
CAT＃: SC323657
STK39 (untagged)-Kinase deficient mutant (K94M) of Human serine threonine kinase 39 (STE20/SPS1 homolog, yeast) (STK39)
CNY 4,080.00
CNY 8,740.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DCHT; PASK; SPAK |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_013233, the custom clone sequence may differ by one or more nucleotides
ATGGCGGAGCCGAGCGGCTCGCCCGTGCACGTCCAGCTTCCCCAGCAGGCGGCCCCGGTGACAGCGGCGG CGGCGGCGGCCCCGGCGGCCGCGACAGCAGCGCCGGCCCCGGCAGCTCCCGCGGCCCCGGCCCCGGCCCC GGCCCCGGCGGCACAGGCTGTCGGCTGGCCCATCTGCAGGGACGCGTACGAGCTGCAGGAGGTTATCGGC AGTGGAGCTACTGCTGTGGTTCAGGCAGCCCTATGCAAACCCAGGCAAGAACGTGTAGCAATAAAACGGA TCAACTTGGAAAAATGCCAGACCAGTATGGATGAACTATTAAAAGAAATTCAAGCCATGAGTCAGTGCAG CCATCCCAACGTAGTGACCTATTACACCTCTTTTGTGGTCAAAGATGAACTTTGGCTGGTCATGAAATTA CTAAGTGGAGGTTCAATGTTGGATATCATAAAATACATTGTCAACCGAGGAGAACACAAGAATGGAGTTC TGGAAGAGGCAATAATAGCAACAATTCTTAAAGAGGTTTTGGAAGGCTTAGACTATCTACACAGAAACGG TCAGATTCACAGGGATTTGAAAGCTGGTAATATTCTTCTGGGTGAGGATGGTTCAGTACAAATAGCAGAT TTTGGGGTAAGTGCGTTCCTAGCAACAGGGGGTGATGTTACCCGAAATAAAGTAAGAAAAACATTCGTTG GCACCCCATGTTGGATGGCTCCTGAAGTCATGGAACAGGTGAGAGGCTATGACTTCAAGGCTGACATGTG GAGTTTTGGAATAACTGCCATTGAATTAGCAACAGGAGCAGCGCCTTATCACAAATATCCTCCCATGAAA GTGTTAATGTTGACTTTGCAAAATGATCCACCCACTTTGGAAACAGGGGTAGAGGATAAAGAAATGATGA AAAAGTACGGCAAGTCCTTTAGAAAATTACTTTCACTGTGTCTTCAGAAAGATCCTTCCAAAAGGCCCAC AGCAGCAGAACTTTTAAAATGCAAATTCTTCCAGAAAGCCAAGAACAGAGAGTACCTGATTGAGAAGCTG CTTACAAGAACACCAGACATAGCCCAAAGAGCCAAAAAGGTAAGAAGAGTTCCTGGGTCAAGTGGTCACC TTCATAAAACCGAAGACGGGGACTGGGAGTGGAGTGACGACGAGATGGATGAGAAGAGCGAAGAAGGGAA AGCAGCTTTTTCTCAGGAAAAGTCACGAAGAGTAAAAGAAGAAAATCCAGAGATTGCAGTGAGTGCCAGC ACCATCCCCGAACAAATACAGTCCCTCTCTGTGCACGACTCTCAGGGCCCACCCAATGCTAATGAAGACT ACAGAGAAGCTTCTTCTTGTGCCGTGAACCTCGTTTTGAGATTAAGAAACTCCAGAAAGGAACTTAATGA CATACGATTTGAGTTTACTCCAGGAAGAGATACAGCAGATGGTGTATCTCAGGAGCTCTTCTCTGCTGGC TTGGTGGATGGTCACGATGTAGTTATAGTGGCTGCTAATTTACAGAAGATTGTAGATGATCCCAAAGCTT TAAAAACATTGACATTTAAGTTGGCTTCTGGCTGTGATGGGTCGGAGATTCCTGATGAAGTGAAGCTGAT TGGGTTTGCTCAGTTGAGTGTCAGCTGA >OriGene 5' read for mutant NM_013233 unedited
CCCGCCCGTTATCAGCAAAGGGCGGTAGGCGCTGTACGGCTGTGGAGGTCTATATAAGCAGAGCTCGTTT AGTGAACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTCCTAGCT GGCTTCGGCGGGGACGGCGGCGGCGGCGGCGGCGGCGGCGGCGGGCGGGGAGGGCGTGCGCCGGCCGAGA GGTGTCGGCGGCGAGGCAAAGGAAGTTTCAAGTGGAAGGTCGTCCGTCGGCCGGCGCGTCCTCCTGCTCT CCTCCGCAGCATCATGGCGGAGCCGAGCGGCTCGCCCGTGCACGTCCAGCTTTCCCCAGCAGGCGGGCCC CGGTGACAGCGGGCGGGCGGGCGGCGGCCCCGGCCCCGGCCCCGGCGGGCACAGGCTGTCGGCTTGGCCC TCCTGCAGGGACGCGTACGAGCTGCAGGAGGGTTATCGGCAGTGGAACCTACTGGTGGTGGTTCAGGCAG CCCAATGCCAAACCCAGGCAAAAAGTTGTACCATTAAGGCGGATCAACTTGAAAAATGCCGAACCAGTAG GGATAAACTATAAAAGAAATTCACCATTGATTCATGGGCACCCCTCCCAACGGTGGACCTATTACCCCTT TTTTGTTGTCAAATAAAATTTTGGCGTGGACATGAATTTCAAGGGGGGGTCAGTGTGATCTAAAACACTG TGACCCCGAGAACACCAAGGATTCGAGAAAAGAAAAAAACAATCTCTAAGTGTTTGAGCGCAACACTATC ACAACGCGCATCACAGAGTTAGACTGTGTATCTCTGTGGATGATGTAAAAAAAATTTTG >SC323657 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
AAAACCAGSACKMGAGTWCGGCAGTGGAGCTACTGCTGTGGTTCAGGCAGCCCTATGCAAACCCAGGCAA GAACGTGTAGCAATAATGCGGATCAACTTGGAAAAATGCCAGACCAGTATGGATGAACTATTAAAAGAAA TTCAAGCCATGAGTCAGTGCAGCCATCCCAACGTAGTGACCTATTACACCTCTTTTGTGGTCAAAGATGA ACTTTGGCTGGTCATGAAATTACTAAGTGGAGGTTCAATGTTGGA |
| Restriction Sites | Please inquire |
| ACCN | NM_013233 |
| Insert Size | 3160 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_013233.1, NP_037365.1 |
| RefSeq Size | 3293 bp |
| RefSeq ORF | 1644 bp |
| Locus ID | 27347 |
| UniProt ID | Q9UEW8 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene encodes a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223981 | STK39 (Myc-DDK-tagged)-Human serine threonine kinase 39 (STK39) |
CNY 4,072.00 |
|
| RC223981L1 | Lenti ORF clone of Human serine threonine kinase 39 (STK39), Myc-DDK-tagged |
CNY 6,472.00 |
|
| RC223981L2 | Lenti ORF clone of Human serine threonine kinase 39 (STK39), mGFP tagged |
CNY 6,460.00 |
|
| RC223981L3 | Lenti ORF clone of Human serine threonine kinase 39 (STK39), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC223981L4 | Lenti ORF clone of Human serine threonine kinase 39 (STK39), mGFP tagged |
CNY 6,472.00 |
|
| RG223981 | STK39 (tGFP-tagged) - Human serine threonine kinase 39 (STK39) |
CNY 5,672.00 |
|
| SC115335 | STK39 (untagged)-Human serine threonine kinase 39 (STK39) |
CNY 4,080.00 |
