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ADCK4 (COQ8B) (NM_024876) Human Untagged Clone
CAT＃: SC323680
ADCK4 (untagged)-Kinase deficient mutant (K237M) of Human aarF domain containing kinase 4 (ADCK4), transcript variant 1
CNY 2,400.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ADCK4; NPHS9 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_024876, the custom clone sequence may differ by one or more nucleotides
ATGTGGCTGAAGGTGGGGGGCCTACTTCGGGGGACCGGTGGACAGCTGGGCCAGACTGTTGGTTGGCCTT GTGGGGCCCTGGGGCCTGGGCCCCACCGCTGGGGACCATGTGGAGGTTCTTGGGCCCAAAAGTTTTACCA GGATGGGCCTGGGAGAGGCCTGGGTGAGGAGGACATTCGCAGGGCACGGGAGGCCCGTCCCAGGAAGACA CCCCGGCCCCAGCTGAGTGACCGCTCTCGAGAACGCAAGGTGCCTGCCTCCCGCATCAGCCGCTTGGCCA ACTTTGGGGGACTGGCTGTGGGCTTGGGGCTAGGAGTACTGGCCGAGATGGCTAAGAAGTCCATGCCAGG AGGTCGTCTGCAGTCAGAGGGTGGTTCTGGGCTGGACTCCAGCCCCTTCCTGTCGGAGGCCAATGCCGAG CGGATTGTGCAGACCTTATGTACAGTTCGAGGGGCCGCCCTCAAGGTTGGCCAGATGCTCAGCATCCAGG ACAACAGCTTCATCAGCCCTCAGCTGCAGCACATCTTTGAGCGGGTCCGCCAGAGCGCCGACTTCATGCC CCGCTGGCAGATGCTGAGAGTTCTTGAAGAGGAGCTCGGCAGGGACTGGCAGGCCAAGGTGGCCTCCTTG GAGGAGGTGCCCTTTGCCGCTGCCTCAATTGGGCAGGTGCACCAGGGCCTGCTGAGGGACGGGACGGAGG TGGCCGTGAAGATCCAGTACCCCGGCATAGCCCAGAGCATTCAGAGCGATGTCCAGAACCTGCTGGCGGT ACTCAAGATGAGCGCGGCCCTGCCCGCGGGCCTGTTTGCCGAGCAGAGCCTGCAGGCCTTGCAGCAGGAG CTGGCTTGGGAGTGTGACTACCGTCGTGAGGCGGCTTGTGCCCAGAATTTCAGGCAGCTGCTGGCAAATG ACCCCTTCTTCCGGGTCCCAGCCGTGGTTAAGGAGCTGTGCACGACACGGGTGCTGGGCATGGAGCTGGC TGGAGGGGTCCCCCTGGACCAGTGCCAGGGCCTAAGCCAGGACCTGCGGAACCAGATTTGCTTCCAGCTC CTGACGCTGTGTCTGCGGGAGCTGTTTGAGTTCCGATTCATGCAGACTGACCCCAACTGGGCCAACTTCC TGTATGATGCCTCCAGCCACCAGGTGACCCTGCTGGACTTTGGTGCAAGCCGGGAGTTTGGGACAGAGTT CACAGACCATTACATCGAGGTGGTGAAGGCTGCAGCTGATGGAGACAGAGACTGTGTCCTGCAGAAGTCC AGGGACCTCAAATTCCTCACAGGCTTTGAAACCAAGGCATTCTCCGACGCCCACGTGGAGGCAGTGATGA TCCTGGGGGAGCCTTTCGCCACCCAGGGCCCTTATGACTTTGGGTCGGGGGAAACGGCCCGCCGCATACA GGACCTCATCCCGGTGCTGCTGCGGCACCGGCTGTGTCCCCCACCCGAGGAGACCTATGCCCTGCACCGC AAGCTGGCAGGGGCTTTCCTGGCCTGTGCCCACCTCCGAGCCCACATCGCCTGCAGGGACCTCTTCCAGG ACACCTACCACCGCTACTGGGCCAGTCGCCAGCCAGACGCAGCCACTGCCGGCAGCCTCCCCACCAAAGG GGACTCCTGGGTGGATCCCTCATGA >OriGene 5' read for mutant NM_024876 unedited
CCCGCCCGTTCCAGCAAAGGGCGGTAGGCGTGTACGGTTGGGAGGTTCTATATAAGCAGAGCTCGTTTAG TGAACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTGAGCGGGA AACAGTTGGCAAGCAAGGCAATGTGGCTGAAGGTGGGGGGCCTACTTCGGGGGACCGGTGGACAGCTGGG CCAGACTGTTGGTTGGCCTTGTGGGGCCCTGGGGCCTGGGCCCCACCGCTGGGGACCATGTGGAGGTTCT TGGGCCCAAAAGTTTTACCAGGATGGGCCTGGGGAGAGGCCTGGGGTGAAGGAGGGACATTTCGGCAAGG GCACGGGAGAGGCCCGGTCCAAGGAAAGAACACCCCCGGCCCCCACTCTGATGTGACCCGCTCTCTCGAA AACGCCAAGTGTGCCGGCCCTCCCCCCATCAGCCCCCTTGCCACACCTTGGGGGGGACTGCCTGGGGGGC TGGGGGGCTAGAATTCCGGGCCCAAAAATGGCTAAAAATTCCCATGCCCGGGAGGCTGCCTTGCTTCCAA AGGGGGTTTTTGGGGCGGGACCCCCCCCCCTTTCCTTTTGAAAGGCCAAGGCCAAAACGGGATTTGGCCA ACCCCTATTTTCCTTTTAGGGGGCCCCCCCCCAAGGGTTGGCCCAAACCCCCCCCCCCCCCGGGGAACAC GAAACCCCAATTCTTGAACCCCTGGACCCCTTTTTTACCAAGCCCCTCCCCCAAAAAGGGCCAAATTTTT AAAAACCTACAGGGGGGCCCCCCCCCCCCTTGCTTTTTTTTTTCCCCTGAGTGCCCCGTGTGTCCCAAAA AAAAATTCTTTAACACCTCTTTGTACGAAAACATTTTTTAGGGGCGCTCCGACAACGCCTATTTTTCCCC CCGGGGAAGCATGAGTATTTTTATAAAAAAACACCTATGGAGCGCAGACCACCGAGCGTTCTCTCTTAAG TAGTGATTCTTCGTGTCTACTTCGTGTAGAGCACCCACCCCGCCGTGTAGATGCGCGCGCATGTAGACTT GCTT >SC323680 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CTGGAGCTGCTACTASGCTTAGCTGCTCAKSAKMSATGGGMRGKCACASGGCCTGCTGAGGGACGGGACG GAGGTGGCCGTGATGATCCAgTACCCCGGCATAGCCCAGAGCATTCAGAGCGATGTCCAGAACCTGCTGG CAGTACTCAAGATGAGCGCGGCCCTGCCCGCGGGCCTGTTTGCCGAGCAGAGCCTGCAGGCCTTGCASCA GGAGCTGGCTTGGGAGTGTGACTACCGTCRTGAGGCGGCTTGTGC |
| Restriction Sites | Please inquire |
| ACCN | NM_024876 |
| Insert Size | 2500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_024876.1, NP_079152.1 |
| RefSeq Size | 1949 bp |
| RefSeq ORF | 501 bp |
| Locus ID | 79934 |
| UniProt ID | Q96D53 |
| Domains | ABC1 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene encodes a protein with two copies of a domain found in protein kinases. The encoded protein has a complete protein kinase catalytic domain, and a truncated domain that contains only the active and binding sites of the protein kinase domain, however, it is not known whether the protein has any kinase activity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203700 | ADCK4 (Myc-DDK-tagged)-Human aarF domain containing kinase 4 (ADCK4), transcript variant 1 |
CNY 4,048.00 |
|
| RC203700L1 | Lenti ORF clone of Human aarF domain containing kinase 4 (ADCK4), transcript variant 1, Myc-DDK-tagged |
CNY 6,448.00 |
|
| RC203700L2 | Lenti ORF clone of Human aarF domain containing kinase 4 (ADCK4), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC203700L3 | Lenti ORF clone of Human aarF domain containing kinase 4 (ADCK4), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203700L4 | Lenti ORF clone of Human aarF domain containing kinase 4 (ADCK4), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG203700 | ADCK4 (tGFP-tagged) - Human aarF domain containing kinase 4 (ADCK4), transcript variant 1 |
CNY 5,648.00 |
|
| SC108116 | ADCK4 (untagged)-Human aarF domain containing kinase 4 (ADCK4), transcript variant 1 |
CNY 2,400.00 |
|
| SC322459 | ADCK4 (untagged)-Human aarF domain containing kinase 4 (ADCK4), transcript variant 1 |
CNY 4,056.00 |
