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RNase L (RNASEL) (NM_021133) Human Untagged Clone
CAT＃: SC323686
RNASEL (untagged)-Kinase deficient mutant (K392M) of Human ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent) (RNASEL)
CNY 8,152.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PRCA1; RNS4 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323686 sequence for NM_021133 edited (data generated by NextGen Sequencing)
ATGGAGAGCAGGGATCATAACAACCCCCAGGAGGGACCCACGTCCTCCAGCGGTAGAAGG GCTGCAGTGGAAGACAATCACTTGCTGATTAAAGCTGTTCAAAACGAAGATGTTGACCTG GTCCAGCAATTGCTGGAAGGTGGAGCCAATGTTAATTTCCAGGAAGAGGAAGGGGGCTGG ACACCTCTGCATAACGCAGTACAAATGAGCAGGGAGGACATTGTGGAACTTCTGCTTCGT CATGGTGCTGACCCTGTTCTGAGGAAGAAGAATGGGGCCACGCCTTTTATCCTCGCAGCG ATTGCGGGGAGCGTGAAGCTGCTGAAACTTTTCCTTTCTAAAGGAGCAGATGTCAATGAG TGTGATTTTTATGGCTTCACAGCCTTCATGGAAGCCGCTGTGTATGGTAAGGTCAAAGCC CTAAAATTCCTTTATAAGAGAGGAGCAAATGTGAATTTGAGGCGAAAGACAAAGGAGGAT CAAGAGCGGCTGAGGAAAGGAGGGGCCACAGCTCTCATGGACGCTGCTGAAAAAGGACAC GTAGAGGTCTTGAAGATTCTCCTTGATGAGATGGGGGCAGATGTAAACGCCTGTGACAAT ATGGGCAGAAATGCCTTGATCCATGCTCTCCTGAGCTCTGACGATAGTGATGTGGAGGCT ATTACGCATCTGCTGCTGGACCATGGGGCTGATGTCAATGTGAGGGGAGAAAGAGGGAAG ACTCCCCTGATCCTGGCAGTGGAGAAGAAGCACTTGGGTTTGGTGCAGAGGCTTCTGGAG CAAGAGCACATAGAGATTAATGACACAGACAGTGATGGCAAAACAGCACTGCTGCTTGCT GTTGAACTCAAACTGAAGAAAATCGCCGAGTTGCTGTGCAAACGTGGAGCCAGTACAGAT TGTGGGGATCTTGTTATGACAGCGAGGCGGAATTATGACCATTCCCTTGTGAAGGTTCTT CTCTCTCATGGAGCCAAAGAAGATTTTCACCCTCCTGCTGAAGACTGGAAGCCTCAGAGC TCACACTGGGGGGCAGCCCTGAAGGATCTCCACAGAATATACCGCCCTATGATTGGCAAA CTCAAGTTCTTTATTGATGAAAAATACAAAATTGCTGATACTTCAGAAGGAGGCATCTAC CTGGGGTTCTATGAGAAGCAAGAAGTAGCTGTGATGACGTTCTGTGAGGGCAGCCCACGT GCACAGCGGGAAGTCTCTTGTCTGCAAAGCAGCCGAGAGAACAGTCACTTGGTGACATTC TATGGGAGTGAGAGCCACAGGGGCCACTTGTTTGTGTGTGTCACCCTCTGTGAGCAGACT CTGGAAGCGTGTTTGGATGTGCACAGAGGGGAAGATGTGGAAAATGAGGAAGATGAATTT GCCCGAAATGTCCTGTCATCTATATTTAAGGCTGTTCAAGAACTACACTTGTCCTGTGGA TACACCCACCAGGATCTGCAACCACAAAACATCTTAATAGATTCTAAGAAAGCTGCTCAC CTGGCAGATTTTGATAAGAGCATCAAGTGGGCTGGAGATCCACAGGAAGTCAAGAGAGAT CTAGAGGACCTTGGACGGCTGGTCCTCTATGTGGTAAAGAAGGGAAGCATCTCATTTGAG GATCTGAAAGCTCAAAGTAATGAAGAGGTGGTTCAACTTTCTCCAGATGAGGAAACTAAG GACCTCATTCATCGTCTCTTCCATCCTGGGGAACATGTGAGGGACTGTCTGAGTGACCTG CTGGGTCATCCCTTCTTTTGGACTTGGGAGAGCCGCTATAGGACGCTTCGGAATGTGGGA AATGAATCCGACATCAAAACACGAAAATCTGAAAGTGAGATCCTCAGACTACTGCAACCT GGGCCTTCTGAACATTCCAAAATTTTTGACAAGTGGACGACTAAGATTAATGAATGTGTT ATGAAAAAAATGAATAAGTTTTATGAAAAAAGAGGCAATTTCTACCAGAACACTGTGGGT GATCTGCTAAAGTTCATCCGGAATTTGGGAGAACACATTGATGAAGAAAAGCATAAAAAG ATGAAATTAAAAATTGGAGACCCTTCCCTGTATTTTCAGAAGACATTTCCAGATCTGGTG ATCTATGTCTACACAAAACTACAGAACACAGAATATAGAAAGCATTTCCCCCAAACCCAC AGTCCAAACAAGCCTCAGTGTGATGGAGCTGGTGGGGCCAGTGGGTTGGCCAGCCCTGGG TGCTGA Clone variation with respect to NM_021133.3 1175 a=>t;1883 g=>t >OriGene 5' read for mutant NM_021133 unedited
CCCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGCTGCTCGGGCTGCAA GCAGTCTTCCAGGCTTTGCGGCTGCCAAAGGAATAATTGAGACGTCTGAGTTGAGCAGGTGGAATGTCAG AAGACTGAGAACATTGTTCCTTCTTCATACTGCTGCTCTGTTGCCAGAGAATCCCAATTTACACTCAAAG CTTCTTTGATTAAGTGCTAGGAGATAAATTTGCATTTTCTCAAGGAAAAGGCTAAAAGTGGTAGCAGGGT GGCATTTACCGTCATGGGAGAGCAGGGATCATAACAACCCCCCAGGAAGGGACCCACGTCCTTCCAGCGG TAGAAGGGCTGCAGTGGAAGACAATCACTTGCTGATTAAAGCTGGTTCAAAACGAAGAATGTTGACTTGT TCAGCATTGCTGGGAGGTGGAGCAAAGGTAAATTTCAGAAAAAGGAAGGGGCCTGGACCCCCCCCATTAA GGGATTCCAATGGGCGAGGGAGGACTTTGGAATTTTCCTCTGGCAAGGTGCCTGACCGGTTCTAGAGAAG AGATGGGCCACGCTTTTACTCTCACAGATTCGGGAGCCGTAACTCTCGAACCTTCCTTTAAGAGACAATG CAGAGGGAATTTAGGCCAACTTTGGACCCTGTGTTGAAGCAACCTAATCCTTAAAGCATGAATAGCCACA GGATCAGGTGAAAGGCCACCTAGCTCGAAGACTTAGCTGATCTGAATTGCAATACCGGTAATGCAATGCT TCGCTGCTTCAAGTGAGCTC >SC323686 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CWTGATTGGCACTCAGTTCTTTATTGATGAAAATACAAAATTGCTGATACTTCAGAAGGAGGCATCTACC TGGGGTTCTATGAGAAGCAAGAAGTAGCTGTGATGACGTTCTGTGAGGGCAGCCCACGTGCACAGCGGGA AGTCTCTTGTCTGCAAAGCAGCCGAGAGAACAGTCACTTGGTGACATTCTATGGGAGTGAGAGCCACAGG GGCCACTTGTTTGTGTGTGTCACCCTCTGTGAGCAGACTCTGGAA |
| Restriction Sites | Please inquire |
| ACCN | NM_021133 |
| Insert Size | 4700 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_021133.2, NP_066956.1 |
| RefSeq Size | 4166 bp |
| RefSeq ORF | 2226 bp |
| Locus ID | 6041 |
| UniProt ID | Q05823 |
| Domains | pkinase, ANK, S_TKc, PUG |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene encodes a component of the interferon-regulated 2-5A system that functions in the antiviral and antiproliferative roles of interferons. Mutations in this gene have been associated with predisposition to prostate cancer and this gene is a candidate for the hereditary prostate cancer 1 (HPC1) allele. [provided by RefSeq, Jul 2008] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214849 | RNASEL (Myc-DDK-tagged)-Human ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent) (RNASEL) |
CNY 5,432.00 |
|
| RC214849L1 | Lenti-ORF clone of RNASEL (Myc-DDK-tagged)-Human ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent) (RNASEL) |
CNY 5,890.00 |
|
| RC214849L2 | Lenti-ORF clone of RNASEL (mGFP-tagged)-Human ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent) (RNASEL) |
CNY 7,832.00 |
|
| RC214849L3 | Lenti-ORF clone of RNASEL (Myc-DDK-tagged)-Human ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent) (RNASEL) |
CNY 7,832.00 |
|
| RC214849L4 | Lenti-ORF clone of RNASEL (mGFP-tagged)-Human ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent) (RNASEL) |
CNY 7,832.00 |
|
| RG214849 | RNASEL (tGFP-tagged) - Human ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent) (RNASEL) |
CNY 7,032.00 |
|
| SC112978 | RNASEL (untagged)-Human ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent) (RNASEL) |
CNY 8,152.00 |
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