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CDK15 (NM_139158) Human Untagged Clone
CAT＃: SC323695
CDK15 (untagged)-Kinase deficient mutant (K81M) of Human PFTAIRE protein kinase 2 (PFTK2)
CNY 3,656.00
CNY 6,270.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ALS2CR7; PFTAIRE2; PFTK2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323695 sequence for NM_139158 edited (data generated by NextGen Sequencing)
ATGACTTCATTTCACCCCAGGGGACTTCAAGCTGCCCGTGCCCAGAAGTTCAAGAGTAAA AGGCCACGGAGTAACAGTGATTGTTTTCAGGAAGAGGATCTGAGGCAGGGTTTTCAGTGG AGGAAGAGCCTCCCTTTTGGGGCAGCCTCATCTTACTTGAACTTGGAGAAGCTGGGTGAA GGCTCTTATGCGACAGTTTACAAGGGGATTAGCAGAATAAATGGACAACTAGTGGCTTTA AWRGTCATCAGCATGAATGCAGAGGAAGGAGTCCCATTTACAGCTATCCGAGAAGCTTCT CTCCTGAAGGGTTTGAAACATGCCAATATTGTGCTCCTGCATGACATAATCCACACCAAA GAGACACTGACATTCGTTTTTGAATACATGCACACAGACCTGGCCCAGTATATGTCTCAG CATCCAGGAGGGCTTCATCCTCATAATGTCAGACTTTTCATGTTTCAACTTTTGCGGGGC CTGGCGTACATCCACCACCAACACGTTCTTCACAGGGACCTGAAACCTCAGAACTTACTC ATCAGTCACCTGGGAGAGCTCAAACTGGCTGATTTTGGTCTTGCCCGGGCCAAGTCCATT CCCAGCCAGACATACTCTTCAGAAGTCGTGACCCTCTGGTACCGGCCCCCTGATGCTTTG CTGGGAGCCACTGAATATTCCTCTGAGCTGGACATATGGGGTGCAGGCTGCATCTTTATT GAAATGTTCCAGGGTCAACCTTTGTTTCCTGGGGTTTCCAACATCCTTGAACAGCTGGAG AAAATCTGGGAGGTGCTGGGAGTCCCTACAGAGGATACTTGGCCGGGAGTCTCCAAGCTA CCTAACTACAATCCAGAATGGTTCCCACTGCCTACGCCTCGAAGCCTTCATGTTGTCTGG AACAGGCTGGGCAGGGTTCCTGAAGCTGAAGACCTGGCCTCCCAGATGCTAAAAGGCTTT CCCAGAGACCGCGTCTCCGCCCAGGAAGCACTTGTTCATGATTATTTCAGCGCCCTGCCA TCTCAGCTGTACCAGCTTCCTGATGAGGAGTCTTTGTTTACAGTTTCAGGAGTGAGGCTA AAGCCAGAAATGTGTGACCTTTTGGCCTCCTACCAGAAAGGTCACCACCCAGCCCAGTTT AGCAAATGCTGGTGA Clone variation with respect to NM_139158.1 242 a=>w;243 a=>r >OriGene 5' read for mutant NM_139158 unedited
ACCGCCGTCTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGAGGAGTCCCCAGAG AGGCAGGAGGAAAACCAAGGAAGTGCCCACTTCACAGAATCAAGGAGGCTTTCAAAAAGGGTGGCATGAT TATGGTGTTAAATGCCACCAAAGAGTAAAGCAAGGACTCTTGCTCTGTCACCCAGTCTGAAGTGCTGTGG CGCAACCATGGCTCTATGCAGCCTTGACCTCCTGAACTCAAGCAATTGTCCCTGCCTCAGCCTTCCCAGT TGCTGGGACCGCGGCTGCCTCGTTTTCAGTTTGTGCACATCTGATTGCCAAGCAATCCCGGTCTGAGGTG GAAGTTTCTTGCACTGATAAGGAAAAACTGCTGAGGTTGTGAGGCTGCTCCAAGGCCGAAGCATCATCCT ACCGGACTAAAAAGAAGGCTCATGGTTCCAGAGACTTCATTTACCCAAGGGAATCTTAAGCCGCGCCCTG TGCCAGAAATTTCAGAGAGTAAAAGGCACAGGGATATAACGTGTGATGTGTTTTCGAGAAGAGTCTCGAG GCACAGGTTTCAGTGGGGCGGAAGGGGAGACCCTCCTTTGGGCGCGCCCTCATCTCTTTAGATTTGGAAG ACTGTTGAAGCGCTCTTGCGCAGTTTCACAGGGGTATCACAGAATAGGCACTCTGTGTCTATAGTGTCTC ACGATGCCAGAGAGTCTCTATACTTCCAAGCTTCTCTCGAGGTTGAATGCACATGGTGCTGCGTGGATAT ACCACAGACCTCGATCTCGGTTGATCGACGAACTGCAAATTCATCAGGCTTCTATGGCACTAGTGACATC GGCTGCATCCACAGCGTAAGAACGAACTGACATCCCTA >SC323695 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CTCGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGAGGAGTCCCCAGAGAGGCAGG AGGAAAACCAAGGAAGTGCCCACTTCACAGAATCAAGGAGGCTTTCAAAAAGGGTGGCATGATTATGGTG TTAAATGCCACCAAAGAGTAAAGCAAGGATCTTGCTCTGTCACCC |
| Restriction Sites | Please inquire |
| ACCN | NM_139158 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_139158.1, NP_631897.1 |
| RefSeq Size | 1534 bp |
| RefSeq ORF | 1155 bp |
| Locus ID | 65061 |
| UniProt ID | Q96Q40 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | Serine/threonine-protein kinase that acts like an antiapoptotic protein that counters TRAIL/TNFSF10-induced apoptosis by inducing phosphorylation of BIRC5 at 'Thr-34'.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) has an alternate in-frame 5' exon and initiates translation at an upstream AUG compared to variant 1. This results in a shorter protein (isoform 3) with a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219623 | CDK15 (Myc-DDK-tagged)-Human cyclin-dependent kinase 15 (CDK15) |
CNY 3,656.00 |
|
| RC219623L1 | Lenti ORF clone of Human cyclin-dependent kinase 15 (CDK15), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC219623L2 | Lenti ORF clone of Human cyclin-dependent kinase 15 (CDK15), mGFP tagged |
CNY 5,890.00 |
|
| RC219623L3 | Lenti ORF clone of Human cyclin-dependent kinase 15 (CDK15), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC219623L4 | Lenti ORF clone of Human cyclin-dependent kinase 15 (CDK15), mGFP tagged |
CNY 5,890.00 |
|
| RG219623 | CDK15 (tGFP-tagged) - Human cyclin-dependent kinase 15 (CDK15) |
CNY 5,256.00 |
|
| SC120591 | CDK15 (untagged)-Human cyclin-dependent kinase 15 (CDK15) |
CNY 3,656.00 |
