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PAK3 (NM_002578) Human Untagged Clone
CAT＃: SC323702
PAK3 (untagged)-Kinase deficient mutant (V261M) of Human p21 protein (Cdc42/Rac)-activated kinase 3 (PAK3), transcript variant 2
CNY 8,740.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ARA; beta-PAK; bPAK; MRX30; MRX47; OPHN3; PAK-3; PAK3beta |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_002578 |
| Insert Size | 1635 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002578.2 |
| RefSeq Size | 2516 bp |
| RefSeq ORF | 1635 bp |
| Locus ID | 5063 |
| UniProt ID | O75914 |
| Domains | PBD, pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase, Stem cell - Pluripotency |
| Protein Pathways | Axon guidance, ErbB signaling pathway, Focal adhesion, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway |
| MW | 60.7 kDa |
| Gene Summary | The protein encoded by this gene is a serine-threonine kinase and forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1. This protein may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Defects in this gene are the cause of a non-syndromic form of X-linked intellectual disability. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2017] Transcript Variant: This variant (2) represents use of an alternate promoter compared to variant 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222595 | PAK3 (Myc-DDK-tagged)-Human p21 protein (Cdc42/Rac)-activated kinase 3 (PAK3), transcript variant 2 |
CNY 6,072.00 |
|
| RC222595L3 | Lenti-ORF clone of PAK3 (Myc-DDK-tagged)-Human p21 protein (Cdc42/Rac)-activated kinase 3 (PAK3), transcript variant 2 |
CNY 6,650.00 |
|
| RC222595L4 | Lenti-ORF clone of PAK3 (mGFP-tagged)-Human p21 protein (Cdc42/Rac)-activated kinase 3 (PAK3), transcript variant 2 |
CNY 6,650.00 |
|
| RG222595 | PAK3 (tGFP-tagged) - Human p21 protein (Cdc42/Rac)-activated kinase 3 (PAK3), transcript variant 2 |
CNY 7,672.00 |
|
| SC316402 | PAK3 (untagged)-Human p21 protein (Cdc42/Rac)-activated kinase 3 (PAK3), transcript variant 2 |
CNY 8,740.00 |
