United States
Germany
Japan
United Kingdom
China
NIM1K (NM_153361) Human Untagged Clone
CAT＃: SC323705
NIM1 (untagged)-Kinase deficient mutant (K103M) of Human serine/threonine-protein kinase NIM1 (MGC42105)
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | NIM1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_153361, the custom clone sequence may differ by one or more nucleotides
ATGACTGCAGTGTATATGAATGGAGGTGGCCTGGTGAACCCCCACTATGCCCGGTGGGATCGGCGCGACA GTGTAGAAAGTGGCTGTCAGACCGAGAGTAGCAAGGAGGGTGAGGAGGGACAGCCCCGCCAGCTGACGCC CTTCGAGAAACTGACACAGGACATGTCCCAGGATGAGAAGGTGGTGAGGGAGATCACGCTGGGGAAACGG ATAGGCTTCTACCGAATTCGAGGGGAAATCGGAAGTGGAAACTTCTCCCAAGTGAAGCTTGGGATTCACT CCCTAACCAAAGAAAAGGTGGCCATTAAGATCCTGGACAAGACCAAGTTAGACCAGAAAACCCAGAGGCT ACTATCCCGAGAAATCTCCAGCATGGAAAAGCTGCACCATCCCAACATCATCCGCCTTTACGAAGTGGTG GAGACCCTATCCAAGCTGCACTTGGTGATGGAGTATGCAGGGGGTGGGGAGCTCTTCGGAAAAATTAGCA CTGAGGGGAAGCTCTCTGAACCAGAAAGCAAGCTCATCTTCTCCCAGATTGTGTCTGCCGTGAAGCACAT GCATGAAAACCAAATTATTCATAGAGATCTGAAAGCAGAAAATGTATTCTATACCAGTAATACTTGTGTG AAGGTGGGCGATTTTGGATTCAGCACAGTAAGCAAAAAAGGTGAAATGCTGAACACTTTCTGTGGGTCTC CTCCCTACGCTGCGCCTGAACTCTTCCGGGACGAGCACTACATCGGCATTTACGTGGATATCTGGGCCTT GGGGGTGCTTTTGTACTTCATGGTGACTGGCACCATGCCATTTCGGGCAGAAACCGTGGCCAAACTAAAA AAGAGCATCCTCGAGGGCACATACAGTGTACCGCCGCACGTGTCAGAGCCCTGCCACCGACTCATCCGAG GAGTCCTTCAGCAGATCCCCACGGAGAGGTACGGAATCGACTGCATCATGAATGATGAATGGATGCAAGG GGTGCCATACCCTACACCTTTGGAACCTTTCCAACTGGATCCCAAACATTTGTCGGAAACCAGCACTCTC AAGGAAGAAGAAAATGAGGTCAAAAGCACTTTAGAACATTTGGGCATTACAGAAGAGCATATTCGAAATA ACCAAGGGAGAGATGCTCGCAGCTCAATCACAGGGGTCTATAGAATTATTTTACATAGAGTCCAAAGGAA GAAGGCTTTGGAAAGTGTCCCAGTCATGATGCTACCAGACCCTAAAGAAAGAGACCTCAAAAAAGGGTCC CGTGTCTACAGAGGGATAAGACACACATCCAAATTTTGCTCGATTTTATAA >OriGene 5' read for NM_153361 unedited
TCCGCCCGTCGAGCAACGGGCGGTAGGCGCTGTACGGATGGGAGGTCTATATAAGCAGAGCTCGTTTAGT GAACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTGCCGGTCAGG TCGGCCGCCCCTGACAGCTCCGGGAGCCTCAAGCGCGACAGCGGCGCCCTCACCTCGGGACATCCACACA CCGACCGCTCCTGCTCCAGAGGCAACAACCCAGCGCGCCTAGCCTGGCGCCGTGCAGCGAAGCCCAAGAG CTGGCCTCGCCACGAAGAGATGGGGTCTCACTGTGTTGCCTAGGCTGGTCTTGAGCTCCTGGACTCAAAC CATCCACCCGTCTCGGTCTCCCAAAGTGCTGGGATTATAGGTGTGAGCCACCACAACCAGCCTATTTTCT TTTTAAAGGTTGAACCAGCCAAATTTTCGAGACAGCTCACGGCTTAGAGGAAGGTTCATCTAAATAAAGG CCGGCTAAAGTGACATTGCAGGGATTAAATCCTTCTTTGGCTGCCTGTGTGACCAGAAGGCTTATTTGCA AGTTTCTTCTTTCCTGGGGTCCAGATTATTAGGTCTCCAGCGCCCTGCAGCTTGACAGAAAGAGAAGCAT GAAATGAAGGTCAGAGATGAGATCCCGCAGCAGGGACGTGGGGGCCTCCCAGGGGCATTTACGCACCAGA GTGCAAGATTCTCTGGCCATCAAGGGAAATAGCAAACAGAAGCCTTTGTCCTGGGGCACAGCCACCTACC ACAAAGCATCAGACTCCACGTCTGGCCAGAAAGTTCCTGGAGTCCCATCAGCCAGTGGGTATGTACATGT GCCTAATTGTACAGCTAGAGCCTGCAAGTTCAACGTGAGGGAGGTGGCAAATGTCTGAAGTGAAGCGAGC AGCTCTGGCTGGCTGGCAGAACTCACCTACCACGTCACTGGCTTCCTTCTCACTTAGAGGC >SC323705 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TACTCTTCGCTAGWGGAGACGTGAGCCCCCGTGGACGATGACTGCAGTGTATATGAATGGAGGTGGCCTG GTGAACCCCCACTATGCCCGGTGGGATCGGCGCGACAGTGTAGAAAGTGGCTGTCAGACCGAGAGTAGCA AGGAGGGTGAGGAGGGACAGCCCCGCCAGCTGACGCCCTTCGAGAAACTGACACAGGACATGTCCCAGGA TGAGAAGGTGGTGAGGGAGATCACGCTGGGGAAACGGATAGGCTT |
| Restriction Sites | Please inquire |
| ACCN | NM_153361 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_153361.2, NP_699192.1 |
| RefSeq Size | 2326 bp |
| RefSeq ORF | 1311 bp |
| Locus ID | 167359 |
| UniProt ID | Q8IY84 |
| Protein Families | Druggable Genome, Protein Kinase |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207250 | NIM1 (Myc-DDK-tagged)-Human serine/threonine-protein kinase NIM1 (NIM1) |
CNY 3,656.00 |
|
| RC207250L1 | Lenti ORF clone of Human serine/threonine-protein kinase NIM1 (NIM1), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC207250L2 | Lenti ORF clone of Human serine/threonine-protein kinase NIM1 (NIM1), mGFP tagged |
CNY 5,890.00 |
|
| RC207250L3 | Lenti ORF clone of Human serine/threonine-protein kinase NIM1 (NIM1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207250L4 | Lenti ORF clone of Human serine/threonine-protein kinase NIM1 (NIM1), mGFP tagged |
CNY 5,890.00 |
|
| RG207250 | NIM1 (tGFP-tagged) - Human serine/threonine-protein kinase NIM1 (NIM1) |
CNY 4,370.00 |
|
| SC100346 | NIM1 (untagged)-Human serine/threonine-protein kinase NIM1 (NIM1) |
CNY 3,656.00 |
