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RAD18 (NM_020165) Human Untagged Clone
CAT＃: SC323786
RAD18 (untagged)-Human RAD18 homolog (S. cerevisiae) (RAD18)
CNY 3,656.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | RNF73 |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_020165.2
GTGTTGGAGCGCCGGAGGCTAGTGGGTGGCTGACCCCCAGCATCCTCGAGAGCGACCATG
GACTCCCTGGCCGAGTCTCGGTGGCCTCCGGGCCTGGCAGTCATGAAGACAATAGATGAT TTGCTGCGGTGTGGAATTTGCTTCGAGTATTTCAACATTGCAATGATAATACCTCAGTGT TCACATAACTACTGCTCTCTCTGTATAAGAAAATTTCTGTCCTATAAAACTCAGTGTCCA ACTTGCTGTGTGACTGTCACAGAGCCGGATCTGAAAAATAACCGCATATTAGATGAACTG GTAAAAAGCTTGAATTTTGCACGGAATCATCTGCTGCAGTTTGCTTTAGAGTCACCAGCC AAATCTCCTGCTTCTTCCTCTTCAAAGAATCTTGCTGTCAAAGTATATACTCCTGTAGCC TCCAGACAGTCTTTAAAGCAGGGGAGCAGGTTAATGGATAATTTCTTGATCAGAGAAATG AGTGGTTCTACATCAGAGTTGTTGATAAAAGAAAATAAAAGCAAATTCAGCCCTCAAAAA GAGGCGAGCCCTGCTGCAAAGACCAAAGAGACACGTTCTGTAGAAGAGATCGCTCCAGAT CCCTCAGAGGCTAAGCGTCCTGAGCCACCCTCGACATCCACTTTGAAACAAGTTACTAAA GTGGATTGTCCTGTTTGCGGGGTTAACATTCCAGAAAGTCACATTAATAAGCATTTAGAC AGCTGTTTATCACGCGAAGAGAAGAAGGAAAGCCTCAGAAGTTCTGTTCACAAAAGGAAG CCGCTGCCCAAAACTGTATATAATTTGCTCTCTGATCGTGATTTAAAGAAAAAGCTAAAA GAGCATGGATTATCTATTCAAGGAAATAAACAACAGCTCATTAAAAGGCACCAAGAATTT GTACACATGTACAATGCCCAATGCGATGCTTTGCATCCTAAATCAGCTGCTGAAATAGTT CAAGAAATCGAAAATATAGAGAAGACTAGGATGCGTCTTGAAGCTAGTAAACTCAATGAA AGTGTAATGGTTTTTACAAAGGACCAAACAGAAAAGGAAATAGATGAAATCCACAGTAAA TATCGTAAAAAACATAAGAGTGAATTTCAGCTTCTGGTGGATCAGGCTAGAAAAGGATAC AAGAAAATTGCTGGAATGTCACAAAAAACAGTAACAATAACAAAAGAAGATGAATCTACA GAAAAGCTATCTTCTGTATGCATGGGACAGGAAGATAATATGACCTCAGTAACAAACCAC TTTTCTCAATCAAAGCTGGACTCCCCAGAGGAATTGGAACCTGACAGAGAAGAGGATTCT TCTAGCTGTATTGATATTCAAGAAGTTCTTTCTTCATCAGAATCAGATTCATGCAATAGT TCCAGTTCAGACATCATAAGAGATCTTTTAGAAGAAGAGGAAGCCTGGGAAGCATCACAT AAAAACGATCTTCAAGACACAGAAATAAGTCCAAGACAGAATCGCCGCACAAGAGCCGCT GAAAGTGCTGAGATTGAACCAAGAAACAAGCGTAATAGGAATTAATGTGGGCTTTTGCTG ACTTTTCAAATGCATTGATTAGAATACCGTACTTTTGGTTGCCACAGATAGATTTTCTAT TTATAAATGCCCAAGGAAAGATGCTAAATTCTAAATATTACGGTTAGCTGATATTCATTC TTTTCTGCTTTTCCAGAGGGGAAAAATGTTACAAAATATCCTTACTTGGTCAGTTGCCTC CTGCCTCTAAAACATCTCTCTCTAAAAATACTGACATTTCACACAGGTACCAGCTTTGCA GAGGAGGTAGACTCTTTGGCACTTTGGCACAGGGATTTGGTTTGGTTTGGTTTGGTTTGA TAATTAAATTTCAGAATGTCAAAAAAAAAAAAAAAA |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_020165 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020165.2, NP_064550.2 |
| RefSeq Size | 3053 bp |
| RefSeq ORF | 1488 bp |
| Locus ID | 56852 |
| UniProt ID | Q9NS91 |
| Domains | RING, SAP, ZnF_Rad18 |
| Protein Families | Druggable Genome |
| Gene Summary | The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Evidence for Ubiquitin-Regulated Nuclear and Subnuclear Trafficking among Paramyxovirinae Matrix Proteins
,Pentecost, M;Vashisht, AA;Lester, T;Voros, T;Beaty, SM;Park, A;Wang, YE;Yun, TE;Freiberg, AN;Wohlschlegel, JA;Lee, B;,
PLoS Pathog.
,PubMed ID 25782006
[RAD18]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201700 | RAD18 (Myc-DDK-tagged)-Human RAD18 homolog (S. cerevisiae) (RAD18) |
CNY 3,656.00 |
|
| RC201700L1 | Lenti ORF clone of Human RAD18 homolog (S. cerevisiae) (RAD18), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC201700L2 | Lenti ORF clone of Human RAD18 homolog (S. cerevisiae) (RAD18), mGFP tagged |
CNY 6,056.00 |
|
| RC201700L3 | Lenti ORF clone of Human RAD18 homolog (S. cerevisiae) (RAD18), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC201700L4 | Lenti ORF clone of Human RAD18 homolog (S. cerevisiae) (RAD18), mGFP tagged |
CNY 6,056.00 |
|
| RG201700 | RAD18 (tGFP-tagged) - Human RAD18 homolog (S. cerevisiae) (RAD18) |
CNY 5,256.00 |
|
| SC108413 | RAD18 (untagged)-Human RAD18 homolog (S. cerevisiae) (RAD18) |
CNY 3,656.00 |
