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RAD18 (NM_020165) Human Untagged Clone
CAT＃: SC108413
RAD18 (untagged)-Human RAD18 homolog (S. cerevisiae) (RAD18)
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | RNF73 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC108413 sequence for NM_020165 edited (data generated by NextGen Sequencing)
ATGGACTCCCTGGCCGAGTCTCGGTGGCCTCCGGGCCTGGCAGTCATGAAGACAATAGAT GATTTGCTGCGGTGTGGAATTTGCTTCGAGTATTTCAACATTGCAATGATAATACCTCAG TGTTCACATAACTACTGCTCTCTCTGTATAAGAAAATTTCTGTCCTATAAAACTCAGTGT CCAACTTGCTGTGTGACTGTCACAGAGCCGGATCTGAAAAATAACCGCATATTAGATGAA CTGGTAAAAAGCTTGAATTTTGCACGGAATCATCTGCTGCAGTTTGCTTTAGAGTCACCA GCCAAATCTCCTGCTTCTTCCTCTTCAAAGAATCTTGCTGTCAAAGTATATACTCCTGTA GCCTCCAGACAGTCTTTAAAGCAGGGGAGCAGGTTAATGGATAATTTCTTGATCAGAGAA ATGAGTGGTTCTACATCAGAGTTGTTGATAAAAGAAAATAAAAGCAAATTCAGCCCTCAA AAAGAGGCGAGCCCTGCTGCAAAGACCAAAGAGACACGTTCTGTAGAAGAGATCGCTCCA GATCCCTCAGAGGCTAAGCGTCCTGAGCCACCCTCGACATCCACTTTGAAACAAGTTACT AAAGTGGATTGTCCTGTTTGCGGGGTTAACATTCCAGAAAGTCACATTAATAAGCATTTA GACAGCTGTTTATCACGCGAAGAGAAGAAGGAAAGCCTCAGAAGTTCTGTTCACAAAAGG AAGCCGCTGCCCAAAACTGTATATAATTTGCTCTCTGATCGTGATTTAAAGAAAAAGCTA AAAGAGCATGGATTATCTATTCAAGGAAATAAACAACAGCTCATTAAAAGGCACCAAGAA TTTGTACACATGTACAATGCCCAATGCGATGCTTTGCATCCTAAATCAGCTGCTGAAATA GTTCGAGAAATCGAAAATATAGAGAAGACTAGGATGCGTCTTGAAGCTAGTAAACTCAAT GAAAGTGTAATGGTTTTTACAAAGGACCAAACAGAAAAGGAAATAGATGAAATCCACAGT AAATATCGTAAAAAACATAAGAGTGAATTTCAGCTTCTGGTGGATCAGGCTAGAAAAGGA TACAAGAAAATTGCTGGAATGTCACAAAAAACAGTAACAATAACAAAAGAAGATGAATCT ACAGAAAAGCTATCTTCTGTATGCATGGGACAGGAAGATAATATGACCTCAGTAACAAAC CACTTTTCTCAATCAAAGCTGGACTCCCCAGAGGAATTGGAACCTGACAGAGAAGAGGAT TCTTCTAGCTGTATTGATATTCAAGAAGTTCTTTCTTCATCAGAATCAGATTCATGCAAT AGTTCCAGTTCAGACATCATAAGAGATCTTTTAGAAGAAGAGGAAGCCTGGGAAGCATCA CATAAAAACGATCTTCAAGACACAGAAATAAGTCCAAGACAGAATCGCCGCACAAGAGCC GCTGAAAGTGCTGAGATTGAACCAAGAAACAAGCGTAATAGGAATTAA Clone variation with respect to NM_020165.3 >OriGene 5' read for NM_020165 unedited
GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATTCGG CACGAGGGACCCCCAGCATCCTCGAGAGCGACCATGGACTCCCTGGCCGAGTCTCGGTGG CCTCCGGGCCTGGCAGTCATGAAGACAATAGATGATTTGCTGCGGTGTGGAATTTGCTTC GAGTATTTCAACATTGCAATGATAATACCTCAGTGTTCACATAACTACTGCTCTCTCTGT ATAAGAAAATTTCTGTCCTATAAAACTCAGTGTCCAACTTGCTGTGTGACTGTCACAGAG CCGGATCTGAAAAATAACCGCATATTAGATGAACTGGTAAAAAGCTTGAATTTTGCACGG AATCATCTGCTGCAGTTTGCTTTAGAGTCACCAGCCAAATCTCCTGCTTCTTCCTCTTCA AAGAATCTTGCTGTCAAAGTATATACTCCTGTAGCCTCCAGACAGTCTTTAAAGCAGGGG AGCAGGTTAATGGATAATTTCTTGATCAGAAAATGAGTGGTTCTACTCAGAGTTGTTGAT AAAGAAATAAAAGCAATTCACCCTCAAAAGAGCGAGCCCTGCTGAAAGACCAAGAGACAC GTTCTGAAAGAGATCGCTCAATCCTCAAGGCTAACGTCTGAGCCACCTCGACATCCTTGA ACAAGTACTAAGTGGATNNGTCTGTTGCGGGNTACATTCAAAAAGCCATTTATACATTAC CAGCTGNTATCACCGAAAGAAGAAGCCTAAAANTTTGTACAAAGAAGCCCTCCAAACTGT TTAATNGCTCTTGGACGGTTAAGAAACTAAAACTGGGTTTTCTTCAGAATACACGCTCTA AAGCCCAAATTTNCCCGCCAGGCAGGGAGGTTTCTC >OriGene 3' read for NM_020165 unedited
TCTCGCTGTACGACCCACTCTTAGCACTAGATAAGTAGTCATGNAAACAAACAGTTTTCA TTTTGACATAATTTCTGACAACTCAGTGACATAAATATATCCATGTGAGCTCCTATTTTG TTTGACATGCTCACTTACCTCACATCAGTTCTGCCGATAGATTTGGTAAGTGGTGCTTTT AGTTTCCTTAATGCACCCCTCAGGATTTCATGCAAATGAAATTATTATGACAAATTTTTC TATAGCCATTTCTAACACAGGGAACTCATAAAGAAATCAAATGTCTGCACTTCACTGTGA AAACACTAAACTCTCAGCCATAATGAGACTCCACACATTATCAGAAACCCCAAGGCATAC CAAAAAGACAAGGTCAACGGAGACAGCCTCTGGAAGGGGAGGTCATACCTGCCGCAACCT GGCCAACCATAAATCTCTAGTTTTAACATTGCCGCATTTACTATCATCTCATTTGAAAGC TGATGTGCTTGGGCTGTTTGATAACCCCAGGCGTGGACTCGTGCTATGCTGTACTCGAAC GCCTCACCCGCCTCCGAACTCTTGAAACCCCGGCTTCTGCTCTAACTCTTACCATCTGGT TTCTTGACCCCCCTTTTCTATGACTCTTATCCACCCCCTACCTTTCCACGCCCCCGCCTC ACAACCCTCTCTCTTTCTGACCCCCCTTTTTCTTTCCTCACTCTCTCCTCCCCTCCCTCC CCCCTCGTACCTCTCTATCCCTGTCTTCCACCCTCCTCGCCTCCTCTGACCCATCCTCCT TTCTTTCATTATTAAGTCCTCTCTCACTCTATTTTCGTTAGCCTTCATTTCACTATCTCT ACGGCCCTCCTCCTCTCTAATTATTCCAACACCTTCCTCATCCTTTTCTATTTCTAAAAC CCGTCCTTCAATCACAATTCTCATCCCTCTTCTATATCCTTATCCCTCCGTTTCCCCCAC TCTTTACTCTCTCTTATCT |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_020165 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020165.2, NP_064550.2 |
| RefSeq Size | 3053 bp |
| RefSeq ORF | 1488 bp |
| Locus ID | 56852 |
| UniProt ID | Q9NS91 |
| Domains | RING, SAP, ZnF_Rad18 |
| Protein Families | Druggable Genome |
| Gene Summary | The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq, Jul 2008] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201700 | RAD18 (Myc-DDK-tagged)-Human RAD18 homolog (S. cerevisiae) (RAD18) |
CNY 3,656.00 |
|
| RC201700L1 | Lenti ORF clone of Human RAD18 homolog (S. cerevisiae) (RAD18), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC201700L2 | Lenti ORF clone of Human RAD18 homolog (S. cerevisiae) (RAD18), mGFP tagged |
CNY 6,056.00 |
|
| RC201700L3 | Lenti ORF clone of Human RAD18 homolog (S. cerevisiae) (RAD18), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC201700L4 | Lenti ORF clone of Human RAD18 homolog (S. cerevisiae) (RAD18), mGFP tagged |
CNY 6,056.00 |
|
| RG201700 | RAD18 (tGFP-tagged) - Human RAD18 homolog (S. cerevisiae) (RAD18) |
CNY 5,256.00 |
|
| SC323786 | RAD18 (untagged)-Human RAD18 homolog (S. cerevisiae) (RAD18) |
CNY 3,656.00 |
