RAD18 (NM_020165) Human Untagged Clone

CAT#: SC108413

RAD18 (untagged)-Human RAD18 homolog (S. cerevisiae) (RAD18)



  "NM_020165" in other vectors (7)

CNY 3,656.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit polyclonal antibody to RAD18 (RAD18 homolog (S. cerevisiae))
    • 100 ul

CNY 6,281.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms RNF73
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC108413 sequence for NM_020165 edited (data generated by NextGen Sequencing)
ATGGACTCCCTGGCCGAGTCTCGGTGGCCTCCGGGCCTGGCAGTCATGAAGACAATAGAT
GATTTGCTGCGGTGTGGAATTTGCTTCGAGTATTTCAACATTGCAATGATAATACCTCAG
TGTTCACATAACTACTGCTCTCTCTGTATAAGAAAATTTCTGTCCTATAAAACTCAGTGT
CCAACTTGCTGTGTGACTGTCACAGAGCCGGATCTGAAAAATAACCGCATATTAGATGAA
CTGGTAAAAAGCTTGAATTTTGCACGGAATCATCTGCTGCAGTTTGCTTTAGAGTCACCA
GCCAAATCTCCTGCTTCTTCCTCTTCAAAGAATCTTGCTGTCAAAGTATATACTCCTGTA
GCCTCCAGACAGTCTTTAAAGCAGGGGAGCAGGTTAATGGATAATTTCTTGATCAGAGAA
ATGAGTGGTTCTACATCAGAGTTGTTGATAAAAGAAAATAAAAGCAAATTCAGCCCTCAA
AAAGAGGCGAGCCCTGCTGCAAAGACCAAAGAGACACGTTCTGTAGAAGAGATCGCTCCA
GATCCCTCAGAGGCTAAGCGTCCTGAGCCACCCTCGACATCCACTTTGAAACAAGTTACT
AAAGTGGATTGTCCTGTTTGCGGGGTTAACATTCCAGAAAGTCACATTAATAAGCATTTA
GACAGCTGTTTATCACGCGAAGAGAAGAAGGAAAGCCTCAGAAGTTCTGTTCACAAAAGG
AAGCCGCTGCCCAAAACTGTATATAATTTGCTCTCTGATCGTGATTTAAAGAAAAAGCTA
AAAGAGCATGGATTATCTATTCAAGGAAATAAACAACAGCTCATTAAAAGGCACCAAGAA
TTTGTACACATGTACAATGCCCAATGCGATGCTTTGCATCCTAAATCAGCTGCTGAAATA
GTTCGAGAAATCGAAAATATAGAGAAGACTAGGATGCGTCTTGAAGCTAGTAAACTCAAT
GAAAGTGTAATGGTTTTTACAAAGGACCAAACAGAAAAGGAAATAGATGAAATCCACAGT
AAATATCGTAAAAAACATAAGAGTGAATTTCAGCTTCTGGTGGATCAGGCTAGAAAAGGA
TACAAGAAAATTGCTGGAATGTCACAAAAAACAGTAACAATAACAAAAGAAGATGAATCT
ACAGAAAAGCTATCTTCTGTATGCATGGGACAGGAAGATAATATGACCTCAGTAACAAAC
CACTTTTCTCAATCAAAGCTGGACTCCCCAGAGGAATTGGAACCTGACAGAGAAGAGGAT
TCTTCTAGCTGTATTGATATTCAAGAAGTTCTTTCTTCATCAGAATCAGATTCATGCAAT
AGTTCCAGTTCAGACATCATAAGAGATCTTTTAGAAGAAGAGGAAGCCTGGGAAGCATCA
CATAAAAACGATCTTCAAGACACAGAAATAAGTCCAAGACAGAATCGCCGCACAAGAGCC
GCTGAAAGTGCTGAGATTGAACCAAGAAACAAGCGTAATAGGAATTAA

Clone variation with respect to NM_020165.3
>OriGene 5' read for NM_020165 unedited
GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATTCGG
CACGAGGGACCCCCAGCATCCTCGAGAGCGACCATGGACTCCCTGGCCGAGTCTCGGTGG
CCTCCGGGCCTGGCAGTCATGAAGACAATAGATGATTTGCTGCGGTGTGGAATTTGCTTC
GAGTATTTCAACATTGCAATGATAATACCTCAGTGTTCACATAACTACTGCTCTCTCTGT
ATAAGAAAATTTCTGTCCTATAAAACTCAGTGTCCAACTTGCTGTGTGACTGTCACAGAG
CCGGATCTGAAAAATAACCGCATATTAGATGAACTGGTAAAAAGCTTGAATTTTGCACGG
AATCATCTGCTGCAGTTTGCTTTAGAGTCACCAGCCAAATCTCCTGCTTCTTCCTCTTCA
AAGAATCTTGCTGTCAAAGTATATACTCCTGTAGCCTCCAGACAGTCTTTAAAGCAGGGG
AGCAGGTTAATGGATAATTTCTTGATCAGAAAATGAGTGGTTCTACTCAGAGTTGTTGAT
AAAGAAATAAAAGCAATTCACCCTCAAAAGAGCGAGCCCTGCTGAAAGACCAAGAGACAC
GTTCTGAAAGAGATCGCTCAATCCTCAAGGCTAACGTCTGAGCCACCTCGACATCCTTGA
ACAAGTACTAAGTGGATNNGTCTGTTGCGGGNTACATTCAAAAAGCCATTTATACATTAC
CAGCTGNTATCACCGAAAGAAGAAGCCTAAAANTTTGTACAAAGAAGCCCTCCAAACTGT
TTAATNGCTCTTGGACGGTTAAGAAACTAAAACTGGGTTTTCTTCAGAATACACGCTCTA
AAGCCCAAATTTNCCCGCCAGGCAGGGAGGTTTCTC
>OriGene 3' read for NM_020165 unedited
TCTCGCTGTACGACCCACTCTTAGCACTAGATAAGTAGTCATGNAAACAAACAGTTTTCA
TTTTGACATAATTTCTGACAACTCAGTGACATAAATATATCCATGTGAGCTCCTATTTTG
TTTGACATGCTCACTTACCTCACATCAGTTCTGCCGATAGATTTGGTAAGTGGTGCTTTT
AGTTTCCTTAATGCACCCCTCAGGATTTCATGCAAATGAAATTATTATGACAAATTTTTC
TATAGCCATTTCTAACACAGGGAACTCATAAAGAAATCAAATGTCTGCACTTCACTGTGA
AAACACTAAACTCTCAGCCATAATGAGACTCCACACATTATCAGAAACCCCAAGGCATAC
CAAAAAGACAAGGTCAACGGAGACAGCCTCTGGAAGGGGAGGTCATACCTGCCGCAACCT
GGCCAACCATAAATCTCTAGTTTTAACATTGCCGCATTTACTATCATCTCATTTGAAAGC
TGATGTGCTTGGGCTGTTTGATAACCCCAGGCGTGGACTCGTGCTATGCTGTACTCGAAC
GCCTCACCCGCCTCCGAACTCTTGAAACCCCGGCTTCTGCTCTAACTCTTACCATCTGGT
TTCTTGACCCCCCTTTTCTATGACTCTTATCCACCCCCTACCTTTCCACGCCCCCGCCTC
ACAACCCTCTCTCTTTCTGACCCCCCTTTTTCTTTCCTCACTCTCTCCTCCCCTCCCTCC
CCCCTCGTACCTCTCTATCCCTGTCTTCCACCCTCCTCGCCTCCTCTGACCCATCCTCCT
TTCTTTCATTATTAAGTCCTCTCTCACTCTATTTTCGTTAGCCTTCATTTCACTATCTCT
ACGGCCCTCCTCCTCTCTAATTATTCCAACACCTTCCTCATCCTTTTCTATTTCTAAAAC
CCGTCCTTCAATCACAATTCTCATCCCTCTTCTATATCCTTATCCCTCCGTTTCCCCCAC
TCTTTACTCTCTCTTATCT
Restriction Sites ECoRI-NOT     
ACCN NM_020165
Insert Size 3000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_020165.2, NP_064550.2
RefSeq Size 3053 bp
RefSeq ORF 1488 bp
Locus ID 56852
UniProt ID Q9NS91
Domains RING, SAP, ZnF_Rad18
Protein Families Druggable Genome
Gene Summary The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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