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SURF5 (MED22) (NM_181491) Human Untagged Clone
CAT＃: SC324098
MED22 (untagged)-Human mediator complex subunit 22 (MED22), transcript variant c
CNY 1,200.00
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MED24; SRB6; surf-5; SURF5 |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_181491.1
CGGGGCCGAGAGGCTGCGGCGGCTGACTAGGTTTTCGGACCGCGGCGCGGCGGGGAAGAG
AGACTGCGGCGGGAGGGCCAGGCCGTGGGAGAGACGCGGGAAAGTGCGGGTCGCAGAGGC ACTTGACCACCCCGCCCTAGGCCGACCCGGCGGACGCGGCGTCTGGTGTGCGGGCGCTGG GGCGGGTCCCAGGTCTCCCCGCTGCGCTGCTTGAGGCTCGGCCATGGCCCAGCAGAGAGC CCTGCCCCAGAGCAAGGAGACGCTGCTGCAGTCCTACAACAAGCGGCTGAAGGACGACAT TAAGTCCATCATGGACAACTTCACCGAGATCATCAAGACCGCCAAGATTGAGGACGAGAC GCAGGTGTCACGGGCCACTCAGGGTGAACAGGACAATTACGAGATGCATGTGCGAGCCGC CAACATCGTCCGAGCCGGCGAGTCCCTGATGAAGCTGGTGTCCGACCTCAAGCAGTTCCT GATCCTCAATGACTTCCCCTCCGTGAACGAGGCCATTGACCAGCGCAACCAGCAGCTGCG CACACTGCAGGAGGAGTGCGACCGGAAGCTCATCACGCTGCGAGACGAGATCTCCATTGA CCTCTACGAGCTGGAGGAGGAGTATTACTCGTCCAGGTATAAATAGCGCTGGACTCCCCA TGCAGAGCGGGAGCCTGCCTACCTGGGCCTGGCCAGCAGGCAGGGCTGCCTTCTGCTTTT TCAAATTCTTGCTGGTCTTAGCAGTGGAGCCATGCCTGGGTTTCAGAGCAGAGCTCCTGG CCAGAGCGTTTGACCGACAGACAATTCACATCCATATGCCAGGGCCCTGGGCCTTTCCCA CAGTGCAATGTGATGAAAACCACAGGACTCACGCCAGTCGGATAGGCCGAGTCTGGAGAA GGGAGGCGCCTGGCTGTATCCCCCGCAGGCCCTCTTCCGAGAGCCTTCCTCCTCGGGCAG TGCGTTCTGGGGCTGTGCTGCTCCTGTTACCTTCTGAATCCATATGTAGAGATTTCAGCC AAGGCTGGGCCAGCCTTTTTTGGGCAGTCAGGTCCACACCTATGTCCAGGGCACCAGGGA TGCAATTCCATGTGGATGTCACCAAACCCCAGTGTGGAGGCAGGGACAGTCATGGGAATG TGGGGGATGAAGCCCAGGCAGGGAATGGCCTTGAAAGCCATTGGAGCTCCAATTCGTGAC CCACTCAGCCTTATCCACGGAGCTGGAGCCAACCTACGTGCCAGGCCCCGTGCTGGGTCC CAGGGATGCAGAAGGGTCAAAACCCATCATCCTGACCCTTGTGGGGCTCCGTAAGAAGCT GAAACCTTCGACCGTTTGAGCTGGAGGGGCCCTGAGAAATCAGAGTCTACGTATCATTTA CTTAGGGGGAAACTTAGGCTGGAGACAGGGAGGCCTTCCACTCTGCCCCATTAGCTTAGA AAATCAAGATTCAGTCCAGCAGATGCAGAGTCCATGTCCATCTTGTGCCTTCTCCTGGAC AAACCTTTCCTTCCTGGTGGTGGATTTAAAATACTCCTTTCTGCCCATTGGCCATGCTGG GAGCCACAGATATCCAGAGCCAGCATGACCTGGGGCTTGGTTTCCCTGCCCTGGGCTCAG TGGCACTGCTGAGCTGCAGCAGTCCTAGAGTTTTCCAGGGGGTTCTGAGGGAATCTTTGG TCCCCAGTACTCATTAACTCAGCAGACATGAGGCAGCATTTCCTCCACACTAGGGTGGCT GAGAGGGGTCCTAGGGTGTTTCAGACCCTTCTGGGCATCTCCTTCCACAGCTGTTCAGTT TGTCGGTCTCTTTGAGGCAGCCACCGTCCCTGAGGGCCCCTGCACAGAGCAGCTGTGGGC CTGTAATTCAGCCTGCCTGCCTTGCCTTGGGGCAGGGAGAGAGGGAACCTGCTCACGGCC CTGCAGCAGAGCAGGGCGCAAACCCAGGACATCTGTGCCAGGCTTCCCATGCCCTCCCCC AACAGTCCCTCAGCTTCACCCAGCGGGGCTTCCAGGCCAGCCTGTGTCCCCTCCCGCAGG CCTCCTGTCCACACCAGCGCCCCCTGGGGGGCCTCACACAGCCCCTGTGGCAGAAGCAGT TGCCCTCCTCTGTACATTGCCTTTAAGCGACCAGGTCCTGGCCGAGTTTCCTCTGCCCCT TCTTGCTGGTCCCCCAAAGGGCGCTCCGCTCCCTGCCCTGCCCTGCCCTGTTCCGCATGA GCTGCGCCTCTGTGCTCGCCTGCCCCCTCTCTGCTTGTTAGTTGCTCTTTCTGGCTCTGC CTCTCCTTTGCTTTCCTCGGGATGCCACTCTGTGCCCAGGACGGTTCTGAGACTGAACAC TGAGGGCAGGAGCAAGGGAGGAAGCCAGGGGCGAGGCAGGCCGCGGGAAAGCCAGGGCCC CTGCCTGCAGGTTAGAAAGAGGCGAGCGTGGATTGTCACAGCTGCGGGCATGGGAAGGGC TAGCTGAGCTCTTCACCTGCATCCTGGCTGCCGTGAGGATTCCCCGTGTTAGAGGTGGGG ACGCCTGCTGGAGGCCGCCTGGCTGATGTAGGGCTATCGGGAAGTGCCAGGGCCTGTGTT CCCAACTGTCGCCCCCTTCAGGCTAAGTCTCAGGCAGGGACAGACCCAGAAAGAACACAG TCTGCCCTCAGAGAGCTCTTTGCAGTGTAGTGACACTGGGGTTTCTGCAGTCAGGGAGGA GGGAGGGTGGCCAGGCTGACAGCTTTTTGCAAGAGGAGGGGGACCAGCACCAGCTGGGAG GCATAGGCTAGGACAGGCCCACGTGGAGGCTGGGCAGGAAGGGCCTGCTGAGGTCACACA GCTGTTGGTGGTTGGGCCAGGGCGGCTTCCTCCTTTCAGAATGCTAGGGTGGCTCTCACC ACTGGCCGCCTCTCCTTGCCAGACCTGCCAACTCAGGGGACAGATGGAGCAGGAGTGGAG AAAGGGAAAGGCAGGTCTGGGGTGTGGTCGTGTTTTCTTAACTCTGCTTCTGTCTTGCTC TCCCCTCCCCTGGCTTTCCTCTCTGCCTGCTCCTGTCTCTCCCTGGGGTTTCTGGTGGTG GAAAAGCTCAAGCCTTTGCGAAGCTAATGACCTGCCTCTGTGCGAAGCTTACGGGAGGCT GGACCTCGACACAGACTCTGCTGATGGCCTCTCGGCCCCTCTGCTGGCGTCCCCGGAGCC CAGTGCTGGCCCCCTACAGGTGGCAGCCCCTGCCCACTCCCATGCTGGTGGCCCTGGCCC CACTGAGCACGCCTGAGCCTCCGGGGCCACGCTTCGTTCTCAGGAACAAAACCTGAGGCA GCCCTTTGGATGCCCTCACAGCCTTGCTTCTCTCAGCCTAGGTTCCCATTTGGGGACTTC AGGACCCCAGAGCCACTAGGACTTCCTTGGGAAGCCCGTTAGCCCAGGGTGGGTCCCGCC AGGACAGTAGGGAAACAGTTGTTTCCCTAGCCATTTCCGAATAGCCCATCATTCCGAGTC ATCATCTCTGTTTGCTGCCTTCCTGGCCAGCCAGGTGGAAGAAAGTTTCCAAGCTAGGTC TGGCCCGTTGGGGATCTCAGCAGTGGAGCAGGAGGGTGCCTGATTTCGGGGAGTCCTGAC CCGAGCCTGTTGTCAGAGTTGGGAGGGGCTCTGAGCAGTGTTGGGCAGGCCGGGTCTCCC ATCCCGAGGCCAGCGTTCCTGTGCAGAGCCCCATCCACTGGTTCTTGCCCTGAGCCACAT ATGTCTGTGCCATGGGCTGAGTGCCACGACAGGCCCGTGTGACAGCTGCTGCCCACGCAT GTGGAAGCTAGGTGGGACTCATTCCTAATTCTGCCGTTGTAATGAGACTTGATTAAAACA CCGCCACTTTTTTGCAAAAAAAAAAAAAAAA |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_181491 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_181491.1, NP_852468.1 |
| RefSeq Size | 3887 bp |
| RefSeq ORF | 423 bp |
| Locus ID | 6837 |
| UniProt ID | Q15528 |
| Gene Summary | This gene encodes a protein component of the mediator complex, which functions in the regulation of transcription by bridging interactions between gene-specific regulatory factors, RNA polymerase II, and general transcription factors. Alternatively spliced transcript variants encoding different isoforms have been observed. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (c) differs in the 3' UTR and its transcription extends past a splice site used in variant b, resulting in a distinct 3' coding region. The encoded isoform (c) is shorter and has a distinct C-terminus, compared to isoform b. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208015 | MED22 (Myc-DDK-tagged)-Human mediator complex subunit 22 (MED22), transcript variant c |
CNY 1,200.00 |
|
| RC208015L3 | Lenti ORF clone of Human mediator complex subunit 22 (MED22), transcript variant c, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208015L4 | Lenti ORF clone of Human mediator complex subunit 22 (MED22), transcript variant c, mGFP tagged |
CNY 5,890.00 |
|
| RG208015 | MED22 (tGFP-tagged) - Human mediator complex subunit 22 (MED22), transcript variant c |
CNY 2,800.00 |
