United States
Germany
Japan
United Kingdom
China
SAMD8 (NM_001174156) Human Untagged Clone
CAT＃: SC328676
SAMD8 (untagged)-Human sterile alpha motif domain containing 8 (SAMD8) transcript variant 1
CNY 6,650.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HEL-177; SMSr |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC328676 representing NM_001174156.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCAGGTCCTAATCAACTCTGCATTCGCCGCTGGACTACCAAGCATGTAGCTGTGTGGCTGAAGGAT GAAGGCTTTTTTGAATATGTGGACATTTTATGCAATAAGCACCGACTTGATGGAATCACATTGCTAACA TTGACTGAATATGATCTCCGGTCTCCTCCTCTGGAAATCAAAGTCTTAGGGGACATTAAAAGGTTAATG CTCTCAGTCCGAAAATTGCAGAAAATACATATTGATGTTTTAGAAGAGATGGGCTACAACAGTGACAGT CCCATGGGTTCCATGACCCCTTTCATCAGTGCTCTTCAGAGTACAGACTGGCTCTGTAATGGGGAGCTT TCCCATGACTGTGACGGACCCATAACTGACTTGAATTCTGATCAGTACCAGTACATGAATGGTAAAAAC AAACATTCTGTTCGAAGATTGGACCCAGAATACTGGAAGACTATACTGAGTTGTATATATGTTTTTATA GTATTTGGATTTACATCTTTCATTATGGTTATAGTCCATGAGCGAGTGCCTGACATGCAGACCTATCCA CCACTCCCAGATATATTCTTAGACAGCGTTCCTAGAATCCCATGGGCCTTTGCCATGACGGAAGTATGT GGCATGATTCTGTGCTATATTTGGCTCCTGGTTCTTCTTCTTCACAAGCACAGGTCAATACTTCTGCGA AGGCTCTGTAGTCTGATGGGAACTGTATTCTTGCTTCGCTGCTTTACCATGTTTGTGACCTCCCTCTCC GTGCCAGGACAACACCTGCAGTGTACTGGAAAGATATATGGCAGTGTATGGGAGAAATTACATCGAGCC TTTGCCATTTGGAGTGGCTTTGGTATGACCCTGACTGGCGTTCACACATGTGGAGATTACATGTTTAGT GGCCACACAGTCGTCCTAACTATGCTGAATTTCTTTGTCACCGAATATACACCAAGAAGCTGGAATTTC TTGCACACTTTATCCTGGGTTCTCAACCTCTTTGGAATCTTCTTCATCTTGGCTGCCCATGAACATTAT TCTATTGATGTGTTTATTGCTTTTTATATAACAACAAGACTCTTTTTGTACTACCATACTCTGGCCAAT ACCAGAGCATATCAGCAGAGTAGGAGAGCAAGGATTTGGTTTCCCATGTTCTCTTTTTTTGAATGCAAT GTTAATGGCACAGTACCTAATGAATATTGTTGGCCATTTTCAAAACCAGCAATAATGAAAAGACTAATT GGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001174156 |
| Insert Size | 1248 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001174156.1 |
| RefSeq Size | 6782 bp |
| RefSeq ORF | 1248 bp |
| Locus ID | 142891 |
| UniProt ID | Q96LT4 |
| Protein Families | Transmembrane |
| MW | 48.3 kDa |
| Gene Summary | Sphingomyelin synthases synthesize sphingolipids through transfer of a phosphatidyl head group on to the primary hydroxyl of ceramide. SAMD8 is an endoplasmic reticulum (ER) transferase that has no sphingomyelin synthase activity but can convert phosphatidylethanolamine (PE) and ceramide to ceramide phosphoethanolamine (CPE) albeit with low product yield. Appears to operate as a ceramide sensor to control ceramide homeostasis in the endoplasmic reticulum rather than a converter of ceramides. Seems to be critical for the integrity of the early secretory pathway.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230038 | SAMD8 (Myc-DDK-tagged)-Human sterile alpha motif domain containing 8 (SAMD8), transcript variant 1 |
CNY 3,656.00 |
|
| RC230038L3 | Lenti ORF clone of Human sterile alpha motif domain containing 8 (SAMD8), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC230038L4 | Lenti ORF clone of Human sterile alpha motif domain containing 8 (SAMD8), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG230038 | SAMD8 (tGFP-tagged) - Human sterile alpha motif domain containing 8 (SAMD8), transcript variant 1 |
CNY 4,370.00 |
