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HHAT (NM_001170587) Human Untagged Clone
CAT＃: SC328809
HHAT (untagged)-Human hedgehog acyltransferase (HHAT) transcript variant 5
CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MART2; SKI1; Skn |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_001170587, the custom clone sequence may differ by one or more nucleotides
ATGTCGCTGGGACTCGGAAGTGCCGAAAGAGGGGTGTTGGGAACTCGCGGCGCGCGTGAA CGTTGCCGTCGCCGCCGCCCGGGACAGCCCGGAGAACACGAAGAGGAGCTGGACCAGGAA TTTGAGCTGGAGACTGACACTTTATTTGGAGGATTAAAGAAGGATGCGACCGACTTTGAG TGGAGCTTCTGGATGGAATGGGGGAAGCAGTGGCTGGTGTGGCTTCTCCTTGGCCACATG GTAGTGTCTCAAATGGCCACACTGCTGGCAAGAAAGCACAGACCCTGGATTCTCATGCTC TATGGGATGTGGGCCTGCTGGTGTGTGCTGGGGACCCCTGGTGTGGCTATGGTTTTGCTC CATACCACCATCTCTTTCTGCGTGGCCCAGTTCCGGTCTCAGCTCCTGACGTGGCTCTGT TCTCTCCTCCTCCTCTCCACACTGAGGCTGCAGGGTGTGGAAGAAGTTAAGAGAAGGTGG TACAAGACAGAAAACGAGTACTACCTGCTGCAGTTCACGCTGACCGTTCGCTGCCTGTAC TACACCAGCTTCAGCCTGGAGCTCTGCTGGCAGCAGCTGCCTGCTGCATCGACCTCCTAC TCCTTTCCCTGGATGCTGGCCTATGTCTTTTATTATCCAGTCTTACACAATGGGCCCATC CTCAGCTTCTCGGAGTTCATCAAACAGATGCAGCAGCAGGAGCATGACTCCCTGAAGGCC AGCCTGTGTGTCCTGGCCCTGGGGCTGGGCCGCCTTCTTTGCTGGTGGTGGCTGGCCGAG CTGATGGCTCACCTGATGTACATGCATGCCATCTACAGCAGCATCCCCCTCCTGGAGACT GTCTCTTGTTGGACCTTAGGAGGACTGGCGTTAGCCCAGGTGCTCTTTTTCTACGTGAAG TACTTGGTGCTCTTTGGCGTGCCTGCTCTGCTCATGCGCCTGGATGGACTCACTCCACCC GCCCTCCCCCGCTGCGTGAGCACCATGTTCAGTTTCACCGGGATGTGGAGGTATTTTGAT GTTGGACTGCATAATTTCTTAATCAGGTATGTGTACATTCCAGTGGGCGGGTCCCAGCAT GGCCTGCTGGGGACACTGTTTTCCACGGCGATGACATTTGCATTTGTGAGCTACTGGCAT GGCGGCTACGACTACCTCTGGTGCTGGGCAGCGCTCAACTGGCTGGGAGTCACTGTGGAG AATGGAGTCCGGAGGCTGGTGGAGACTCCCTGCATCCAGGACAGTCTGGCCCGATACTTC TCCCCACAAGCTCGCCGTCGATTCCACGCTGCCCTTGCTTCTTGTTCCACCTCGATGCTG ATCCTGTCCAACCTGGTATTTCTTGGGGGCAATGAGGTTGGGAAAACCTACTGGAATAGG ATCTTCATACAAGGCTGGCCTTGGGTGACCCTCTCTGTCCTGGGATTCCTGTACTGCTAC TCCCACGTGGGCATTGCCTGGGCCCAGACCTACGCCACGGACTAA |
| Restriction Sites | Please inquire |
| ACCN | NM_001170587 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001170587.1, NP_001164058.1 |
| RefSeq Size | 3507 bp |
| RefSeq ORF | 1485 bp |
| Locus ID | 55733 |
| UniProt ID | Q5VTY9 |
| Protein Families | Transmembrane |
| Gene Summary | 'Skinny hedgehog' (SKI1) encodes an enzyme that acts within the secretory pathway to catalyze amino-terminal palmitoylation of 'hedgehog' (see MIM 600725).[supplied by OMIM, Jul 2002] Transcript Variant: This variant (5) differs in the 5' UTR and 5' coding region and initiates translation at an upstream start codon, compared to variant 1. The encoded isoform (4) has a longer N-terminus, compared to isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230171 | HHAT (Myc-DDK-tagged)-Human hedgehog acyltransferase (HHAT), transcript variant 5 |
CNY 3,656.00 |
|
| RC230171L3 | Lenti-ORF clone of HHAT (Myc-DDK-tagged)-Human hedgehog acyltransferase (HHAT), transcript variant 5 |
CNY 6,180.00 |
|
| RC230171L4 | Lenti-ORF clone of HHAT (mGFP-tagged)-Human hedgehog acyltransferase (HHAT), transcript variant 5 |
CNY 6,180.00 |
|
| RG230171 | HHAT (tGFP-tagged) - Human hedgehog acyltransferase (HHAT), transcript variant 5 |
CNY 4,750.00 |
