DMT1 (SLC11A2) (NM_001174130) Human Untagged Clone

CAT#: SC328906

SLC11A2 (untagged)-Human solute carrier family 11 (proton-coupled divalent metal ion transporters) member 2 (SLC11A2) transcript variant 7



  "NM_001174130" in other vectors (4)

CNY 9,030.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit polyclonal SLC11A2 Antibody (Center)
    • 400 ul

CNY 6,314.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms AHMIO1; DCT1; DMT1; NRAMP2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC328906 representing NM_001174130.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGTCCACGGTCGATTATTTGAACAGTGTTTCTGGAGATCATGGGGAGTCTGCCAGTCTTGGTAACATC
AACCCTGCCTATAGTAATCCCTCTCTTTCACAGTCCCCTGGGGACTCAGAGGAGTACTTCGCCACTTAC
TTTAATGAGAAGATCTCCATTCCTGAGGAGGAGTACTCTTGTTTTAGCTTTCGTAAACTCTGGGCTTTC
ACCGGACCAGGTTTTCTTATGAGCATTGCCTACCTGGATCCAGGAAATATTGAATCCGATTTGCAGTCT
GGAGCAGTGGCTGGATTTAAGTTGCTCTGGATCCTTCTGTTGGCCACCCTTGTGGGGCTGCTGCTCCAG
CGGCTTGCAGCTAGACTGGGAGTGGTTACTGGGCTGCATCTTGCTGAAGTATGTCACCGTCAGTATCCC
AAGGTCCCACGAGTCATCCTGTGGCTGATGGTGGAGTTGGCTATCATCGGCTCAGACATGCAAGAAGTC
ATTGGCTCAGCCATTGCTATCAATCTTCTGTCTGTAGGAAGAATTCCTCTGTGGGGTGGCGTTCTCATC
ACCATTGCAGATACTTTTGTATTTCTCTTCTTGGACAAATATGGCTTGCGGAAGCTAGAAGCATTTTTT
GGCTTTCTCATCACTATTATGGCCCTCACATTTGGATATGAGTATGTTACAGTGAAACCCAGCCAGAGC
CAGGTACTCAAGGGCATGTTCGTACCATCCTGTTCAGGCTGTCGCACTCCACAGATTGAACAGGCTGTG
GGCATCGTGGGAGCTGTCATCATGCCACACAACATGTACCTGCATTCTGCCTTAGTCAAGTCTAGACAG
GTAAACCGGAACAATAAGCAGGAAGTTCGAGAAGCCAATAAGTACTTTTTCATTGAATCCTGCATTGCA
CTCTTTGTTTCCTTCATCATCAATGTCTTTGTTGTCTCAGTCTTTGCTGAAGCATTTTTTGGGAAAACC
AACGAGCAGGTGGTTGAAGTCTGTACAAATACCAGCAGTCCTCATGCTGGCCTCTTTCCTAAAGATAAC
TCGACACTGGCTGTGGACATCTACAAAGGGGGTGTTGTGCTGGGATGTTACTTTGGGCCTGCTGCACTC
TACATTTGGGCAGTGGGGATCCTGGCTGCAGGACAGAGCTCCACCATGACAGGAACCTATTCTGGCCAG
TTTGTCATGGAGGGATTCCTGAACCTAAAGTGGTCACGCTTTGCCCGAGTGGTTCTGACTCGCTCTATT
GCCATCATCCCCACTCTGCTTGTTGCTGTCTTCCAAGATGTAGAGCATCTAACAGGGATGAATGACTTT
CTGAATGTTCTACAGAGCTTACAGCTTCCCTTTGCTCTCATACCCATCCTCACATTTACGAGCTTGCGG
CCAGTAATGAGTGACTTTGCCAATGGACTAGGCTGGCGGATTGCAGGAGGAATCTTGGTCCTTATCATC
TGTTCCATCAATATGTACTTTGTAGTGGTTTATGTCCGGGACCTAGGGCATGTGGCATTATATGTGGTG
GCTGCTGTGGTCAGCGTGGCTTATCTGGGCTTTGTGTTCTACTTGGGTTGGCAATGTTTGATTGCACTG
GGCATGTCCTTCCTGGACTGTGGGCATACGGTAAGCATCTCTAAAGGCCTGCTGACAGAAGAAGCCACC
CGTGGCTACGTTAAATAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_001174130
Insert Size 1674 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001174130.1
RefSeq Size 4139 bp
RefSeq ORF 1674 bp
Locus ID 4891
UniProt ID P49281
Protein Families Transmembrane
Protein Pathways Lysosome
MW 61 kDa
Gene Summary This gene encodes a member of the solute carrier family 11 protein family. The product of this gene transports divalent metals and is involved in iron absorption. Mutations in this gene are associated with hypochromic microcytic anemia with iron overload. A related solute carrier family 11 protein gene is located on chromosome 2. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Apr 2010]
Transcript Variant: This variant (7) differs in the 5' UTR and 5' coding region compared to variant 1. The resulting protein (isoform 4) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...