Chromosome X open reading frame 6 (MAMLD1) (NM_001177466) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CG1; CXorf6; F18; HYSP2
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001177466, the custom clone sequence may differ by one or more nucleotides ATGGATGACTGGAAAAGTCGGCTTGTAATCAAGAGCATGCTTCCCCATTTCGCCATGGTG GGAAATCGTCAGGAGCCCAGAAAGCTCCAGGAATCGGGAACTGTTAAGAGGAGACAAGAA GAAGACCACTTCCAGTTTCCAGACATGGCTGATGGGGGCTACCCTAATAAAATTAAGAGG CCTTGCCTTGAAGATGTCACCCTTGCAATGGGCCCAGGTGCTCATCCTAGTACTGCTTGT GCAGAACTGCAGGTCCCTCCATTGACAATAAATCCTAGCCCTGCGGCTATGGGAGTGGCT GGCCAGTCATTACTGCTGGAGAATAACCCTATGAATGGCAACATCATGGGCTCACCATTT GTAGTACCACAGACTACAGAAGTGGGACTGAAAGGGCCCACTGTTCCTTACTATGAGAAA ATCAACAGCGTGCCGGCTGTAGACCAGGAGCTTCAAGAGCTGCTAGAGGAGCTCACCAAA ATTCAAGACCCTTCTCCAAATGAGCTAGATCTTGAGAAGATACTGGGGACGAAGCCAGAA GAGCCACTGGTTTTAGATCATCCCCAGGCAACCCTAAGCACAACTCCCAAGCCTTCGGTT CAGATGTCACACTTGGAGAGCCTGGCTTCCAGCAAGGAGTTTGCTTCTAGTTGCAGCCAA GTTACTGGCATGTCACTTCAGATCCCATCCTCCTCCACAGGGATCAGCTATTCGATTCCT TCCACCAGTAAGCAGATAGTGTCACCGAGTTCTTCAATGGCACAGTCCAAGAGCCAGGTC CAGGCCATGCTCCCTGTCGCTCTGCCCCCCTTACCAGTGCCTCAGTGGCATCACGCCCAC CAGCTGAAGGCGTTGGCAGCCAGCAAGCAGGGGTCTGCTACAAAGCAGCAAGGGCCCACC CCCAGTTGGTCTGGTCTGCCTCCTCCAGGACTCTCTCCACCTTACCGCCCAGTGCCATCA CCACACCCACCACCGCTGCCACTGCCACCACCACCACCCCCATTCAGCCCCCAGAGCCTC ATGGTGTCCTGCATGTCGTCCAATACCTTGTCGGGTAGCACTCTCCGAGGCTCTCCCAAT GCCTTACTGTCAAGCATGACGTCCAGCAGCAATGCTGCCCTGGGGCCCGCCATGCCCTAT GCTCCTGAGAAGCTCCCCAGCCCTGCTCTCACTCAACAGCCGCAGTTCGGCCCTCAGAGC TCCATTCTTGCCAACCTCATGTCCTCTACCATCAAAACCCCTCAAGGACACCTGATGTCT GCTCTGCCTGCCAGCAACCCTGGGCCGTCCCCACCCTATCGCCCAGAGAAGCTCTCTAGC CCAGGCTTGCCACAGCAGTCCTTCACCCCACAGTGTTCCCTGATCCGAAGCCTCACTCCC ACCAGTAATCTTCTAAGCCAGCAACAGCAGCAGCAGCAGCAGCAGCAGCAAGCAAATGTG ATCTTTAAGCCCATAAGCAGCAACTCATCCAAAACCCTGAGCATGATCATGCAGCAGGGG ATGGCAAGCTCCAGCCCAGGAGCCACGGAGCCATTTACTTTTGGCAACACCAAGCCCTTG TCCCATTTTGTTTCTGAGCCGGGTCCCCAGAAGATGCCCTCCATGCCTACCACCTCTAGG CAGCCTTCCCTGCTCCACTACCTGCAGCAGCCGACACCAACGCAGGCCTCCTCAGCCACT GCCTCCTCCACGGCCACTGCCACCTTGCAGCTGCAGCAGCAGCAGCAGCAACAGCAGCAG CAGCCTGACCATTCTTCATTCCTTCTGCAGCAGATGATGCAGCAACCCCAGCGTTTTCAG CGATCAGTGGCCTCAGATTCCATGCCTGCTCTGCCCAGACAGGGCTGTTGCCATCTGTTT GCATGGACTTCTGCAGCTAGCTCGGTGAAGCCCCAGCATCAACACGGGAACTCTTTCACT AGCAGGCAAGATCCTCAGCCTGGAGACGTGTCACCGTCTAACATTACTCATGTAGACAAA GCCTGCAAGCTTGGGGAAGCCAGGCACCCCCAGGTCAGCCTCGGGCGACAGCCCCCGTCC TGCCAGGCCCTGGGGAGTGAGTCCTTCCTGCCCGGCAGCTCCTTTGCTCATGAGCTGGCC CGAGTCACCTCCTCGTACAGCACCTCAGAGGCAGCGCCCTGGGGCAGCTGGGATCCGAAG GCCTGGAGGCAGGTGCCCGCTCCACTACTGCCTAGCTGCGACGCCACAGCAAGAGGAACA GAGATCAGGTCTTATGGCAATGACCCCTGA
Restriction Sites	Please inquire
ACCN	NM_001177466
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001177466.1, NP_001170937.1
RefSeq Size	4533 bp
RefSeq ORF	2250 bp
Locus ID	10046
UniProt ID	Q13495
Gene Summary	This gene encodes a mastermind-like domain containing protein. This protein may function as a transcriptional co-activator. Mutations in this gene are the cause of X-linked hypospadias type 2. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010] Transcript Variant: This variant (3) has a shorter 5' UTR and multiple differences in the coding region, compared to variant 1, one of which results in a translational frameshift. The resulting protein (isoform 3) has a distinct C-terminus and is shorter than isoform 1.

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