RAP80 (UIMC1) (NM_001199298) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	RAP80; X2HRIP110
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC329508 representing NM_001199298. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCACGGAGAAAGAAAAAAGTTAAAGAAGTCTCCGAATCTCGGAACCTGGAGAAGAAGGATGTGGAA ACTACCAGTTCTGTCAGTGTGAAGAGGAAGCGTAGACTTGAGGATGCATTCATTGTGATATCCGATAGT GATGGAGAGGAACCAAAGGAGGAAAATGGGTTGCAGAAAACGAAGACAAAACAGTCGAATAGAGCAAAG TGTTTGGCCAAAAGAAAAATCGCACAGATGACAGAAGAAGAACAGTTTGCTCTGGCTCTCAAAATGAGT GAGCAGGAAGCTAGGGAGGTGAACAGCCAGGAGGAGGAAGAAGAGGAGCTCTTGAGGAAAGCCATTGCT GAAAGCCTGAATAGTTGCCGGCCTTCTGATGCTTCCGCTACCAGATCTCGACCTCTGGCCACTGGACCG TCTTCCCAGTCCCATCAAGAGAAAACCACAGACTCTGGGCTCACTGAAGGCATATGGCAGCTGGTACCT CCATCACTGTTTAAAGGCTCACATATCAGTCAGGGAAACGAGGCTGAGGAAAGAGAGGAGCCTTGGGAC CACACTGAAAAAACTGAAGAGGAGCCGGTCTCTGGCAGCTCAGGAAGCTGGGACCAGTCAAGCCAGCCA GTGTTTGAGAATGTGAACGTTAAATCTTTTGACAGATGTACTGGCCACTCGGCTGAGCACACACAGTGT GGGAAGCCACAGGAAAGTACTGGGAGGGGTTCTGCTTTTCTCAAAGCTGTCCAGGGTAGCGGGGACACA TCTAGGCACTGTCTACCTACCCTAGCAGATGCCAAAGGTCTCCAGGACACTGGGGGCACTGTGAACTAT TTCTGGGGTATTCCATTCTGCCCTGATGGAGTAGACCCTAACCAGTATACCAAGGTCATTCTCTGCCAG TTGGAGGTTTATCAAAAGAGCCTGAAAATGGCTCAGAGGCAGCTCCTTAATAAAAAAGGTTTTGGGGAA CCAGTGTTACCTAGACCTCCTTCTCTGATCCAGAATGAATGTGGCCAAGGAGAGCAGGCTAGTGAGAAA AATGAATGCATCTCAGAAGATATGGGAGATGAAGACAAAGAGGAGAGGCAGGAGTCTAGGGCATCTGAC TGGCACTCAAAAACCAAGGATTTCCAGGAAAGCTCAATTAAAAGCTTGAAAGAGAAACTTTTGTTGGAG GAAGAACCAACAACCAGTCATGGTCAGTCTTCCCAAGGGATTGTTGAAGAAACTTCTGAAGAGGGAAAC TCTGTACCTGCTTCACAAAGTGTTGCTGCTTTGACCAGTAAGAGAAGCTTAGTCCTTATGCCAGAGAGT TCTGCAGAAGAAATCACTGTTTGTCCTGAGACCCAGCTAAGTTCCTCTGAAACTTTTGACCTTGAAAGA GAAGTCTCTCCAGGTAGCAGAGATATCTTGGATGGAGTCAGAATAATAATGGCAGATAAGGAGGTTGGT AACAAGGAAGATGCTGAGAAGGAAGTAGCTATTTCTACCTTCTCATCCAGTAACCAGGTATCCTGCCCG CTATGTGACCAATGCTTTCCACCCACAAAGATTGAACGACATGCCATGTACTGCAATGGTCTGATGGAG GAAGATACAGTATTGACTCGGAGACAAAAAGAGGCCAAGACCAAGAGTGACAGTGGGACAGCTGCCCAG ACTTCTCTAGACATTGACAAGAATGAGAAGTGTTACCTCTGTAAATCCCTGGTCCCATTTAGAGAGTAT CAGTGTCATGTGGACTCCTGTCTCCAGCTTGCAAAGGCTGACCAAGGAGATGGACCTGAAGGGAGTGGA AGAGCATGTTCAACTGTGGAGGGGAAGTGGCAGCAGAGGCTGAAGAACCCAAAGGAAAAAGGCCACAGT GAAGGCCGACTCCTTAGTTTCTTGGAACAGTCTGAGCACAAGACTTCAGATGCAGACATCAAGTCTTCA GAAACAGGAGCCTTCAGGGTGCCTTCACCAGGGATGGAAGAGGCAGGCTGCAGCAGAGAGATGCAGAGT TCTTTCACACGTCGTGACTTAAATGAATCTCCCGTCAAGTCTTTTGTTTCCATTTCAGAAGCCACAGAT TGCTTAGTGGACTTTAAAAAGCAAGTTACTGTCCAGCCAGGTAGTCGGACACGGACCAAAGCTGGCAGA GGAAGAAGGAGAAAATTCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001199298
Insert Size	2160 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001199298.1
RefSeq Size	2615 bp
RefSeq ORF	2160 bp
Locus ID	51720
UniProt ID	Q96RL1
Protein Families	Druggable Genome, Transcription Factors
MW	79.7 kDa
Gene Summary	This gene encodes a nuclear protein that interacts with Brca1 (breast cancer 1) in a complex to recognize and repair DNA lesions. This protein binds ubiquitinated lysine 63 of histone H2A and H2AX. This protein may also function as a repressor of transcription. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015] Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 3 encode the same protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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