Neurobeachin (NBEA) (NM_001204197) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BCL8B; LYST2; NEDEGE
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC329725 representing NM_001204197. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTTCAGCGAGATACGAGCTGTATTTTCAAGACGTTACCTTCTACAAAACACTGCTTTGGAAGTATTT ATGGCAAACCGAACCTCAGTTATGTTTAATTTCCCTGATCAAGCAACAGTAAAAAAAGTTGTCTATAGC TTGCCTCGGGTTGGAGTAGGGACCAGCTATGGTCTGCCACAAGCCAGGAGGATATCATTGGCCACTCCT CGACAGCTTTATAAATCTTCCAATATGACTCAGCGCTGGCAAAGAAGGGAAATTTCAAACTTCGAATAT TTGATGTTCCTTAATACTATTGCAGGACGGACATATAATGATCTGAACCAATATCCAGTGTTTCCGTGG GTGTTAACCAACTATGAATCAGAAGAGTTGGACCTGACTCTTCCAGGAAACTTCAGGGATCTATCAAAG CCAATTGGTGCTTTGAACCCCAAGAGAGCTGTGTTTTATGCAGAGCGTTATGAGACATGGGAAGATGAT CAAAGCCCACCCTACCATTATAATACCCATTATTCAACAGCAACATCTACTTTATCCTGGCTTGTTCGA ATTGAACCTTTCACAACCTTCTTCCTCAATGCAAATGATGGAAAATTTGATCATCCAGATCGAACCTTC TCATCCGTTGCAAGGTCTTGGAGAACTAGTCAGAGAGATACTTCTGATGTAAAGGAACTAATTCCAGAG TTCTACTACCTACCAGAGATGTTTGTCAACAGTAATGGATATAATCTTGGAGTCAGAGAAGATGAAGTA GTGGTAAATGATGTTGATCTTCCCCCTTGGGCAAAAAAACCTGAAGACTTTGTGCGGATCAACAGGATG GCCCTAGAAAGTGAATTTGTTTCTTGCCAACTTCATCAGTGGATCGACCTTATATTTGGCTATAAGCAG CGAGGACCAGAAGCAGTTCGTGCTCTGAATGTTTTTCACTACTTGACTTATGAAGGCTCTGTGAACCTG GATAGTATCACTGATCCTGTGCTCAGGGAGGCCATGGAGGCACAGATACAGAACTTTGGACAGACGCCA TCTCAGTTGCTTATTGAGCCACATCCGCCTCGGAGCTCTGCCATGCACCTGTGTTTCCTTCCACAGAGT CCGCTCATGTTTAAAGATCAGATGCAACAGGATGTGATAATGGTGCTGAAGTTTCCTTCAAATTCTCCA GTAACCCATGTGGCAGCCAACACTCTGCCCCACTTGACCATCCCCGCAGTGGTGACAGTGACTTGCAGC CGACTCTTTGCAGTGAATAGATGGCACAACACAGTAGGCCTCAGAGGAGCTCCAGGATACTCCTTGGAT CAAGCCCACCATCTTCCCATTGAAATGGATCCATTAATAGCCAATAATTCAGGTGTAAACAAACGGCAG ATCACAGACCTCGTTGACCAGAGTATACAAATCAATGCACATTGTTTTGTGGTAACAGCAGATAATCGC TATATTCTTATCTGTGGATTCTGGGATAAGAGCTTCAGAGTTTATTCTACAGAAACAGGGAAATTGACT CAGATTGTATTTGGCCATTGGGATGTGGTCACTTGCTTGGCCAGGTCCGAGTCATACATTGGTGGGGAC TGCTACATCGTGTCCGGATCTCGAGATGCCACCCTGCTGCTCTGGTACTGGAGTGGGCGGCACCATATC ATAGGAGACAACCCTAACAGCAGTGACTATCCGGCACCAAGAGCCGTCCTCACAGGCCATGACCATGAA GTTGTCTGTGTTTCTGTCTGTGCAGAACTTGGGCTTGTTATCAGTGGTGCTAAAGAGGGCCCTTGCCTT GTCCACACCATCACTGGAGATTTGCTGAGAGCCCTTGAAGGACCAGAAAACTGCTTATTCCCACGCTTG ATATCTGTCTCCAGCGAAGGCCACTGTATCATATACTATGAACGAGGGCGATTCAGTAATTTCAGCATT AATGGGAAACTTTTGGCTCAAATGGAGATCAATGATTCAACACGGGCCATTCTCCTGAGCAGTGACGGC CAGAACCTGGTCACCGGAGGGGACAATGGGGTAGTAGAGGTCTGGCAGGCCTGTGACTTCAAGCAACTG TACATTTACCCTGGATGTGATGCTGGCATTAGAGCAATGGACTTGTCCCATGACCAGAGGACTCTGATC ACTGGCATGGCTTCTGGTAGCATTGTAGCTTTTAATATAGATTTTAATCGGTGGCATTATGAGCATCAG AACAGATACTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001204197
Insert Size	2220 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001204197.1
RefSeq Size	4107 bp
RefSeq ORF	2220 bp
Locus ID	26960
UniProt ID	Q8NFP9
MW	84 kDa
Gene Summary	This gene encodes a member of a large, diverse group of A-kinase anchor proteins that target the activity of protein kinase A to specific subcellular sites by binding to its type II regulatory subunits. Brain-specific expression and coat protein-like membrane recruitment of a highly similar protein in mouse suggest an involvement in neuronal post-Golgi membrane traffic. Mutations in this gene may be associated with a form of autism. This gene and its expression are frequently disrupted in patients with multiple myeloma. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants may exist, but their full-length nature has not been determined.[provided by RefSeq, Feb 2011] Transcript Variant: This variant (2) has a novel 5' UTR, lacks multiple 5' coding exons, and uses a downstream in-frame start codon, compared to variant 1. Variant 2 encodes a shorter protein (isoform 2) that lacks a neurobeachin pleckstrin homology-like domain and DUF1088 domain, compared to isoform 1.

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