Transcription termination factor 1 (TTF1) (NM_001205296) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	TTF-1; TTF-I
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC329802 representing NM_001205296. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTACCGGGACGACTTGGAACGGTTTAAGGAATTTAAAGCACAAGGTGTCGCTATTAAATTTGGCAAG TTTTCTGTAAAGGAAAATAAGCAGTTAGAGAAAAATGTGGAAGACTTTCTAGCCCTGACAGGCATTGAG AGTGCAGACAAGCTGCTGTACACGGACAGATATCCTGAGGAAAAATCTGTGATCACCAACTTAAAAAGG AGATACTCGTTTAGATTACACATTGGTAGGAACATTGCCCGGCCCTGGAAACTTATATACTATCGAGCA AAGAAGATGTTCGATGTCAACAATTACAAAGGCAGGTATAGCGAAGGAGATACTGAGAAGTTAAAGATG TACCATTCTCTCCTTGGGAATGACTGGAAGACGATTGGTGAGATGGTGGCCCGAAGTAGCCTCTCCGTG GCCCTCAAGTTCTCACAGATCAGCAGTCAAAGAAATCGTGGTGCTTGGAGTAAGTCTGAAACCCGGAAA CTAATCAAGGCTGTCGAAGAAGTGATTCTGAAGAAGATGTCTCCCCAGGAGTTAAAAGAGGTGGATTCC AAACTCCAAGAAAATCCTGAAAGTTGCCTATCAATTGTTCGGGAAAAACTCTACAAGGGCATATCTTGG GTAGAAGTAGAAGCTAAAGTGCAAACCAGAAATTGGATGCAGTGTAAAAGTAAGTGGACAGAAATTCTA ACCAAGAGGATGACTAATGGTCGGCGTATCTACTATGGCATGAATGCCCTGCGGGCCAAGGTCAGCCTT ATTGAAAGGTTGTATGAAATAAATGTGGAAGATACTAATGAAATAGACTGGGAAGATCTTGCTAGTGCC ATAGGTGATGTTCCTCCATCTTACGTTCAAACTAAATTTTCTAGGCTGAAAGCTGTCTATGTTCCATTT TGGCAGAAAAAGACTTTTCCAGAGATCATCGACTACCTTTATGAGACGACTCTACCTTTGCTGAAGGAA AAGTTAGAAAAAATGATGGAGAAAAAAGGCACTAAAATCCAGACTCCTGCAGCACCCAAGCAAGTTTTC CCATTTCGAGACATCTTTTATTATGAAGACGATAGTGAAGGAGAGGACATAGAAAAAGAAAGCGAAGGC CAGGCGCCATGCATGGCTCACGCCTGTAATTCCAGTACTTTGGGAGGCCAAGGCCGGTGGATCATCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001205296
Insert Size	1173 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001205296.1
RefSeq Size	1780 bp
RefSeq ORF	1173 bp
Locus ID	7270
UniProt ID	Q15361
MW	45.6 kDa
Gene Summary	This gene encodes a transcription termination factor that is localized to the nucleolus and plays a critical role in ribosomal gene transcription. The encoded protein mediates the termination of RNA polymerase I transcription by binding to Sal box terminator elements downstream of pre-rRNA coding regions. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. This gene shares the symbol/alias 'TFF1' with another gene, NK2 homeobox 1, also known as thyroid transcription factor 1, which plays a role in the regulation of thyroid-specific gene expression. [provided by RefSeq, Apr 2011] Transcript Variant: This variant (2) lacks an exon in the 5' coding region and initiates translation at a downstream, in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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