Transcription termination factor 1 (TTF1) (NM_001205296) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	TTF-1; TTF-I
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC232638 representing NM_001205296 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACCGGGACGACTTGGAACGGTTTAAGGAATTTAAAGCACAAGGTGTCGCTATTAAATTTGGCAAGT TTTCTGTAAAGGAAAATAAGCAGTTAGAGAAAAATGTGGAAGACTTTCTAGCCCTGACAGGCATTGAGAG TGCAGACAAGCTGCTGTACACGGACAGATATCCTGAGGAAAAATCTGTGATCACCAACTTAAAAAGGAGA TACTCGTTTAGATTACACATTGGTAGGAACATTGCCCGGCCCTGGAAACTTATATACTATCGAGCAAAGA AGATGTTCGATGTCAACAATTACAAAGGCAGGTATAGCGAAGGAGATACTGAGAAGTTAAAGATGTACCA TTCTCTCCTTGGGAATGACTGGAAGACGATTGGTGAGATGGTGGCCCGAAGTAGCCTCTCCGTGGCCCTC AAGTTCTCACAGATCAGCAGTCAAAGAAATCGTGGTGCTTGGAGTAAGTCTGAAACCCGGAAACTAATCA AGGCTGTCGAAGAAGTGATTCTGAAGAAGATGTCTCCCCAGGAGTTAAAAGAGGTGGATTCCAAACTCCA AGAAAATCCTGAAAGTTGCCTATCAATTGTTCGGGAAAAACTCTACAAGGGCATATCTTGGGTAGAAGTA GAAGCTAAAGTGCAAACCAGAAATTGGATGCAGTGTAAAAGTAAGTGGACAGAAATTCTAACCAAGAGGA TGACTAATGGTCGGCGTATCTACTATGGCATGAATGCCCTGCGGGCCAAGGTCAGCCTTATTGAAAGGTT GTATGAAATAAATGTGGAAGATACTAATGAAATAGACTGGGAAGATCTTGCTAGTGCCATAGGTGATGTT CCTCCATCTTACGTTCAAACTAAATTTTCTAGGCTGAAAGCTGTCTATGTTCCATTTTGGCAGAAAAAGA CTTTTCCAGAGATCATCGACTACCTTTATGAGACGACTCTACCTTTGCTGAAGGAAAAGTTAGAAAAAAT GATGGAGAAAAAAGGCACTAAAATCCAGACTCCTGCAGCACCCAAGCAAGTTTTCCCATTTCGAGACATC TTTTATTATGAAGACGATAGTGAAGGAGAGGACATAGAAAAAGAAAGCGAAGGCCAGGCGCCATGCATGG CTCACGCCTGTAATTCCAGTACTTTGGGAGGCCAAGGCCGGTGGATCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC232638 representing NM_001205296 Red=Cloning site Green=Tags(s) MYRDDLERFKEFKAQGVAIKFGKFSVKENKQLEKNVEDFLALTGIESADKLLYTDRYPEEKSVITNLKRR YSFRLHIGRNIARPWKLIYYRAKKMFDVNNYKGRYSEGDTEKLKMYHSLLGNDWKTIGEMVARSSLSVAL KFSQISSQRNRGAWSKSETRKLIKAVEEVILKKMSPQELKEVDSKLQENPESCLSIVREKLYKGISWVEV EAKVQTRNWMQCKSKWTEILTKRMTNGRRIYYGMNALRAKVSLIERLYEINVEDTNEIDWEDLASAIGDV PPSYVQTKFSRLKAVYVPFWQKKTFPEIIDYLYETTLPLLKEKLEKMMEKKGTKIQTPAAPKQVFPFRDI FYYEDDSEGEDIEKESEGQAPCMAHACNSSTLGGQGRWII TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001205296
ORF Size	1170 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001205296.1, NP_001192225.1
RefSeq Size	1780 bp
RefSeq ORF	1173 bp
Locus ID	7270
UniProt ID	Q15361
MW	46.1 kDa
Gene Summary	This gene encodes a transcription termination factor that is localized to the nucleolus and plays a critical role in ribosomal gene transcription. The encoded protein mediates the termination of RNA polymerase I transcription by binding to Sal box terminator elements downstream of pre-rRNA coding regions. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. This gene shares the symbol/alias 'TFF1' with another gene, NK2 homeobox 1, also known as thyroid transcription factor 1, which plays a role in the regulation of thyroid-specific gene expression. [provided by RefSeq, Apr 2011]

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