DIS3L2 (NM_001257281) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FAM6A; hDIS3L2; PRLMNS
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330592 representing NM_001257281. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAGCCATCCTGACTACAGAATGAACCTCCGGCCCCTGGGGACCCCCAGAGGTGTGTCTGCTGTGGCT GGTCCACATGACATTGGTGCTTCGCCAGGTGACAAAAAGTCAAAGAACAGGTCCACACGAGGGAAGAAA AAGAGCATATTTGAAACTTACATGTCCAAGGAGGATGTTTCAGAAGGCTTGAAGAGAGGAACACTCATC CAGGGTGTATTGAGAATTAATCCAAAGAAGTTTCATGAAGCCTTCATTCCTTCCCCGGATGGTGATCGA GACATTTTTATTGATGGGGTTGTTGCTCGTAATAGAGCCTTAAATGGGGATCTGGTGGTCGTGAAACTG CTTCCCGAGGAGCATTGGAAGGTAGTTAAACCAGAGAGCAATGACAAAGAAACAGAAGCTGCGTATGAA TCAGATATCCCCGAGGAGCTCTGTGGACACCATCTCCCGCAACAGTCCCTGAAAAGCTATAATGACAGT CCTGATGTCATTGTAGAGGCTCAGTTTGATGGCAGCGACTCAGAAGATGGACATGGCATCACACAAAAT GTGCTGGTTGATGGTGTTAAGAAACTCTCAGTTTGTGTTTCTGAGAAAGGAAGAGAGGATGGTGATGCA CCGGTTACAAAAGATGAGACCACCTGCATTTCACAAGACACAAGAGCTTTATCGGAGAAATCCCTGCAA AGATCAGCAAAGGTGGTTTACATCTTGGAGAAAAAACATTCTCGAGCAGCAACCGGCTTCCTCAAACTC TTGGCTGATAAGAACAGCGAACTGTTTAGGAAATACGCCCTGTTTTCTCCCTCAGACCACCGAGTGCCT AGAATTTATGTGCCTCTCAAGGACTGTCCCCAGGACTTTGTGGCACGGCCTAAAGATTATGCCAACACA CTGTTCATCTGCCGCATTGTGGACTGGAAGGAGGACTGCAATTTTGCCCTGGGGCAGCTGGCTAAGAGT CTTGGGCAGGCTGGTGAAATTGAGCCTGAAACAGAAGGAATACTAACAGAGTATGGCGTGGATTTCTCT GATTTCTCTTCAGAAGTTCTAGAATGTCTTCCTCAAGGCCTGCCATGGACAATTCCACCAGAGGAGTTC AGCAAGAGAAGGGATTTAAGAAAAGACTGTATCTTCACCATTGACCCATCAACCGCCCGAGACCTCGAT GATGCCCTCTCCTGCAAGCCACTCGCTGACGGCAACTTCAAAGTGGGAGTTCACATTGCTGACGTGAGT TACTTTGTTCCGGAGGGATCTGATCTGGATAAAGTGGCTGCCGAGAGGGCTACAAGCGTCTACTTGGTT CAAAAGGTGGTCCCCATGCTTCCCAGGCTGCTGTGTGAGGAGCTGTGCAGCCTCAACCCCATGTCCGAC AAGCTGACCTTCTCTGTGATCTGGACACTGACTCCAGAGGGCAAGATCCTTGATGAATGGTTTGGCCGG ACCATCATCCGCTCCTGCACCAAACTTAGCTACGAGCATGCACAGAGCATGATTGAAAGCCCAACTGAG AAAATCCCTGCGAAAGAGCTGCCCCCCATTTCCCCAGAGCATAGCAGCGAGGAGGTACACCAGCAGAAC GCAGACAAGGATGGGGCTGCCCATCTTCAGGCCTCTCACAGCCCCTCTGCTGAAGATGCAGAAGCACAG CCCTCCACAGAGGAACGCCTGCCTGAGACTCGGGGCATATGTGACAGGGATCCAGACACACGACTGTTT TTCCTTCAGCAACAGAGCCGTGTATTGGAAGCAAAGCCCCAAAACACGATAAGAGTAGAGGAGCAGACA ACCCAGTTGCAGATTTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001257281
Insert Size	1812 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001257281.1
RefSeq Size	2481 bp
RefSeq ORF	1812 bp
Locus ID	129563
UniProt ID	Q8IYB7
MW	67.3 kDa
Gene Summary	The protein encoded by this gene is similar in sequence to 3'/5' exonucleolytic subunits of the RNA exosome. The exosome is a large multimeric ribonucleotide complex responsible for degrading various RNA substrates. Several transcript variants, some protein-coding and some not, have been found for this gene. [provided by RefSeq, Mar 2012] Transcript Variant: This variant (2) lacks several exons and includes an alternate 3' terminal exon compared to variant 1. It encodes isoform 2, which is shorter and has a distinct C-terminus compared to isoform 1.

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