CLPB (NM_001258394) Human Untagged Clone
CAT#: SC330677
CLPB (untagged) - Homo sapiens ClpB caseinolytic peptidase B homolog (E. coli) (CLPB), transcript variant 4
CNY 6,460.00
货期*
4周
规格
Product images
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经常一起买 (4)
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | ANKCLB; HSP78; MEGCANN; MGCA7; SKD3 |
Vector | pCMV6-Entry |
Sequence Data |
>SC330677 representing NM_001258394.
Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCTCGCGGCTGCCACTTGGGGACGCCTTCCTGGTCCCGAAGAAACACTCCCAGGACAGGACAGCTG GAACGGGGTCCCCAGCAGGGCCGGACTGGGCATGTGCGCCCTGGCCGCAGCGCTGGTGGTTCATTGCTA CAGCAAGAGTCCGTCCAACAAGGATGCAGCCCTGTTGGAAGCTGCCCGTGCCAACAATATGCAAGAAGT CAGCAGCCCCAAGAGACTGCAAAGAATGATGCTCAGTCAAGAAGCTGGGCAGGGCTGAATGCTGGAGTC TCACTGAAGAACACCAAGATTTCCTCCTCTGAATGGCCCCTCAGGCTGTTGTCAGAAGGTGCAGATGTC AATGCAAAGCACAGACTTGGCTGGACAGCACTCATGGTGGCAGCCATCAACCGAAACAACAGTGTGGTA CAGGTCCTGCTTGCTGCTGGGGCTGATCCAAACCTTGGAGATGATTTCAGCAGTGTTTACAAGACTGCC AAGGAACAGGGAATCCATTCTTTGGAAGATGGGGGACAGGACGGTGCAAGCCGGCACATCACAAACCAG TGGACAAGTGCCCTGGAGTTCAGGAGATGGCTAGGACTCCCCGCTGGCGTCCTGATCACCCGAGAGGAT GACTTCAACAACAGGCTGAACAACCGCGCCAGTTTCAAGGGCTGCACGGCCTTGCACTATGCTGTTCTT GCTGATGACTACCGCACTGTCAAGGAGCTGCTTGATGGAGGAGCCAACCCCCTGCAGAGGAATGAAATG GGACACACACCCTTGGATTATGCCCGAGAAGGGGAAGTGATGAAGCTTCTGAGGACTTCTGAAGCCAAG TACCAAGAGAAGCAGCGGAAGCGTGAGGCTGAGGAGCGGCGCCGCTTCCCCCTGGAGCAGCGACTAAAG GAGCACATCATTGGCCAGGAGAGCGCCATCGCCACAGTGGGTGCTGCGATCCGGAGGAAGGAGAATGGC TGGTACGATGAAGAACACCCTCTGGTCTTCCTCTTCTTGGGATCATCTGGAATAGGAAAAACAGAGCTG GCCAAGCAGACAGCCAAATATATGCACAAAGATGCTAAAAAGGGCTTCATCAGGCTGGACATGTCCGAG TTCCAGGAGCGACACGAGGTGGCCAAGTTTATTGGGTCTCCACCAGGCTACGTTGGCCATGAGGAGGGT GGCCAGCTGACCAAGAAGTTGAAGCAGTGCCCCAATGCTGTGGTGCTCTTTGATGAAGTAGACAAGGCC CATCCAGATGTGCTCACCATCATGCTGCAGCTGTTTGATGAGGGCCGGCTGACAGATGGAAAAGGGAAG ACCATTGATTGCAAGGACGCCATCTTCATCATGACCTCCAATGTGGCCAGCGACGAGATCGCACAGCAC GCGCTGCAGCTGAGGCAGGAAGCTTTGGAGATGAGCCGTAACCGTATTGCCGAAAACCTGGGGGATGTC CAGATAAGTGACAAGATCACCATCTCAAAGAACTTCAAGGAGAATGTGATTCGCCCTATCCTGAAAGCT CACTTCCGGAGGGATGAGTTTCTGGGACGGATCAATGAGATCGTCTACTTCCTCCCCTTCTGCCACTCG GAGCTCATCCAACTCGTCAACAAGGAACTAAACTTCTGGGCCAAGAGAGCCAAGCAAAGGCACAACATC ACGCTGCTCTGGGACCGCGAGGTGGCAGATGTGCTGGTCGACGGCTACAATGTGCACTATGGCGCCCGC TCCATCAAACATGAGGTAGAACGCCGTGTGGTGAACCAGCTGGCAGCAGCCTATGAGCAGGACCTGCTG CCAGGGGGCTGTACTTTGCGCATCACGGTGGAGGACTCAGACAAGCAGCTACTCAAAAGCCCAGAACTG CCCTCACCCCAGGCTGAGAAGCGCCTCCCCAAGCTGCGTCTGGAGATCATCGACAAGGACAGCAAGACT CGCAGACTGGACATCCGGGCACCACTGCACCCTGAGAAGGTGTGCAACACCATCTAG |
Restriction Sites | SgfI-MluI |
ACCN | NM_001258394 |
Insert Size | 1989 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001258394.1 |
RefSeq Size | 3379 bp |
RefSeq ORF | 1989 bp |
Locus ID | 81570 |
UniProt ID | Q9H078 |
MW | 74.5 kDa |
Gene Summary | This gene belongs to the ATP-ases associated with diverse cellular activities (AAA+) superfamily. Members of this superfamily form ring-shaped homo-hexamers and have highly conserved ATPase domains that are involved in various processes including DNA replication, protein degradation and reactivation of misfolded proteins. All members of this family hydrolyze ATP through their AAA+ domains and use the energy generated through ATP hydrolysis to exert mechanical force on their substrates. In addition to an AAA+ domain, the protein encoded by this gene contains a C-terminal D2 domain, which is characteristic of the AAA+ subfamily of Caseinolytic peptidases to which this protein belongs. It cooperates with Hsp70 in the disaggregation of protein aggregates. Allelic variants of this gene are associated with 3-methylglutaconic aciduria, which causes cataracts and neutropenia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015] Transcript Variant: This variant (4) differs in the 5' UTR and contains an alternate exon in the 5' coding region that results in initiation of translation at an alternate start codon and a novel 5' coding region compared to variant 1. The encoded isoform (4) is shorter than and has a distinct N-terminus compared to isoform 1. |
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