CRLR (CALCRL) (NM_001271751) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CGRPR; CRLR; LMPHM8
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330964 representing NM_001271751. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAGAAAAAGTGTACCCTGTATTTTCTGGTTCTCTTGCCTTTTTTTATGATTCTTGTTACAGCAGAA TTAGAAGAGAGTCCTGAGGACTCAATTCAGTTGGGAGTTACTAGAAATAAAATCATGACAGCTCAATAT GAATGTTACCAAAAGATTATGCAAGACCCCATTCAACAAGCAGAAGGCGTTTACTGCAACAGAACCTGG GATGGATGGCTCTGCTGGAACGATGTTGCAGCAGGAACTGAATCAATGCAGCTCTGCCCTGATTACTTT CAGGACTTTGATCCATCAGAAAAAGTTACAAAGATCTGTGACCAAGATGGAAACTGGTTTAGACATCCA GCAAGCAACAGAACATGGACAAATTATACCCAGTGTAATGTTAACACCCACGAGAAAGTGAAGACTGCA CTAAATTTGTTTTACCTGACCATAATTGGACACGGATTGTCTATTGCATCACTGCTTATCTCGCTTGGC ATATTCTTTTATTTCAAGAGCCTAAGTTGCCAAAGGATTACCTTACACAAAAATCTGTTCTTCTCATTT GTTTGTAACTCTGTTGTAACAATCATTCACCTCACTGCAGTGGCCAACAACCAGGCCTTAGTAGCCACA AATCCTGTTAGTTGCAAAGTGTCCCAGTTCATTCATCTTTACCTGATGGGCTGTAATTACTTTTGGATG CTCTGTGAAGGCATTTACCTACACACACTCATTGTGGTGGCCGTGTTTGCAGAGAAGCAACATTTAATG TGGTATTATTTTCTTGGCTGGGGATTTCCACTGATTCCTGCTTGTATACATGCCATTGCTAGAAGCTTA TATTACAATGACAATTGCTGGATCAGTTCTGATACCCATCTCCTCTACATTATCCATGGCCCAATTTGT GCTGCTTTACTGGTGAATCTTTTTTTCTTGTTAAATATTGTACGCGTTCTCATCACCAAGTTAAAAGTT ACACACCAAGCGGAATCCAATCTGTACATGAAAGCTGTGAGAGCTACTCTTATCTTGGTGCCATTGCTT GGCATTGAATTTGTGCTGATTCCATGGCGACCTGAAGGAAAGATTGCAGAGGAGGTATATGACTACATC ATGCACATCCTTATGCACTTCCAGGGTCTTTTGGTCTCTACCATTTTCTGCTTCTTTAATGGAGAGGTT CAAGCAATTCTGAGAAGAAACTGGAATCAATACAAAATCCAATTTGGAAACAGCTTTTCCAACTCAGAA GCTCTTCGTAGTGCGTCTTACACAGTGTCAACAATCAGTGATGGTCCAGGTTATAGTCATGACTGTCCT AGTGAACACTTAAATGGAAAAAGCATCCATGATATTGAAAATGTTCTCTTAAAACCAGAAAATTTATAT AATTGA
Restriction Sites	SgfI-RsrII
ACCN	NM_001271751
Insert Size	1386 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271751.1
RefSeq Size	5990 bp
RefSeq ORF	1386 bp
Locus ID	10203
UniProt ID	Q16602
Protein Families	Druggable Genome, GPCR, Transmembrane
Protein Pathways	Neuroactive ligand-receptor interaction, Vascular smooth muscle contraction
MW	53 kDa
Gene Summary	Receptor for calcitonin-gene-related peptide (CGRP) together with RAMP1 and receptor for adrenomedullin together with RAMP3 (By similarity). Receptor for adrenomedullin together with RAMP2. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an exon in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. An in-frame AUG is located 11 codons upstream of the annotated translation start site but is not being annotated as a start site since it is not conserved and is in a weak Kozak sequence context.

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