MBD1 (NM_001204140) Human Untagged Clone

CAT#: SC331499

MBD1 (untagged) - Homo sapiens methyl-CpG binding domain protein 1 (MBD1), transcript variant 9



  "NM_001204140" in other vectors (2)

CNY 5,610.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
MBD1 mouse monoclonal antibody,clone OTI2H5
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms CXXC3; PCM1; RFT
Vector pCMV6-Entry
Sequence Data
>SC331499 representing NM_001204140.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

ATGGCTGAGGACTGGCTGGACTGCCCGGCCCTGGGCCCTGGCTGGAAGCGCCGCGAAGTCTTTCGCAAG
TCAGGGGCCACCTGTGGACGCTCAGACACCTATTACCAGAGCCCCACAGGAGACAGGATCCGAAGCAAA
GTTGAGCTGACTCGATACCTGGGCCCTGCGTGTGATCTCACCCTCTTCGACTTCAAACAAGGCATCTTG
TGCTATCCAGCCCCCAAGGCCCATCCCGTGGCGGTTGCCAGCAAGAAGCGAAAGAAGCCTTCAAGGCCA
GCCAAGACTCGGAAACGTCAGGTTGGACCCCAGAGTGGTGAGGTCAGGAAGGAGGCCCCGAGGGATGAG
ACCAAGGCTGACACTGACACAGCCCCAGCTTCATTCCCTGCTCCTGGGTGCTGTGAGAACTGTGGAATC
AGCTTCTCAGGGGATGGCACCCAAAGGCAGCGGCTCAAAACGTTGTGCAAAGACTGTCGAGCACAGAGA
ATTGCCTTCAACCGGGAACAGAGAATGTTTAAGCGTGTGGGCTGTGGGGAGTGTGCAGCCTGCCAGGTA
ACAGAAGACTGTGGGGCCTGCTCCACCTGCCTCCTGCAGCTGCCCCATGATGTGGCATCGGGGCTGTTC
TGCAAGTGTGAACGGAGACGCTGCCTCCGGATTGTGGAAAGGAGCCGAGGGTGTGGAGTATGCCGGGGC
TGTCAGACCCAAGAGGATTGTGGCCATTGCCCCATCTGCCTTCGCCCTCCCCGCCCTGGTCTCAGGCGC
CAGTGGAAATGTGTCCAGCGACGTTGCCTACGGCACCTTGCTCACCGCCTGCGTCGCCGTCATCAGAGA
TGTCAGCGACGCACTCCCCTGGCTGTGGCTCCCCCAACTGGTAAACATGCCCGCCGCAAGGGAGGCTGT
GACTCCAAGATGGCTGCCAGGCGGCGCCCCGGAGCCCAGCCACTGCCTCCACCACCCCCATCACAGTCC
CCAGAGCCCACAGAGCCGCACCCCAGAGCCCTGGCCCCCTCGCCACCTGCCGAGTTCATCTATTACTGT
GTAGACGAGGACGAGCTAAAGCGGCTGCTGCCCAGTGTCTGGTCAGAGTCTGAGGATGGGGCAGGATCG
CCCCCACCTTACCGTCGTCGAAAGAGGCCCAGCTCTGCCCGACGGCACCATCTTGGCCCTACCTTGAAG
CCCACCTTGGCTACACGCACAGCCCAACCAGACCATACCCAGGCTCCAACGAAGCAGGAAGCAGGTGGT
GGCTTTGTGCTGCCCCCGCCTGGCACTGACCTTGTGTTTTTACGGGAAGGCGCAAGCAGTCCTGTGCAG
GTGCCGGGCCCTGTTGCAGCTTCCACAGAAGCCCTGTTGCAGGAGGCCCAGTGCTCTGGCCTGAGTTGG
GTTGTGGCCTTACCCCAGGTGAAGCAAGAGAAGGCGGATACCCAGGACGAGTGGACACCAGGCACAGCT
GTCCTGACTTCTCCCGTATTGGTGCCTGGCTGCCCTAGCAAGGCAGTAGACCCAGGCCTGCCTTCTGTG
AAGCAAGAGCCACCTGACCCAGAGGAGGACAAGGAGGAGAACAAGGATGATTCTGCCTCCAAATTGGCC
CCAGAGGAAGAGGCAGGAGGGGCTGGCACACCCGTGATCACGGAGATTTTCAGCCTGGGTGGAACCCGC
TTCCGAGATACAGCAGTCTGGTTGCCAAGGTCCAAAGACCTTAAAAAACCTGGAGCTAGAAAGCAGTAG

Restriction Sites SgfI-MluI     
ACCN NM_001204140
Insert Size 1725 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001204140.1
RefSeq Size 2628 bp
RefSeq ORF 1725 bp
Locus ID 4152
UniProt ID Q9UIS9
Protein Families Druggable Genome, Transcription Factors
MW 63 kDa
Gene Summary The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains multiple domains: MBD at the N-terminus that functions both in binding to methylated DNA and in protein interactions; several CXXC-type zinc finger domains that mediate binding to non-methylated CpG dinucleotides; transcriptional repression domain (TRD) at the C-terminus that is involved in transcription repression and in protein interactions. Numerous alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Feb 2011]
Transcript Variant: This variant (9) is the same as variant 7, except lacks an in-frame coding exon, resulting in an isoform (9, also known as RFT-B) that is missing an internal protein segment compared to isoform 7. Unlike isoform 7, this isoform fails to bind FGF-2 promoter and repress FGF-2 gene expression (PMID:10187827).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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