MBD1 (NM_001204140) Human Untagged Clone
CAT#: SC331499
MBD1 (untagged) - Homo sapiens methyl-CpG binding domain protein 1 (MBD1), transcript variant 9
CNY 5,610.00
货期*
5周
规格
Product images
经常一起买 (4)
MBD1 mouse monoclonal antibody,clone OTI2H5
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | CXXC3; PCM1; RFT |
Vector | pCMV6-Entry |
Sequence Data |
>SC331499 representing NM_001204140.
Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCTGAGGACTGGCTGGACTGCCCGGCCCTGGGCCCTGGCTGGAAGCGCCGCGAAGTCTTTCGCAAG TCAGGGGCCACCTGTGGACGCTCAGACACCTATTACCAGAGCCCCACAGGAGACAGGATCCGAAGCAAA GTTGAGCTGACTCGATACCTGGGCCCTGCGTGTGATCTCACCCTCTTCGACTTCAAACAAGGCATCTTG TGCTATCCAGCCCCCAAGGCCCATCCCGTGGCGGTTGCCAGCAAGAAGCGAAAGAAGCCTTCAAGGCCA GCCAAGACTCGGAAACGTCAGGTTGGACCCCAGAGTGGTGAGGTCAGGAAGGAGGCCCCGAGGGATGAG ACCAAGGCTGACACTGACACAGCCCCAGCTTCATTCCCTGCTCCTGGGTGCTGTGAGAACTGTGGAATC AGCTTCTCAGGGGATGGCACCCAAAGGCAGCGGCTCAAAACGTTGTGCAAAGACTGTCGAGCACAGAGA ATTGCCTTCAACCGGGAACAGAGAATGTTTAAGCGTGTGGGCTGTGGGGAGTGTGCAGCCTGCCAGGTA ACAGAAGACTGTGGGGCCTGCTCCACCTGCCTCCTGCAGCTGCCCCATGATGTGGCATCGGGGCTGTTC TGCAAGTGTGAACGGAGACGCTGCCTCCGGATTGTGGAAAGGAGCCGAGGGTGTGGAGTATGCCGGGGC TGTCAGACCCAAGAGGATTGTGGCCATTGCCCCATCTGCCTTCGCCCTCCCCGCCCTGGTCTCAGGCGC CAGTGGAAATGTGTCCAGCGACGTTGCCTACGGCACCTTGCTCACCGCCTGCGTCGCCGTCATCAGAGA TGTCAGCGACGCACTCCCCTGGCTGTGGCTCCCCCAACTGGTAAACATGCCCGCCGCAAGGGAGGCTGT GACTCCAAGATGGCTGCCAGGCGGCGCCCCGGAGCCCAGCCACTGCCTCCACCACCCCCATCACAGTCC CCAGAGCCCACAGAGCCGCACCCCAGAGCCCTGGCCCCCTCGCCACCTGCCGAGTTCATCTATTACTGT GTAGACGAGGACGAGCTAAAGCGGCTGCTGCCCAGTGTCTGGTCAGAGTCTGAGGATGGGGCAGGATCG CCCCCACCTTACCGTCGTCGAAAGAGGCCCAGCTCTGCCCGACGGCACCATCTTGGCCCTACCTTGAAG CCCACCTTGGCTACACGCACAGCCCAACCAGACCATACCCAGGCTCCAACGAAGCAGGAAGCAGGTGGT GGCTTTGTGCTGCCCCCGCCTGGCACTGACCTTGTGTTTTTACGGGAAGGCGCAAGCAGTCCTGTGCAG GTGCCGGGCCCTGTTGCAGCTTCCACAGAAGCCCTGTTGCAGGAGGCCCAGTGCTCTGGCCTGAGTTGG GTTGTGGCCTTACCCCAGGTGAAGCAAGAGAAGGCGGATACCCAGGACGAGTGGACACCAGGCACAGCT GTCCTGACTTCTCCCGTATTGGTGCCTGGCTGCCCTAGCAAGGCAGTAGACCCAGGCCTGCCTTCTGTG AAGCAAGAGCCACCTGACCCAGAGGAGGACAAGGAGGAGAACAAGGATGATTCTGCCTCCAAATTGGCC CCAGAGGAAGAGGCAGGAGGGGCTGGCACACCCGTGATCACGGAGATTTTCAGCCTGGGTGGAACCCGC TTCCGAGATACAGCAGTCTGGTTGCCAAGGTCCAAAGACCTTAAAAAACCTGGAGCTAGAAAGCAGTAG |
Restriction Sites | SgfI-MluI |
ACCN | NM_001204140 |
Insert Size | 1725 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001204140.1 |
RefSeq Size | 2628 bp |
RefSeq ORF | 1725 bp |
Locus ID | 4152 |
UniProt ID | Q9UIS9 |
Protein Families | Druggable Genome, Transcription Factors |
MW | 63 kDa |
Gene Summary | The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains multiple domains: MBD at the N-terminus that functions both in binding to methylated DNA and in protein interactions; several CXXC-type zinc finger domains that mediate binding to non-methylated CpG dinucleotides; transcriptional repression domain (TRD) at the C-terminus that is involved in transcription repression and in protein interactions. Numerous alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Feb 2011] Transcript Variant: This variant (9) is the same as variant 7, except lacks an in-frame coding exon, resulting in an isoform (9, also known as RFT-B) that is missing an internal protein segment compared to isoform 7. Unlike isoform 7, this isoform fails to bind FGF-2 promoter and repress FGF-2 gene expression (PMID:10187827). |
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